Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 26, 2024
pH
and
peroxynitrite
(ONOO–)
are
two
critical
biomarkers
to
unveil
the
corresponding
status
of
endoplasmic
reticulum
(ER)
stress
mitochondrial
dysfunction,
which
closely
related
Alzheimer's
disease
(AD).
Simultaneously
monitoring
ONOO–
fluctuations
in
ER
mitochondria
during
AD
progression
is
pivotal
for
clarifying
interplay
between
disorders
organelles
revealing
pathogenesis.
Herein,
we
designed
synthesized
a
dual-channel
fluorescent
probe
(DCFP)
visualize
mitochondria.
DCFP
possessed
excellent
sensitivity
selectivity
without
spectral
crosstalk
was
utilized
analytes
within
model
cells
larval
zebrafish.
Importantly,
could
preferentially
target
normal
be
enriched
after
depolarization.
With
aid
DCFP,
slower
acidification
rate
than
that
induced
by
Aβ
oligomers
(AβOs)
first
identified,
ascribed
relief
AβOs-triggered
through
Ca2+
migration
from
Moreover,
continuous
exposure
AβOs
led
overload,
accelerating
overproduction
As
result,
intracellular
oxidative
levels
were
elevated,
further
exacerbating
aggravating
turn.
The
advanced
understanding
potential
this
work
may
offer
new
insights
methodologies
studying
developed
also
employed
study
other
diseases
dysfunction.
Journal of Materials Chemistry B,
Journal Year:
2024,
Volume and Issue:
12(39), P. 10004 - 10011
Published: Jan. 1, 2024
Current
clinical
indicators
for
assessing
liver/kidney
injury
are
functional
rather
than
indicators,
which
may
cause
some
delays
in
the
diagnosis
of
drug-induced
liver
(DILI)
and
kidney
(DIKI).
ACS Sensors,
Journal Year:
2024,
Volume and Issue:
9(12), P. 6750 - 6758
Published: Nov. 14, 2024
Tendinopathy
is
one
of
the
most
prevalent
musculoskeletal
disorders,
significantly
affecting
quality
life
patients.
Treatment
outcomes
can
be
improved
with
an
early
diagnosis
and
timely
targeted
interventions.
Increasing
evidence
indicates
that
ROS
endoplasmic
reticulum
(ER)
stress
play
key
roles
in
modulating
differentiation
processes
tendon-derived
stem
cells
(TDSCs),
thereby
contributing
to
initiation
progression
tendinopathy.
However,
relationship
between
ONOO-
process,
as
well
various
stages
tendinopathy,
remains
unexplored.
Herein,
we
developed
two
highly
specific
sensitive
fluorescent
probes
(Rod-Cl
Rod-Br)
for
detecting
ER.
Rod-Br
detect
basal
levels
ER
TDSCs
measure
primary
stimulated
by
interleukin-1β
over
durations,
allowing
comparisons
chondrogenic
osteogenic
levels.
Additionally,
examined
variations
different
tendinopathy
treatment
rat
models
vivo
discussed
potential
mechanisms.
This
research
provides
a
robust
tool
analyzing
dynamics
tenogenic
TDSCs,
offering
new
insights
into
pathophysiology
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 26, 2024
pH
and
peroxynitrite
(ONOO–)
are
two
critical
biomarkers
to
unveil
the
corresponding
status
of
endoplasmic
reticulum
(ER)
stress
mitochondrial
dysfunction,
which
closely
related
Alzheimer's
disease
(AD).
Simultaneously
monitoring
ONOO–
fluctuations
in
ER
mitochondria
during
AD
progression
is
pivotal
for
clarifying
interplay
between
disorders
organelles
revealing
pathogenesis.
Herein,
we
designed
synthesized
a
dual-channel
fluorescent
probe
(DCFP)
visualize
mitochondria.
DCFP
possessed
excellent
sensitivity
selectivity
without
spectral
crosstalk
was
utilized
analytes
within
model
cells
larval
zebrafish.
Importantly,
could
preferentially
target
normal
be
enriched
after
depolarization.
With
aid
DCFP,
slower
acidification
rate
than
that
induced
by
Aβ
oligomers
(AβOs)
first
identified,
ascribed
relief
AβOs-triggered
through
Ca2+
migration
from
Moreover,
continuous
exposure
AβOs
led
overload,
accelerating
overproduction
As
result,
intracellular
oxidative
levels
were
elevated,
further
exacerbating
aggravating
turn.
The
advanced
understanding
potential
this
work
may
offer
new
insights
methodologies
studying
developed
also
employed
study
other
diseases
dysfunction.
