Journal of Analytical Chemistry, Journal Year: 2024, Volume and Issue: 79(9), P. 1167 - 1187
Published: Sept. 1, 2024
Language: Английский
Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 129 - 129
Published: Jan. 23, 2025
This research aimed to evaluate block freeze concentration (BFC) under different centrifugation conditions using response surface methodology separate an extract from the ice fraction at three centrifugal-BFC (CBFC) cycles, obtaining in final cycle a phenolic-rich extract. A Box–Behnken design was applied optimize variables, with efficiency of separation (η) selected as variable. The extracts were characterized terms physicochemical analysis, total and individual bioactive components, antioxidant capacity. Optimal (3600 rpm, 16 °C, 14 min) resulted η 82%. Thus, infusion cycle, solids, polyphenol flavonoid contents, capacity exhibited 1.81 6.5% (w/w) 2.5 8.7 (°Brix), 0.72 12.2 mg gallic acid equivalents/mL, 0.83 13.7 catequin equivalents /mL, 2.8 31.2 μmol trolox equivalents/mL 4.8 122.2 identifying by high-performance liquid chromatography that kaempferol, p-hydroxybenzoic, transferulic presented highest concentrations. CBFC process has potential non-thermal preserve many compounds, facilitating production concentrated fractions high biological value, where obtained BFC are novel solution for medicinal, pharmaceutical, food applications.
Language: Английский
Citations
2
The Journal of Supercritical Fluids, Journal Year: 2024, Volume and Issue: 207, P. 106211 - 106211
Published: Feb. 8, 2024
This study explored supercritical fluid extraction (SFE) of bioactive molecules from Ammodaucus leucotrichus seeds, a little-known source potent compounds. Response surface methodology assessed parameters (pressure, temperature, and ethanol co-solvent) their effects on global yield, total phenolic compound (TPC), carbohydrate content (TCC), anti-Alzheimer activity. Optimization via Box-Behnken design identified the optimal conditions: 15% ethanol, 300 bar, 70°C. Chemical profiling using GC-MS UHPLC-q-tof-MS/MS detected over 41 metabolites. In addition, biorefinery sequential process was performed to obtain extracts with high neuroprotective potential. The SFE exhibited modest AChE inhibition. Notably, PLE extractions yielded enhanced antioxidant activity compared separate processes. conclusion, presents promising natural secondary Its potential health benefits warrant further exploration, emphasizing need for continued research.
Language: Английский
Citations
12
PeerJ Analytical Chemistry, Journal Year: 2024, Volume and Issue: 6, P. e32 - e32
Published: April 4, 2024
Natural deep eutectic solvents (NADES) have emerged as an eco-friendly alternative for extracting bioactives, avoiding the use of flammable organic and extreme temperatures pH conditions. NADES rely on intermolecular interactions between hydrogen bonding donors (HBD) acceptors (HBA) to form mixtures with significantly lower melting points than their individual components. These matrices are influenced by factors like water content, temperature, component ratios. high viscosity can hinder extractive efficiency, which be mitigated adding or working at higher temperatures. However, excessive dilution may disrupt supramolecular structure NADES, reducing extraction efficiency. A notable feature is fine-tunability specific purposes. Adjusting physicochemical properties such polarity, pH, optimizes efficiency promoting solubility target molecules molecules. unlike solvents, partially plant microalgae cell walls, enhancing permeability Moreover, a stabilising effect bioactives enhance biological activity bioavailability. attributes, coupled low environmental impact in terms toxicity biodegradability, make attractive biorefinery applications.
Language: Английский
Citations
12
Cancers, Journal Year: 2025, Volume and Issue: 17(2), P. 318 - 318
Published: Jan. 20, 2025
Algae are a rich source of bioactive compounds that have wide range beneficial effects on human health and can show significant potential in the treatment hematological malignancies such as leukemia, lymphoma, multiple myeloma. These diseases often pose therapeutic challenge despite recent advances (e.g., use immunomodulatory drugs, proteasome inhibitors, CD38 monoclonal antibodies, stem cell transplant, targeted therapy). A considerable number patients experience relapses or resistance to applied therapies. Algal compounds, alone combination with chemotherapy other more advanced therapies, exhibited antitumor preclinical studies may improve disease outcomes. include ability induce apoptosis, inhibit tumor growth, immune responses. However, most these conducted vitro, without vivo validation clinical trials. This paper summarizes current evidence vitro algae extracts isolated myeloma lines. In addition, we address application algae-derived drug carriers their synergistic against hematologic malignancies.
Language: Английский
Citations
1
Circular Economy and Sustainability, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Language: Английский
Citations
1
Separation and Purification Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131301 - 131301
Published: Dec. 1, 2024
Language: Английский
Citations
6
Advances in Sample Preparation, Journal Year: 2025, Volume and Issue: 13, P. 100149 - 100149
Published: Jan. 16, 2025
Language: Английский
Citations
0
Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: 12, P. 100216 - 100216
Published: Jan. 31, 2025
Language: Английский
Citations
0
Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1754, P. 466030 - 466030
Published: May 8, 2025
Grape pomace is an important by-product generated during winemaking that still possesses relevant concentrations of interesting bioactive compounds. As the production this very high, developing strategies to produce its valorization in effort provide means for generation high-added value products desired. Tannat grapes are particularly rich phenolic compounds and, consequently, derived from them may be those components. The use compressed fluids-based extraction techniques, such as supercritical fluid (SFE) and pressurized liquids (PLE) retains good potential isolation natural matrices. In work, PLE explored obtain extracts grape presenting high antioxidant anticholinergic activities. After optimization, best conditions implied 55 % ethanol a solvent at 141 °C 20 min. Moreover, correlate observed bioactivities with particular chemical composition extracts, new two-dimensional comprehensive liquid chromatography (LC × LC) method coupled mass spectrometry (MS) was developed separate identify components present extract. LC analysis optimum extract separated >70 different analytes. Among them, proanthocyanidins were most abundant compounds, although others flavonols anthocyanins could also identified. Overall, obtained results demonstrate strong valorized, retaining excellent possibilities application fields.
Language: Английский
Citations
0
Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 216, P. 118717 - 118717
Published: May 21, 2024
Ruta graveolens L. is considered an important source of alkaloids and furanocoumarins, with attributed biological activity against phytopathogenic microorganisms. Pressurized liquid extraction (PLE) supercritical fluid (SFE) using bio-based solvents are green alternative techniques to obtain specific type compounds. From aerial parts, terpenes were favorably extracted by PLE, while SFE batch extraction, different fractions obtained depending on the pressure: at 200 bar, fatty acids enrichment was achieved from dry sample, 350 bar mainly furanocoumarins moistened sample. Coumarins roots two tested. However, this family always together furanocoumarins. Therefore, in work a methodology has been developed rue extracts enriched families biologically active substances that could have antifungal or nematicidal for pathogen control agricultural activities.
Language: Английский
Citations
3