DNA-Assisted CRISPR-Cas12a Enhanced Fluorescent Assay for Protein Detection in Complicated Matrices DOI
Sathishkumar Munusamy, Haiyan Zheng,

Rana Jahani

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Proteins are important biological macromolecules that perform a wide variety of functions in the cell and human body, can serve as biomarkers for early diagnosis prognosis diseases well monitoring effectiveness disease treatment. Hence, sensitive accurate detection proteins biospecimens is imperative. However, at present, there no ideal method available clinical samples, many which present ultralow (less than 1 pM) concentrations complicated matrices. Herein, we report an ultrasensitive selective DNA-assisted CRISPR-Cas12a enhanced fluorescent assay (DACEA) protein with limits reaching low attomolar concentrations. The high sensitivity was accomplished through combined DNA barcode amplification (by using dual-functionalized AuNPs) CRISPR analysis, while selectivity resistance to matrix effects our were via formation protein-antibody sandwich structure specific recognition Cas12a (under guidance crRNA) toward designed target ssDNA. Given its ability accurately sensitively detect trace amounts matrices, DACEA platform pioneered this work has potential application routine biomarker testing.

Language: Английский

Hairpin reporter facilitated single Cas13a test for amplification-free highly sensitive detection of miRNA-424 DOI

Mohammed Z.A.A. AL-Dulaiei,

Tao Ma,

Jinyan Long

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113768 - 113768

Published: April 1, 2025

Language: Английский

Citations

0

CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety DOI
Jacob Tizhe Liberty, Sabri Bromage,

Endurance Peter

et al.

Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0

A review of deoxyribonucleic acid-based single-nucleotide polymorphisms in diabetic kidney disease among Asian populations: Challenges and future directions DOI

C.L. Chopra,

Deepak Kukkar, Poornima Bhatt

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144407 - 144407

Published: May 1, 2025

Language: Английский

Citations

0

Dual Detection of Hepatitis B and C Viruses Using CRISPR‐Cas Systems and Lateral Flow Assay DOI Creative Commons
Syeda Najidah Shahni, Sarah Albogami, Iqbal Azmi

et al.

Journal of Nucleic Acids, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow assay (LFA) HCV, demonstrating concentration‐dependent signal increase. Importantly, no cross‐reactivity was observed with other viral targets. To further enhance sensitivity, employed dual‐enzyme approach, combining Cas12 Cas13 in single reaction, which significantly improved limits viruses. Finally, dual antigen LFA strip capable simultaneously detecting sample. This approach holds promise point‐of‐care (POC) diagnostics where infection is unknown. study addresses current limitations CRISPR‐Cas based diagnostics, namely, need ultrasensitive methods ability to detect antigens using test strip. Our findings demonstrate feasibility highly paving way potential POC application.

Language: Английский

Citations

3

Development of an On-Site Real-Time Dual Detection Method for Norovirus and Rotavirus Using RPA-CRISPR/Cas12,13 DOI
X. Chris Le,

Jing Jiang,

Hong Yi

et al.

Food Control, Journal Year: 2024, Volume and Issue: unknown, P. 110943 - 110943

Published: Oct. 1, 2024

Language: Английский

Citations

3

CRISPR/Cas12a powered sandwich aptamer assay coupled with effective DNA self-assembly amplification for sensitive detection of immunoglobulin E DOI

Fengxi Zhu,

Qiang Zhao

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136615 - 136615

Published: Sept. 10, 2024

Language: Английский

Citations

0

Advances in Helicobacter pylori detection technology: From pathology-based to multi-omic based methods DOI

Huishu Huang,

Minghao Yin,

Yuwei Wu

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 182, P. 118041 - 118041

Published: Nov. 10, 2024

Language: Английский

Citations

0

DNA-Assisted CRISPR-Cas12a Enhanced Fluorescent Assay for Protein Detection in Complicated Matrices DOI
Sathishkumar Munusamy, Haiyan Zheng,

Rana Jahani

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Proteins are important biological macromolecules that perform a wide variety of functions in the cell and human body, can serve as biomarkers for early diagnosis prognosis diseases well monitoring effectiveness disease treatment. Hence, sensitive accurate detection proteins biospecimens is imperative. However, at present, there no ideal method available clinical samples, many which present ultralow (less than 1 pM) concentrations complicated matrices. Herein, we report an ultrasensitive selective DNA-assisted CRISPR-Cas12a enhanced fluorescent assay (DACEA) protein with limits reaching low attomolar concentrations. The high sensitivity was accomplished through combined DNA barcode amplification (by using dual-functionalized AuNPs) CRISPR analysis, while selectivity resistance to matrix effects our were via formation protein-antibody sandwich structure specific recognition Cas12a (under guidance crRNA) toward designed target ssDNA. Given its ability accurately sensitively detect trace amounts matrices, DACEA platform pioneered this work has potential application routine biomarker testing.

Language: Английский

Citations

0