ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Prion
diseases
are
fatal
neurodegenerative
disorders
caused
by
the
misfolding
and
aggregation
of
cellular
prion
protein
(PrPC)
into
its
pathogenic
form
(PrPSc),
leading
to
progressive
neurodegeneration.
Currently,
no
effective
treatments
available,
highlighting
need
for
novel
therapeutic
strategies.
In
this
study,
we
explored
potential
Moringa
oleifera
extracts
as
a
source
bioactive
compounds
that
could
modulate
aggregation.
A
hydroethanolic
extract
from
M.
leaves
was
analyzed
using
PrP
inhibition
profiling
via
real-time
quaking-induced
conversion
(RT-QuIC)
assays,
in
combination
with
affinity
selection-mass
spectrometry
(AS-MS).
This
approach
identified
chlorogenic
neochlorogenic
acids
potent
inhibitors
These
exhibited
significant
antiprion
activity,
IC50
values
64.41
±
12.12
35.34
7.09
μM,
respectively.
addition
inhibiting
PrPC
PrPSc,
both
disaggregate
preformed
PrPSc
fibrils
vitro.
AS-MS
proved
be
valuable
tool
isolating
modulators
directly
crude
natural
product
extracts,
avoiding
expensive
time-consuming
fractionation
purification
processes.
Identifying
highlights
products
combating
other
amyloidogenic
disorders.
Our
findings
suggest
these
serve
promising
lead
developing
diseases.
Further
vivo
studies
pharmacokinetic
optimization
warranted
explore
their
full
potential.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 6, 2025
The
chemodiversity
of
plants
is
a
valuable
resource
for
drug
discovery,
and
its
combination
with
modern
approaches
can
reduce
the
time
consumption
bioactive
metabolite
discovery.
This
study
aimed
to
evaluate
chemical
constituents
from
18
plant
species
different
families
against
leukemia
cancer
cells
application
statistical
analysis
metabolomic
data
molecular
networking
prediction
metabolites.
samples,
extracted
by
an
accelerated
solvent
extractor
using
ethanol
water
7:3
(v/v),
were
analyzed
LC-DAD-MS
evaluated
(Kasumi-1,
KG-1,
K-562).
Chemical
aligned,
statistics,
applied
create
network.
Sesbania
virgata,
Aeschynomene
denticulata,
Erythroxylum
angiufugum,
Psidium
guineense,
Astronium
fraxinifolium,
Coccoloba
ochreolata,
Solanum
glaucophyllum
(S.
glaucophyllum),
Paullinia
pinnata
inhibited
K-562
cell
viability
approximately
70%
at
100
μg/mL,
while
Ocotea
diospyrifolia
showed
35%
inhibition
KG-1
lineage.
Alkaloid
fractions
S.
O.
revealed
EC50
values
ranging
13.9
6.4
μg/mL
lines,
effectively
inducing
death
apoptotic
characteristics,
membrane
integrity
loss,
signs
late
apoptosis.
was
essential
crucial
complement
analysis,
which
performed
430
features
targeted
steroidal
aporphine
alkaloids.
Boldine
46,
116,
145
μM
Kasumi,
respectively.
findings
marked
relevance
broader
predict
compounds,
emphasizing
potential
benefits
in
search
metabolites
cells,
particularly
Chemical and Pharmaceutical Bulletin,
Journal Year:
2025,
Volume and Issue:
73(3), P. 189 - 194
Published: March 6, 2025
Six
flavonoids
(1-6),
including
3
previously
undescribed
compounds
(1-3),
were
isolated
from
the
dried
roots
and
stem
skins
of
Daphne
giraldii
Nitsche.
The
strategy
LC-tandem
mass
spectrometry-based
Global
Natural
Products
Social
Molecular
Networking
(GNPS)
molecular
network
technology
NMR-based
Small
Molecule
Accurate
Recognition
Technology
(SMART)
facilitated
precise
separation
isopentenyl
in
D.
giraldii.
structures
determined
through
comprehensive
spectroscopic
analysis.
Furthermore,
all
evaluated
for
their
cytotoxic
activity
against
Hep3B
cells.
Specifically,
1
exhibited
significant
cytotoxicity
with
IC50
values
5.52
±
0.57
2.53
0.49
μM,
respectively,
compared
to
positive
control
sorafenib
(IC50
=
7.08
0.23
μM).
