Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
73(2), P. 1598 - 1607
Published: Dec. 31, 2024
In
this
study,
we
present
an
intelligent
electromagnetic-actuated
microfluidic
chip
integrated
with
a
G-quadruplex
DNAzyme-based
biocatalysis
platform
for
rapid
and
sensitive
tetracycline
(TC)
detection.
sensing
system,
TC
significantly
quenches
fluorescent
magnetic
carbon
dots
(M-CDs)
via
the
internal
filtration
effect
dynamic
quenching
(the
excitation
emission
wavelength
at
350
440
nm,
respectively).
Then,
on
M-CDs-Aptamer
is
exposed
bound
hemin
to
form
hemin-G-quadruplex
DNAzyme,
catalyzing
conversion
of
3,3',5,5'-tetramethylbenzidine
produce
blue
color.
This
enables
fluorescence/colorimetric
detection
TC.
Importantly,
automatic
electromagnet-integrated
was
designed
control
shuttling
materials
in
each
function
slot
according
programmed
sequence.
Under
optimal
conditions,
limits
fluorescence
colorimetric
methods
were
11
43
μmol/L,
respectively.
The
results
tilapia
(Oreochromis
nilotica)
comparable
those
traditional
high-performance
liquid
chromatography.
offers
excellent
performance
determination
potential
portable,
trace
pollutants
food
environment.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
73(2), P. 1598 - 1607
Published: Dec. 31, 2024
In
this
study,
we
present
an
intelligent
electromagnetic-actuated
microfluidic
chip
integrated
with
a
G-quadruplex
DNAzyme-based
biocatalysis
platform
for
rapid
and
sensitive
tetracycline
(TC)
detection.
sensing
system,
TC
significantly
quenches
fluorescent
magnetic
carbon
dots
(M-CDs)
via
the
internal
filtration
effect
dynamic
quenching
(the
excitation
emission
wavelength
at
350
440
nm,
respectively).
Then,
on
M-CDs-Aptamer
is
exposed
bound
hemin
to
form
hemin-G-quadruplex
DNAzyme,
catalyzing
conversion
of
3,3',5,5'-tetramethylbenzidine
produce
blue
color.
This
enables
fluorescence/colorimetric
detection
TC.
Importantly,
automatic
electromagnet-integrated
was
designed
control
shuttling
materials
in
each
function
slot
according
programmed
sequence.
Under
optimal
conditions,
limits
fluorescence
colorimetric
methods
were
11
43
μmol/L,
respectively.
The
results
tilapia
(Oreochromis
nilotica)
comparable
those
traditional
high-performance
liquid
chromatography.
offers
excellent
performance
determination
potential
portable,
trace
pollutants
food
environment.