Target-Triggered DNA Hydrogel Gating with Nanoconfinement Effect in Micropipettes for Antibiotic Resistance Gene Detection DOI

Xueyan Zhou,

Juan Song, Qi Zhu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

A novel biosensing platform integrating molecular gating mechanisms with confined-space amplification is presented for ultrasensitive nucleic acid detection. The employs a target-triggered clamped hybridization chain reaction (C-HCR) to form DNA hydrogel networks in gold-modified glass micropipettes, creating nanoconfined spaces. Notably, this configuration demonstrates effective ion enhanced sensitivity through the nanoconfinement effect as modulation of current rectification signals significantly amplified. Moreover, can be regenerated thermal treatment, endowing device good recoverability. performance was validated by using different antibiotic resistance genes model targets. results showed high specificity and limits detection down subnanomolar levels. confined environment enables sensitive small sample volumes only 4 μL. strategy leveraging amplify signal transduction may provide inspiration rational design other high-performance biosensors, potentially holding great promise disease diagnostics environmental monitoring.

Language: Английский

Intrinsic Fluorescence Hydrogels for ON/OFF Screening of Antidiabetic Drugs: Assessing α-Glucosidase Inhibition by Acarbose DOI Creative Commons
F. Javier Patiño, Josué M. Galindo, Alicia González Jiménez

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Diabetes remains one of the most prevalent chronic diseases globally, significantly impacting mortality ratetables. The development effective treatments for controlling glucose level in blood is critical to improve quality life patients with diabetes. In this sense, smart optical sensors using hydrogels, responsive external stimuli, have emerged as a revolutionary approach diabetes care. study, changes properties hydrogel are employed monitoring α-glucosidase activity, enzyme involved mellitus type II due its role breaking terminal α-glycosidic bonds, releasing α-glucose. encapsulated within triazine-based that exhibits intrinsic blue fluorescence. Upon hydrolysis substrate p-nitrophenyl-α-D-glucopyranoside (p-NPG) by α-glucosidase, fluorescence quenched release p-nitrophenol (PNP). However, when exposed potential antidiabetic drugs, enzyme's activity inhibited, and hydrogel's intact. This ON/OFF fluorescence-based assay enables rapid screening drug candidates evaluating their ability inhibit enzymatic activity. Sensor optimization involves conducting swelling studies, fluorescent assays, reusability tests trial real drug. innovative holds enhancing management, offering more accessible efficient solution compared traditional biosensors.

Language: Английский

Citations

0
A Novel Resazurin-Loaded Electroactive Hydrogel Enhanced Methane Production in Anaerobic Digestion by Triggering Dual Electron Transfer Pathways and Metabolic Regulation DOI
Cheng Huang, Nan Sun,

Yaqian Chai

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Language: Английский

Citations

0
Target-Triggered DNA Hydrogel Gating with Nanoconfinement Effect in Micropipettes for Antibiotic Resistance Gene Detection DOI

Xueyan Zhou,

Juan Song, Qi Zhu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

A novel biosensing platform integrating molecular gating mechanisms with confined-space amplification is presented for ultrasensitive nucleic acid detection. The employs a target-triggered clamped hybridization chain reaction (C-HCR) to form DNA hydrogel networks in gold-modified glass micropipettes, creating nanoconfined spaces. Notably, this configuration demonstrates effective ion enhanced sensitivity through the nanoconfinement effect as modulation of current rectification signals significantly amplified. Moreover, can be regenerated thermal treatment, endowing device good recoverability. performance was validated by using different antibiotic resistance genes model targets. results showed high specificity and limits detection down subnanomolar levels. confined environment enables sensitive small sample volumes only 4 μL. strategy leveraging amplify signal transduction may provide inspiration rational design other high-performance biosensors, potentially holding great promise disease diagnostics environmental monitoring.

Language: Английский

Citations

0