Adapting the Laser‐Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High‐Sensitivity Detection of 2‐Aminoacridone Labeled Oligosaccharides DOI Creative Commons
Filip Duša, Marcelina Rusin, Denisa Smolkova

et al.

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(3)

Published: March 1, 2025

ABSTRACT The high‐sensitivity capabilities of laser‐induced fluorescence (LIF) detection continuously promote the development various labels with different properties. However, this strategy also requires adaptation existing systems to suit excitation and emission characteristics novel fluorescent tags. In study, we adapted LIF detector commercial capillary electrophoresis instrument specific spectra 2‐aminoacridone labeled human milk oligosaccharides. An external solid‐state laser a wavelength 405 nm was connected via simple 3D‐printed laser‐to‐light‐guide adapter, optical filter setups were compared based on signal‐to‐noise ratio. optimized setup provided limits as low 0.27 0.34 nM, corresponding injection 3.4 4.6 attomoles These findings show that configuration can enhance sensitivity electrophoretic separation by several orders magnitude. addition, presented be utilized guide for coupling lasers instrument.

Language: Английский

Advancements in photoelectrochemical sensors for analysis of food contaminants DOI

Kexin Zou,

Shumin Zhang, Quansheng Chen

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: 157, P. 104903 - 104903

Published: Feb. 6, 2025

Language: Английский

Citations

1
Adapting the Laser‐Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High‐Sensitivity Detection of 2‐Aminoacridone Labeled Oligosaccharides DOI Creative Commons
Filip Duša, Marcelina Rusin, Denisa Smolkova

et al.

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(3)

Published: March 1, 2025

ABSTRACT The high‐sensitivity capabilities of laser‐induced fluorescence (LIF) detection continuously promote the development various labels with different properties. However, this strategy also requires adaptation existing systems to suit excitation and emission characteristics novel fluorescent tags. In study, we adapted LIF detector commercial capillary electrophoresis instrument specific spectra 2‐aminoacridone labeled human milk oligosaccharides. An external solid‐state laser a wavelength 405 nm was connected via simple 3D‐printed laser‐to‐light‐guide adapter, optical filter setups were compared based on signal‐to‐noise ratio. optimized setup provided limits as low 0.27 0.34 nM, corresponding injection 3.4 4.6 attomoles These findings show that configuration can enhance sensitivity electrophoretic separation by several orders magnitude. addition, presented be utilized guide for coupling lasers instrument.

Language: Английский

Citations

0