SERS-Based Immunochromatographic Assay for Sensitive Detection of Escherichia coli O157:H7 Using a Novel WS2-AuDTNB Nanotag

Sensors, Journal Year: 2025, Volume and Issue: 25(8), P. 2457 - 2457

Published: April 14, 2025

E. coli O157:H7 contamination in food and the environment poses a serious threat to human health. Rapid sensitive identification of foodborne pathogens remains challenging. Here, we prepared tungsten disulfide (WS2)–Au nanocomposites coupled with Raman signal molecule 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) antibodies replace conventional colloidal gold nanoparticles applied SERS-active nanotags SERS-ICA method for highly detection O157:H7. The large surface area numerous effective SERS hotspots WS2-Au provide superior signals. Under optimized conditions, this ICA achieves quantitative broad linear range 8 × 102–8 107 CFU/mL at low limit 175 CFU/mL. In addition, test strip indicates high specificity identification, favorable reproducibility, shows good accuracy actual samples, such as milk pork. proposed assay can be used rapid qualitative has great potential field application.

Language: Английский

Two lateral flow immunoassay assays based on different nanoparticle probes for the on-site detection of Streptococcus pyogenes in raw milk

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113689 - 113689

Published: April 1, 2025

Language: Английский

A review on enzymatic colorimetric assays for organophosphate and carbamate pesticides detection in water environments

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106423 - 106423

Published: April 1, 2025

Language: Английский