Missense mutations of GPER1 in breast invasive carcinoma: Exploring gene expression, signal transduction and immune cell infiltration with insights from cellular pharmacology DOI Open Access
Yu Zhang, Chong Du, Shu-Qun Zhang

et al.

Biomedical Reports, Journal Year: 2024, Volume and Issue: 22(2)

Published: Nov. 29, 2024

G protein‑coupled estrogen receptor 1 (GPER1) plays a crucial role in the progression of breast cancer and has emerged as promising therapeutic target. However, while missense mutations GPER1 have been detected invasive carcinoma (BIC) samples, resulting molecular, cellular pharmacological changes remain unclear. The present study categorized BIC samples from Cancer Genome Atlas database based on mutation information available cBioPortal database. Subsequently, survival analysis was conducted screened for differentially expressed genes (DEGs). Using these DEGs, performed Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses, protein‑protein interaction network hub gene selection. After assessing prognostic value genes, immune cell infiltration between mutant wild‑type (WT) groups analyzed. Finally, luciferase reporter system used to assess cyclic AMP (cAMP) production mediated by following treatment with agonist G‑1 each mutation. results revealed significant decrease progression‑free disease‑specific group compared WT group. expression identified 60 all which were upregulated significantly enriched GO terms related tumor progression, such organic anion transport, glycosaminoglycan binding monoatomic ion‑gated channel activity. DEGs also PI3K‑Akt signaling pathway KEGG. Hub selection evaluation three associated survival: IL33, STAB2 CFTR. Immune CD8 T content Luciferase assays demonstrated that four (L129M, E218Q, S235F A345G) attenuated induction adenosine monophosphate its agonist. These findings provided valuable insights design drugs targeting precision medicine initiatives.

Language: Английский

Intratumoral microbiota, fatty acid metabolism, and tumor microenvironment constitute an unresolved trinity in colon adenocarcinoma DOI Creative Commons

G Liu,

K. Liu, Lei Ji

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 20, 2025

The intratumoral microbiota, fatty acid metabolism (FAM), and tumor microenvironment (TME) all provide insights into the management of colon adenocarcinoma (COAD). But biological link among three remains unclear. Here, we analyzed microbiome samples matched host transcriptome from 420 patients with COAD in Cancer Genome Atlas (TCGA). All were divided two subtypes (FAM_high FAM_low) based on Gene set variation analysis (GSVA) score FAM pathway. Furthermore, found significant difference microbiota signatures between subtypes. In-depth suggested that specific microbes tumors may indirectly modify TME, particularly stromal cell populations, by modulating process. More importantly, crosstalk can have a impact prognosis, response to immunotherapy, drug sensitivity patients. Pathological image profiling showed changes TME originating disturbance could be reflected pathological features. In summary, our study provides novel links FAM, COAD, offer guidance for therapeutic opportunities target microbes.

Language: Английский

Citations

0
Pan-Cancer Analysis and Validation of NT5DC2: Emphasizing Its Prognostic Significance and Immunological Role in TNBC DOI Creative Commons
Qing Luo, Xianjun Xie, Luyao Tian

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Background 5′-Nucleotidase Domain Containing 2 (NT5DC2), a cNT5-II family member catalyzing nucleotide hydrolysis, plays crucial role in tumor initiation and progression. This study aims to elucidate NT5DC2’s potential multiple cancers confirm its oncogenic significance triple-negative breast cancer (TNBC). Methods Multiple databases analyzed NT5DC2 expression patterns assessed diagnostic prognostic value cancers. Immune correlation analyses were conducted using ESTIMATE CIBERSORT. KEGG pathway enrichment analysis explored NT5DC2-associated molecular pathways. To further investigate TNBC, comprehensive bioinformatics analyses, including gene profiling, single-cell RNA sequencing analysis, immune infiltration assessment, set (GSEA). Finally, in vitro experiments validate TNBC. Results Our findings indicate that high is associated with poor prognosis holds significant clinical across types. highly expressed TNBC correlates unfavorable outcomes. Single-cell reveals predominantly epithelial cells, where it regulates cells through the MIF signaling pathway. Enrichment closely linked an immunosuppressive microenvironment. In demonstrate knockdown significantly inhibits cell growth, underscoring as therapeutic target. Conclusion demonstrates functions oncogene It considerable intricately microenvironment, particularly

Language: Английский

Citations

0
IGF2BP3/CTCF Axis–Dependent NT5DC2 Promotes M2 Macrophage Polarization to Enhance the Malignant Progression of Lung Squamous Cell Carcinomas DOI Creative Commons

Jifeng Sun,

Hao Wang, Ran Zhang

et al.

The Clinical Respiratory Journal, Journal Year: 2024, Volume and Issue: 18(11)

Published: Nov. 1, 2024

ABSTRACT Background Lung squamous cell carcinoma (LUSC) is a type of lung cancer that develops in the cells. It known to be promoted by activation various signaling pathways and dysregulation key regulatory molecules. One such molecule, 5′‐nucleotidase domain containing 2 (NT5DC2), has been identified as critical regulator cancers including cancer. However, there are no data regarding its role LUSC. Methods The mRNA expression insulin‐like growth factor mRNA–binding protein 3 (IGF2BP3), CCCTC‐binding (CTCF), NT5DC2 was analyzed using quantitative real‐time polymerase chain reaction (qRT‐PCR), whereas their assessed western blotting assay. Cell proliferation determined counting kit‐8 (CCK‐8) apoptosis, CD11b expression, CD206 were flow cytometry. Tube formation through tube Glucose consumption, lactate production, ATP levels measured colorimetric methods. effect on malignant progression LUSC cells xenograft mouse model transforming factor‐beta 1 (TGF‐β1) interleukin‐10 (IL‐10) detected enzyme‐linked immunosorbent assays. associations among IGF2BP3, CTCF dual‐luciferase reporter assay, RNA immunoprecipitation assay m6A Results found upregulated tissues when compared with normal human bronchial epithelial Silencing inhibited proliferation, formation, glycolysis, M2 macrophage polarization, tumor while inducing apoptosis. In addition, transcriptionally activate IGF2BP3 stabilized methylation. Further, overexpression or attenuated effects silencing both NCI‐520 SK‐MES‐1 Conclusion IGF2BP3/CTCF axis–dependent promotes thereby enhancing This study first reveal underlying mechanism. result suggests targeting IGF2BP3/CTCF/NT5DC2 axis may have clinical significance treatment

Language: Английский

Citations

1
Identification of key modules and hub genes involved in regulating the feather follicle development of Wannan chickens using WGCNA DOI Creative Commons

Jiangxian Wang,

Chaohui Xing,

Hao Wang

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(8), P. 103903 - 103903

Published: May 25, 2024

Carcass appearance is important economic trait, which affects customers in making purchase decisions. Both density and diameter of feather follicles are two indicators carcass appearance. However, the regulatory network key genes be involved follicle development remain poorly understood. To identify modules that chickens, 16 transcriptome datasets Wannan chickens skin tissue (3 birds at E9, E11, E14, respectively, 7 12W) were used for weighted gene co-expression analysis (WGCNA) analysis, 12 samples each stage) selected DEGs analysis. A total 5,025, 2,337, 10,623 identified 3 comparison groups, including E9 vs. E11 E14 12W. Additionally, 31 by WGCNA dark-orange, cyan, blue module found to significantly associated with (p < 0.01). In total, 92,898 8,448 hub obtained modules, respectively. We focused on cyan as 6 336 these overlap three The overlapped such LAMC2, COL6A3, COL6A2 etc., over-represented categories focal adhesion ECM-receptor interaction signaling pathway. Among between different groups several WNT7A WNT9B enriched Wnt These results suggested COL6A2, WNT7A, may play a crucial role regulation chickens. Our provided reference molecular contribute breeding traits

Language: Английский

Citations

0
Missense mutations of GPER1 in breast invasive carcinoma: Exploring gene expression, signal transduction and immune cell infiltration with insights from cellular pharmacology DOI Open Access
Yu Zhang, Chong Du, Shu-Qun Zhang

et al.

Biomedical Reports, Journal Year: 2024, Volume and Issue: 22(2)

Published: Nov. 29, 2024

G protein‑coupled estrogen receptor 1 (GPER1) plays a crucial role in the progression of breast cancer and has emerged as promising therapeutic target. However, while missense mutations GPER1 have been detected invasive carcinoma (BIC) samples, resulting molecular, cellular pharmacological changes remain unclear. The present study categorized BIC samples from Cancer Genome Atlas database based on mutation information available cBioPortal database. Subsequently, survival analysis was conducted screened for differentially expressed genes (DEGs). Using these DEGs, performed Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses, protein‑protein interaction network hub gene selection. After assessing prognostic value genes, immune cell infiltration between mutant wild‑type (WT) groups analyzed. Finally, luciferase reporter system used to assess cyclic AMP (cAMP) production mediated by following treatment with agonist G‑1 each mutation. results revealed significant decrease progression‑free disease‑specific group compared WT group. expression identified 60 all which were upregulated significantly enriched GO terms related tumor progression, such organic anion transport, glycosaminoglycan binding monoatomic ion‑gated channel activity. DEGs also PI3K‑Akt signaling pathway KEGG. Hub selection evaluation three associated survival: IL33, STAB2 CFTR. Immune CD8 T content Luciferase assays demonstrated that four (L129M, E218Q, S235F A345G) attenuated induction adenosine monophosphate its agonist. These findings provided valuable insights design drugs targeting precision medicine initiatives.

Language: Английский

Citations

0