Strain‐dependent and host genotype–dependent priority effects in gut microbiome assembly affect host fitness in Daphnia

Limnology and Oceanography, Journal Year: 2024, Volume and Issue: 69(8), P. 1782 - 1796

Published: July 8, 2024

Abstract The arrival order of species can strongly influence the early assembly ecological communities. Such events, known as priority effects, are documented in various settings, but remain understudied within context gut microbiome. Given fundamental link between microbiome and host health, exploring potential role effects shaping development microbial communities becomes imperative. Using freshwater planktonic crustacean Daphnia magna a model system, we manipulated immigration three bacterial strain pairs two germ‐free genotypes quantified composition fitness at time points, namely day 8 12. Priority were observed; however, their presence, amplitude, direction (suppressive or facilitative) found to be contingent on identity genotype. These findings accompanied by notable differences life history traits across inoculation treatments, shedding light tangible consequences triggered sequence environment, for fitness. Our results thus highlight complex nature community assembly, strain/genotype specificity, impact host.

Language: Английский

---

The Emergence of the Slc11 Clade MCbgut: A Parsimonious Hypothesis for the Dawn of Lactobacillales in the Gut of Early Vertebrates

Bacteria, Journal Year: 2024, Volume and Issue: 3(3), P. 223 - 255

Published: Aug. 14, 2024

The Lactobacillales (LB) stand apart among bacterial orders, using manganese (Mn) instead of iron to support their growth and swiftly ferment complex foods while acidifying environment. present work investigates whether a shift in the use Mn could mark origin LB. Transmembrane carriers ubiquitous Slc11 family play key roles LB physiology by catalyzing proton-dependent import. In prior studies, clade found (MntH Cb, MCb) showed both remarkable structural plasticity highly efficient uptake, another clade, MCg1, demonstrated divergent evolution coinciding with emergence genera (e.g., Bordetella, Achromobacter). Herein, MCb is subdivided sister groups: MCbie MCbgut. derives directly from MCa, pointing an intermediate stage predominates marine Bacillaceae, more conserved than MCbgut, lacks that typify MCbgut carriers, responds differently identical mutagenesis. Exchanging MCbie/MCbgut amino acid residues at sites distinguish these clades conformation-dependent effects templates, 3D location targeted carrier structure together suggests mechanism open inner gate, release into cytoplasm, differs between Building on established phylogeny for Enterococcus revealed pair genes encoding was common ancestor LB, as MCbgu1 MCbgu2 templates exhibited distinct dynamics properties. These data are discussed when examining MCbgut+ emerge upper gut early vertebrates (ca. 540 mya), through genome contraction toward Mn-centrism, they specialized gastric aids favoring stomach establishment jawed bi-directional communication host nervous, endocrine immune systems.

Language: Английский

---

How does co-occurrence ofDaphniaspecies affect their gut microbiome?

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

Abstract Species co-occurrence can lead to competitive interactions that influence fitness. Competition is typically assumed be modulated by species niche, especially food–acquisition related traits. The of interspecific on host microbiome communities has rarely been considered, and yet may provide an alternative mechanism regarding the effect their Here, we investigated whether composition gut microbial community differs between two Daphnia ( D. magna pulex ), one depends presence other. We hypothesized stronger filter-feeder have a larger weaker than vice versa . To this purpose, three genotypes were first made axenic then grown in monocultures or cocultures natural environmental bacterioplankton-enriched water, before assessing microbiomes bacterioplankton. found did not significantly differ overall. However, subtle differences mono- at genotype level. For most combinations (six out nine), changed more when with monocultures. This provides limited support for our hypothesis competitor , possibly mediated via bacterioplankton community.

Language: Английский

---

How does co‐occurrence of Daphnia species affect their gut microbiome?

Oikos, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Species co‐occurrence can lead to competitive interactions that influence fitness. Competition is typically assumed be modulated by species niche, especially food‐acquisition related traits. The of interspecific on host microbiome communities has rarely been considered, and yet may provide an alternative mechanism regarding the effect their Here, we investigated whether composition gut microbial community differs between two Daphnia ( D. magna pulex ), one depends presence other. We hypothesized stronger filter‐feeder have a larger weaker than vice versa. To this purpose, three genotypes were first made axenic then grown in monocultures or co‐cultures natural environmental bacterioplankton‐enriched water, before assessing microbiomes bacterioplankton. found did not significantly differ overall. However, subtle differences (i.e. relative abundance certain bacteria) mono‐ at genotype level. For most combinations (six out nine), changed more distance was sensitive culture type ) when with monocultures. This provides limited support for our hypothesis versa, possibly mediated via bacterioplankton community.

Language: Английский

---