Molecules,
Journal Year:
2024,
Volume and Issue:
29(19), P. 4560 - 4560
Published: Sept. 25, 2024
Cordyceps
militaris
solid
medium
polysaccharides
(CMMPs)
were
extracted
using
an
ultrasound-assisted
enzyme
method,
and
the
process
conditions
optimized
via
response
surface
methodology
(RSM).
The
CMMPs
separated
into
four
components
named
CMMP−1,
CMMP−2,
CMMP−3
CMMP−4
ethanol
fractional
precipitation,
their
monosaccharide
composition
structural
properties
analyzed
by
molecular
weight
analysis,
Fourier-transform
infrared
spectroscopy
(FT–IR),
scanning
electron
microscopy
(SEM),
Congo
red
test,
ultraviolet–visible
(UV-vis),
atomic
force
(AFM),
thermogravimetric
analysis
(TGA).
RSM
could
predict
yield
of
CMMP
(R2
=
0.9928),
polysaccharide
was
15.43%
under
selected
3.1%
cellulase
addition,
a
liquid–solid
ratio
42:1,
extraction
temperature
61
°C,
time
60
min.
Glucose
galactose
main
constituents
precipitated
polysaccharides.
Furthermore,
exhibited
antioxidant
activity,
CMMP−1
demonstrated
stronger
activity
in
vitro.
This
study
demonstrates
possibility
developing
natural
food
from
medium.
Ultrasonics Sonochemistry,
Journal Year:
2024,
Volume and Issue:
108, P. 106948 - 106948
Published: June 12, 2024
In
this
study,
the
process
conditions,
physicochemical
properties,
structural
composition
and
activity
of
polysaccharides
isolated
from
leechee
peel
(LPP)
by
ultrasound-assisted
extraction
(UAE)
with
enzyme
alkali
solution
(ASE)
were
compared.
The
results
showed
that
total
sugar
content
LPP
extracted
UAE
accounted
for
75.65
%,
which
was
significantly
higher
than
solution.
optimum
conditions
as
follows:
temperature
68.78
℃,
ultrasonic
enzymolysis
time
39.68
min,
pectinase
dosage
4.03
solid–liquid
ratio
1:30
g/mL,
power
360
W.
antioxidant
activities
structure
polysaccharide
prepared
under
different
It
found
UAE-LPP
an
α-type
containing
15.83
%
uronic
acid.
Moreover,
strong
in
anti-lipid
peroxidation
reducing
ability.
Ultrasound-assisted
enzymatic
method
is
effective
means
to
improve
natural
plant
polysaccharides,
has
advantages
short
time-consuming,
simple
easy
operation,
can
greatly
utilization
rate
lay
a
theoretical
scientific
basis
development
LPP.
Food & Function,
Journal Year:
2024,
Volume and Issue:
15(8), P. 3939 - 3958
Published: Jan. 1, 2024
Extraction,
purification,
structural
modification,
activities
and
application
of
polysaccharides
from
different
parts
mulberry
were
analyzed
discussed.
Ultrasonics Sonochemistry,
Journal Year:
2023,
Volume and Issue:
96, P. 106420 - 106420
Published: April 28, 2023
Ipomoea
batatas
polysaccharides
(IBPs)
have
many
important
physiological
functions.
The
optimal
extraction
conditions
were
time
of
40
min,
solid-liquid
ratio
1:8
and
ultrasonic
power
240
W.
1D/2D
nuclear
magnetic
resonance
(1D/2D
NMR)
analysis
showed
that
the
main
chain
IBP-1A
was
mainly
composed
→4)-α-D-Glcp-(1→
→3,
6)-β-D-Glcp-(1→
residues.
In
vivo
experiments
polysaccharide
significantly
increased
levels
antioxidation-related
enzymes
metabolites
in
older
mice.
It
could
relieve
oxidative
stress
injury
delay
aging.
Therefore,
this
study
provided
a
new
theoretical
basis
for
development
IBPs
as
antioxidant
food.
Ultrasonics Sonochemistry,
Journal Year:
2023,
Volume and Issue:
99, P. 106571 - 106571
Published: Sept. 1, 2023
Response
surface
methodology
was
selected
to
explore
the
ultrasonic-assisted
cellulase
extraction
conditions
of
Garcinia
mangostana
rind
polysaccharides
(GMRPs),
and
optimum
values
each
condition
were
as
follows:
ratio
raw
material
liquid
1:50
g/mL,
ultrasonic
time
40
min,
enzyme
concentration
4
%,
power
179
W.
Based
on
above
conditions,
average
rate
GMRPs
15.56
%.
modified
by
carboxymethylation,
relationship
between
amount
chloroacetic
acid
substitution
degree
carboxymethylated
derivative
compared.
results
single
factor
experiment,
it
shown
that
significantly
affected
products.
The
research
provides
some
valuable
theoretical
references
for
preparation
its
carboxymethylation
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(2), P. 149 - 149
Published: Jan. 27, 2025
Coriander
(Coriandrum
sativum)
is
a
classical
medicinal
and
edible
herb
as
well
spice,
but
the
physicochemical
biological
properties
of
its
polysaccharides
have
not
been
fully
studied.
In
this
study,
were
extracted
using
an
ultrasonic-assisted
method
purified
from
fresh
coriander,
then
coriander
polysaccharide
(CSP)
fraction
was
separated
agarose
gel
Q-Sepharose
Fast
Flow
column.
The
total
sugar
content,
protein
content
monosaccharides
composition
CSPs
determined
phenol–sulfuric
acid
method,
Coomassie
Brilliant
Blue
HPLC.
structural
characterization
detected
ultraviolet
spectrophotometry
FT-IR
spectroscopy.
DPPH
ABTS
free
radicals
used
to
explore
their
antioxidant
activities,
while
inhibitory
abilities
α-amylase
α-glucosidase
evaluate
hypoglycemic
activity.
After
that,
immunomodulatory
antitumor
activities
investigated
macrophage
RAW264.7
HepG2
cells
targets.
results
showed
that
contents
66.90
±
1.44%
1.06
0.32%,
respectively.
mainly
composed
fucose,
rhamnose,
arabinose,
galactose,
glucose,
galacturonic
glucuronic
acid,
with
molar
ratio
1.13:15.11:9.60:25.98:1.55:44.33:2.29,
may
be
acidic
heteropolysaccharide
containing
pyran
rings,
α-
β-glycosidic
bonds
acid.
Results
in
vitro
experiments
IC50
for
scavenging
0.759
mg/mL
1.758
mg/mL,
respectively;
values
inhibiting
0.634
2.178
concentration
25~200
μg/mL
no
obvious
toxicity
RAW264.7,
when
treated
100
CSPs,
relative
cell
phagocytosis
capacity
secreted
nitric
oxide
amount
153.75
12.01%
133.56
5.37%,
concentration-dependent
ability
inhibit
growth
within
test
0.25–2.0
mg/mL.
Summarizing
results,
due
excellent
antioxidant,
anti-tumor
expected
show
good
potential
comprehensive
development
food
medicine.
