Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 27, 2024
AbstractAbelmoschus
manihot
is
an
important
medicinal
and
edible
plant
known
for
its
functional
secondary
metabolites.
However,
little
about
the
key
genes
involved
in
production
of
metabolites
A.
manihot.
This
largely
due
to
a
lack
effective
gene
expression
detection
systems
manihot,
thus
screening
real-time
PCR
reference
prerequisite.
In
this
study,
11
candidate
were
screened
cloned
from
their
stability
was
evaluated
different
tissues
using
four
algorithms:
geNorm,
NormFinder,
BestKeeper,
RefFinder.
The
eIFPP2A1
highest,
while
that
tubulin
alpha
(TUA)
lowest.
combined
use
two
most
stable
genes,
PP2A1,
met
experimental
requirements
normalizing
Furthermore,
transcription
factors
bHLH147bHLH148
further
validated
by
data
normalization.
study
identified
potential
manihot
tissues,
paving
way
analysis
dissecting
metabolite
regulation
mechanisms
Ultrasonics Sonochemistry,
Journal Year:
2024,
Volume and Issue:
105, P. 106856 - 106856
Published: March 26, 2024
The
residue
remaining
after
oil
extraction
from
grape
seed
contain
abundant
procyanidins.
An
ultrasonic-assisted
enzyme
method
was
performed
to
achieve
a
high
efficiency
of
procyanidins
when
the
optimal
conditions
were
8
U/g
cellulase,
ultrasound
power
200
W,
ultrasonic
temperature
50
℃,
and
reaction
time
40
min.
effects
free
on
both
radical
scavenging
activity
thermal
stability
at
40,
60,
80
℃
procyanidins-loaded
liposomal
systems
prepared
by
discussed.
presence
concentrations
ranging
0.02
0.10
mg/mL
observed
be
effective
inhibiting
lipid
oxidation
15.15
%
69.70
in
linoleic
acid
model
system
during
for
168
h,
as
measured
using
ferric
thiocyanate
method.
characterized
measuring
mean
particle
size
encapsulation
efficiency.
Moreover,
holographic
plots
showed
that
effect-response
points
combined
with
α-tocopherol
liposomes
lower
than
addition
line
95
confidence
interval
limits.
At
same
time,
there
significant
differences
between
theoretical
IC
Molecules,
Journal Year:
2025,
Volume and Issue:
30(4), P. 830 - 830
Published: Feb. 11, 2025
This
study
optimized
the
extraction
process
of
crude
polysaccharides
from
Panax
notoginseng
leaves
(PNLP)
using
ultrasonic-assisted
dual-enzyme
method
through
a
single-factor
combined
with
response
surface
experiment.
The
were
subsequently
purified
and
isolated
DEAE-Cellulose
52,
followed
by
structural
analysis,
evaluation
antioxidant
activity,
examination
digestive
enzyme
inhibition.
hypoglycemic
effects
components
further
clarified.
results
indicated
that
polysaccharide
had
an
yield
17.13
±
0.29%.
fraction
PNLP-3
(eluted
0.3
M
NaCl)
was
obtained
52
chromatography,
exhibiting
total
sugar
content
81.2%
molecular
weight
16.57
kDa.
PNLP
is
primarily
composed
arabinose,
galactose,
galacturonic
acid,
molar
percentages
20.24%,
33.54%,
24.27%,
respectively.
mainly
arabinose
29.97%
49.35%,
In
this
IC50
values
for
α-glucosidase
α-amylase
inhibition
1.045
mg/mL
9.53
mg/mL,
Molecular
docking
confirmed
exhibits
better
inhibitory
activity
against
α-glucosidase.
Furthermore,
alleviated
hyperglycemia
in
insulin-resistant
HepG2
cells
enhancing
glucose
consumption
glycogen
synthesis.
exhibited
positive
correlation
its
concentration,
potentially
contributing
to
reducing
oxidative
stress.
These
findings
underscore
therapeutic
potential
leaf
managing
type
2
diabetes
offer
new
perspectives
on
use
natural
regulating
blood
glucose.
Plants,
Journal Year:
2025,
Volume and Issue:
14(5), P. 755 - 755
Published: March 1, 2025
This
review
highlights
the
recent
advancements
in
extraction
techniques
for
bioactive
compounds
from
natural
sources,
focusing
on
methodologies
that
enhance
both
efficiency
and
sustainability.
Techniques
such
as
pressurized
hot
water
(PHWE),
solid-state
fermentation
(SSF),
ionic
liquids
(ILs),
electrohydrodynamic
(EHD)
methods
have
shown
significant
potential
improving
yields
while
preserving
bioactivity
of
target
compounds.
These
innovative
approaches
offer
advantages
over
traditional
methods,
including
reduced
energy
consumption,
minimal
environmental
impact,
ability
to
extract
thermosensitive
PHWE
EHD
are
particularly
effective
extracting
antioxidants
compounds,
whereas
SSF
provides
an
environmentally
friendly
alternative
by
valorizing
agro-industrial
waste.
Ionic
liquids,
although
promising
complex
phytochemicals,
face
challenges
related
scalability
economic
feasibility.
The
adoption
these
advanced
represents
a
shift
toward
more
sustainable
cost-effective
processes,
promoting
discovery
utilization
high-value
also
contribute
development
eco-friendly,
strategies
align
with
green
chemistry
principles
regulatory
standards.
However,
further
research
technological
required
address
existing
limitations
ensure
widespread
application
industrial
pharmaceutical
sectors.
Ultrasonics Sonochemistry,
Journal Year:
2024,
Volume and Issue:
110, P. 107045 - 107045
Published: Aug. 24, 2024
In
this
study,
it
is
the
first
that
Viticis
Fructus
(VF)
was
used
as
raw
material
for
extracting
total
flavonoids
using
ultrasound-assisted
enzyme
extraction
(UAE)
method.
Response
surface
methodology
employed
to
determine
optimal
parameters.
The
conditions
were
follows:
60
%
ethanol
solution
extract
solvent,
material-liquid
ratio
of
1:25,
pH
value
4,
addition
amount
1.5
%,
enzymatic
hydrolysis
time
30
min,
temperature
40
℃,
and
ultrasonic
50
min.
Comparing
flavonoid
yield
VF
processed
(PVF)
extracted
different
methods,
observed
UAE
resulted
in
a
higher
compared
traditional
ultrasound
extraction.
Additionally,
PVF
by
all
three
methods
generally
than
VF.
also
demonstrated
better
vitro
antioxidant
activity
These
results
suggest
an
effective
method
enhance
natural
flavonoids.
study
physicochemical
properties
showed
significantly
increased
after
stir-frying,
indicating
their
efficacy
can
be
enhanced.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(2), P. 397 - 397
Published: Jan. 18, 2025
Acanthopanax
senticosus
(ASC)
contains
a
variety
of
bioactive
compounds
and
serves
as
an
important
traditional
Chinese
medicinal
resource.
However,
its
prolonged
growth
cycle
reliance
on
wild
populations
limit
practical
use.
To
explore
the
potential
sessiliflorus
(ASF)
alternative,
this
study
focused
optimizing
extraction
process
assessing
bioactivity
stem
extracts.
The
Analytic
Hierarchy
Process-Entropy
Weight
Method
(AHP-EWM)
was
applied
to
comprehensively
score
five
key
active
in
ASF
stems,
establishing
benchmark
for
evaluating
efficiency.
Ultrasound-assisted
enzymatic
(UAEE)
employed,
orthogonal
response
surface
experiments
were
conducted
refine
parameters.
optimal
conditions
identified
ultrasound
temperature
59
°C,
duration
57
min,
ethanol
concentration
61%,
liquid-to-material
ratio
39:1,
resulting
AHP-EWM
composite
94.76.
Comparative
analysis
antibacterial
antioxidant
activities
revealed
that
ASC
extracts
exhibited
superior
effects,
while
demonstrated
enhanced
properties.
These
findings
support
partial
replacement
with
ASF,
contributing
conservation
resources
expanding
application
development.
