Structural Characterization of Dendrobium officinale Polysaccharides and Their Regulation Effect on Intestinal Microbiota During In Vitro Fermentation DOI Open Access
Yanlei Yu, Honggang Wang, Jin Xiao

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(6), P. 727 - 727

Published: March 10, 2025

Polysaccharides derived from Dendrobium officinale have been demonstrated to exhibit metabolic regulatory properties. However, the correlation between their structure and function, particularly mechanism of action through gut microbiota, remains underexplored. This study systematically elucidates structural characteristics polysaccharide (DOP) Guizhou (GZ) Zhejiang (ZJ) provinces China using nuclear magnetic resonance (NMR) a series chromatographic analyses, revealing unique molecular features. Additionally, activities were assessed α-glucosidase inhibitory assay in vitro intestinal flora activity assay. The findings include following: (1) both DOP-GZ DOP-ZJ predominantly consist glycosidic linkages β-1,4-Manp β-1,4-Glcp; (2) zhe monosaccharide composition ratios mannose glucose are 2.51:1 for 2.66:1 DOP-ZJ, with weights 356 kDa 544 kDa, respectively, indicating significant differences DOPs different sources; (3) treatment led alterations α-diversity indices Firmicutes-to-Bacteroidota ratios; (4) more importantly, significantly increase abundance beneficial bacteria (e.g., g_Proteobacteria_unclassified) while suppressing growth pathogenic f_Enterobacteriaceae_unclassified), statistically results. These not only uncover novel by which regulate metabolism microbiota but also provide crucial theoretical basis application functional foods pharmaceutical development.

Language: Английский

Structural Characterization of Dendrobium officinale Polysaccharides and Their Regulation Effect on Intestinal Microbiota During In Vitro Fermentation DOI Open Access
Yanlei Yu, Honggang Wang, Jin Xiao

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(6), P. 727 - 727

Published: March 10, 2025

Polysaccharides derived from Dendrobium officinale have been demonstrated to exhibit metabolic regulatory properties. However, the correlation between their structure and function, particularly mechanism of action through gut microbiota, remains underexplored. This study systematically elucidates structural characteristics polysaccharide (DOP) Guizhou (GZ) Zhejiang (ZJ) provinces China using nuclear magnetic resonance (NMR) a series chromatographic analyses, revealing unique molecular features. Additionally, activities were assessed α-glucosidase inhibitory assay in vitro intestinal flora activity assay. The findings include following: (1) both DOP-GZ DOP-ZJ predominantly consist glycosidic linkages β-1,4-Manp β-1,4-Glcp; (2) zhe monosaccharide composition ratios mannose glucose are 2.51:1 for 2.66:1 DOP-ZJ, with weights 356 kDa 544 kDa, respectively, indicating significant differences DOPs different sources; (3) treatment led alterations α-diversity indices Firmicutes-to-Bacteroidota ratios; (4) more importantly, significantly increase abundance beneficial bacteria (e.g., g_Proteobacteria_unclassified) while suppressing growth pathogenic f_Enterobacteriaceae_unclassified), statistically results. These not only uncover novel by which regulate metabolism microbiota but also provide crucial theoretical basis application functional foods pharmaceutical development.

Language: Английский

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