Extracellular Vesicle MicroRNA in the Kidney DOI
Sekyung Oh, Chang Min Lee, Sang‐Ho Kwon

et al.

Comprehensive physiology, Journal Year: 2023, Volume and Issue: 13(3), P. 4833 - 4850

Published: June 26, 2023

Abstract Most cells in our body release membrane‐bound, nano‐sized particles into the extracellular milieu through cellular metabolic processes. Various types of macromolecules, reflecting physiological and pathological status producing cells, are packaged such so‐called vesicles (EVs), which can travel over a distance to target thereby transmitting donor cell information. The short, noncoding ribonucleic acid (RNA) called microRNA (miRNA) takes crucial part EV‐resident macromolecules. Notably, EVs transferring miRNAs induce alterations gene expression profiles recipient genetically instructed, base‐pairing interaction between their messenger RNAs (mRNAs), resulting either nucleolytic decay or translational halt engaged mRNAs. As other fluids, released urine, termed urinary (uEVs), carry specific sets miRNA molecules, indicate normal diseased states kidney, principal source uEVs. Studies have therefore been directed elucidate contents biological roles uEVs moreover utilize regulatory properties cargos ameliorating kidney diseases delivery via engineered EVs. We here review fundamental principles biology as well current understanding applications EV‐loaded kidney. further discuss limitations contemporary research approaches, suggesting future directions overcome difficulties advance both basic clinical treating diseases. © 2023 American Physiological Society. Compr Physiol 13:4833‐4850, 2023.

Language: Английский

Investigating the attenuating effects of metformin-loaded selenium nanoparticles coupled with Myrtus communis L. flower extract on CaOx deposition in male Sprague Dawley rat kidneys via regulating MAPK signaling pathway DOI Creative Commons
Jian Kang, YanQing Tong

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 14 - 26

Published: May 17, 2024

Kidney stones are a foremost clinical concern in urology with CaOx crystals accounting for roughly 80% of these renal formations. This research endeavor seeks to ascertain the protective effects Metformin-encapsulated selenium nanoparticles (M@Se NPs), combined 55% hydroethanolic flower extract from Myrtus communis L. (MCL) countering formation kidney Male Sprague Dawley rats. The particle's diameter was measured be 39 nm and 13.8 DLS HR-TEM analysis. Rat groups administered MCL-M@Se NPs (1:1.5:1) exhibited reduced stone urine serum analysis compared negative control group. Histological evaluations samples using H&E, MTS staining indicated subdued presence ECM deposition contrast other rat groups. Conclusively, mechanism against damage can confidently attributed obstruction MAPK signaling pathway.

Language: Английский

Citations

1
Expression profiles of urine exosomal tRNA-derived Small RNAs and their potential roles in calcium oxalate stone disease DOI Open Access
Senyuan Hong, Lin-Tao Miao,

Yuanyuan Yang

et al.

Annals of Medicine and Surgery, Journal Year: 2024, Volume and Issue: 86(10), P. 5802 - 5810

Published: Sept. 10, 2024

Language: Английский

Citations

1
Exosomes from miR-23 Overexpressing Stromal Cells Suppress M1 Macrophage and Inhibit Calcium Oxalate Deposition in Hyperoxaluria Rat Model DOI Creative Commons
Yifan Zhang,

Zhang Sheng-li,

Min Wang

et al.

BioMed Research International, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 10

Published: Nov. 16, 2023

Purpose. To investigate whether ADSC-derived miR-23-enriched exosomes could protect against calcium oxalate stone formation in a hyperoxaluria rat model. Methods. An ethylene glycol- (EG-) induced model and an vitro of COM-induced HK-2 cells coculturing with RAW264.7 were established to explore the protective mechanisms exosomes. Results. The results showed that treatment from ADSCs protected EG-induced rats, cell experiments confirmed alleviated autophagy. Overexpressed miR-23 suppressed M1 macrophage polarization by inhibiting IRF1 expression. Furthermore, predicted binding site between messenger RNA 3 ′ -untranslated region (3 id="M2"> -UTR) was dual-luciferase reporter assay. Conclusion. In conclusion, our research gave first evidence affected macrophages directly protecting

Language: Английский

Citations

3
Extracellular Vesicle MicroRNA in the Kidney DOI
Sekyung Oh, Chang Min Lee, Sang‐Ho Kwon

et al.

Comprehensive physiology, Journal Year: 2023, Volume and Issue: unknown, P. 4833 - 4850

Published: June 26, 2023

Most cells in our body release membrane-bound, nano-sized particles into the extracellular milieu through cellular metabolic processes. Various types of macromolecules, reflecting physiological and pathological status producing cells, are packaged such so-called vesicles (EVs), which can travel over a distance to target thereby transmitting donor cell information. The short, noncoding ribonucleic acid (RNA) called microRNA (miRNA) takes crucial part EV-resident macromolecules. Notably, EVs transferring miRNAs induce alterations gene expression profiles recipient genetically instructed, base-pairing interaction between their messenger RNAs (mRNAs), resulting either nucleolytic decay or translational halt engaged mRNAs. As other fluids, released urine, termed urinary (uEVs), carry specific sets miRNA molecules, indicate normal diseased states kidney, principal source uEVs. Studies have therefore been directed elucidate contents biological roles uEVs moreover utilize regulatory properties cargos ameliorating kidney diseases delivery via engineered EVs. We here review fundamental principles biology as well current understanding applications EV-loaded kidney. further discuss limitations contemporary research approaches, suggesting future directions overcome difficulties advance both basic clinical treating diseases. © 2023 American Physiological Society. Compr Physiol 13:4833-4850, 2023.

Language: Английский

Citations

2
Noncoding RNA, friend or foe for nephrolithiasis? DOI Creative Commons
Qing Wang, Zhenlu Yang, Xiaolong Chen

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 20, 2024

Nephrolithiasis is one of the most common diseases in urology, characterized by notable incidence and recurrence rates, leading to significant morbidity financial burden. Despite its prevalence, precise mechanisms underlying stone formation remain incompletely understood, thus hindering advancements kidney management over past three decades. Investigating pivotal biological molecules that govern has consistently been a challenging high-priority task. A portion mammalian genomes are transcribed into noncoding RNAs (ncRNAs), which have ability modulate gene expression disease progression. They emerging as novel target class for diagnostics pharmaceutical exploration. In recent years, role ncRNAs attracted burgeoning attention. found influence regulating ion transportation, oxidative stress injury, inflammation, osteoblastic transformation, autophagy, pyroptosis. These findings contributes new perspectives on pathogenesis nephrolithiasis. To enhance our understanding diagnostic therapeutic potential nephrolithiasis-associated ncRNAs, we summarized profiles, functions, clinical significance these current review.

Language: Английский

Citations

0
Extracellular Vesicle MicroRNA in the Kidney DOI
Sekyung Oh, Chang Min Lee, Sang‐Ho Kwon

et al.

Comprehensive physiology, Journal Year: 2023, Volume and Issue: 13(3), P. 4833 - 4850

Published: June 26, 2023

Abstract Most cells in our body release membrane‐bound, nano‐sized particles into the extracellular milieu through cellular metabolic processes. Various types of macromolecules, reflecting physiological and pathological status producing cells, are packaged such so‐called vesicles (EVs), which can travel over a distance to target thereby transmitting donor cell information. The short, noncoding ribonucleic acid (RNA) called microRNA (miRNA) takes crucial part EV‐resident macromolecules. Notably, EVs transferring miRNAs induce alterations gene expression profiles recipient genetically instructed, base‐pairing interaction between their messenger RNAs (mRNAs), resulting either nucleolytic decay or translational halt engaged mRNAs. As other fluids, released urine, termed urinary (uEVs), carry specific sets miRNA molecules, indicate normal diseased states kidney, principal source uEVs. Studies have therefore been directed elucidate contents biological roles uEVs moreover utilize regulatory properties cargos ameliorating kidney diseases delivery via engineered EVs. We here review fundamental principles biology as well current understanding applications EV‐loaded kidney. further discuss limitations contemporary research approaches, suggesting future directions overcome difficulties advance both basic clinical treating diseases. © 2023 American Physiological Society. Compr Physiol 13:4833‐4850, 2023.

Language: Английский

Citations

0