Virus-specific Dicer-substrate siRNA swarms inhibit SARS-CoV-2 infection in TMPRSS2-expressing Vero E6 cells DOI Creative Commons
Miao Jiang, Larissa Laine, Pekka Kolehmainen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 14, 2024

After 4 years of the COVID-19 pandemic, SARS-CoV-2 continues to circulate with epidemic waves caused by evolving new variants. Although rapid development vaccines and approved antiviral drugs has reduced virus transmission mitigated symptoms infection, continuous emergence variants lack simple-use (non-hospitalized, easy timing, local delivery, direct acting, host-targeting) treatment modalities have limited effectiveness drugs. Therefore, novel therapeutic approaches against infection are still urgently needed. As a positive-sense single-stranded RNA virus, is highly susceptible interference (RNAi). Accordingly, small interfering (si)RNAs targeting different regions genome can effectively block expression replication virus. However, genomic mutations led problem viral escape from targets RNAi strategy, which increased potential off-target effects siRNA decreased efficacy long-term use treatment. In our study, we enzymatically generated set Dicer-substrate (D)siRNA swarms containing DsiRNAs single or multiple conserved sequences using

Language: Английский

Enhanced Humoral and Cellular Immune Responses Elicited by Salmonella Flagellin-Adjuvanted SARS-CoV-2 S1 Subunit Vaccine DOI
Song Li,

Yaodan Cui,

Qiaoju Wang

et al.

Viral Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, has been spreading and changing globally. Adjuvant-based vaccines can improve vaccine protection by enhancing immune response. Bacterial flagellin is a potent adjuvant promotes protective responses. Here, we successfully expressed purified S1 subunit of SARS-CoV-2. The adjuvanticity (FliC) Salmonella Typhimurium in mice was determined combining it with recombinant vaccine. FliC-adjuvanted could induce significantly enhanced S1-specific Immunoglobulin G (IgG), IgG1 IgG2a titers, SARS-CoV-2-neutralizing antibodies, levels Th1 type (TNF-α IFN-γ) Th2 (Interleukin-5 (IL-5), IL-4, IL-10, IL-13) cytokines splenocytes compared alone group. Additionally, titers IgG antibodies FliC group maintain high level for at least months. These results indicated trigger strong humoral cellular responses, which promote ongoing development COVID-19 vaccines.

Language: Английский

Citations

0
Escherichia coli Nissle 1917 efficiently expresses the RBD domain of SARS-CoV-2 spike protein without codon optimization DOI Creative Commons
Shanmugaraja Meenakshi,

R Suvetha,

S. Ramadevi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 5, 2025

Bacterial outer membrane vesicles (OMVs) represent a promising and versatile platform for vaccine delivery. Their inherent self-adjuvant properties the ability to be adorned with wide range of heterogeneous antigens position them as powerful tool in fight against infectious diseases. Escherichia coli Nissle 1917 (EcN) stands out highly valuable probiotic strain because its long history safe use proven clinical benefits humans. The EcN was genetically engineered derive OMVs displaying receptor binding domain (RBD) SARS-CoV-2 spike protein on their surface. Although some research groups have previously expressed viral or RBD E. coli, particularly EcN, this study shows maiden effort utilize gene encoding native RBD. exhibited significant level expression, demonstrating more efficient codon usage pattern compared commonly used bacterial expression systems such BL21, DH5α. display form surface using Lpp-OmpA system. Cell fractionation studies clearly indicated presence fraction. were isolated ultracentrifugation confirmed by western blot followed immunofluorescence analyses. Due preferential uptake antigen presenting cells, derived from bearing hold promise potential COVID-19 candidate.

Language: Английский

Citations

0
Prevalence of the Novel Recombinant LSDV in East and Southeast Asia: Inducing Skin and Testicular Damage in Golden Hamsters DOI
Jingyu Wang,

Shaobing Wan,

Shizhe Liu

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 197, P. 107057 - 107057

Published: Oct. 22, 2024

Language: Английский

Citations

3
Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines DOI Creative Commons

Maofeng Wang,

Cancan Wu,

Nan Liu

et al.

Engineering Microbiology, Journal Year: 2024, Volume and Issue: 4(3), P. 100162 - 100162

Published: June 26, 2024

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the importance of developing novel vaccines. An ideal vaccine should trigger an intense immune reaction without causing significant side effects. In this study we found that substitution tryptophan located in cores severe acute respiratory syndrome 2 (SARS-CoV-2) protein structures with certain smaller amino acids resulted variants melting temperatures 33–37 °C. enzyme activity assay indicated proteolytic main proteinase (3CLpro) decreased sharply when environmental temperature exceeded temperature, implying other may lose most their functions under same conditions. This finding suggests a virus variant containing engineered proteins °C only be functional upper tract where is about 33 °C, but will unable to invade internal organs, which maintain above 37 thus making it possible construct temperature-sensitive attenuated

Language: Английский

Citations

2
Quinoin, type 1 ribosome inactivating protein alters SARS-CoV-2 viral replication organelle restricting viral replication and spread DOI Creative Commons

Sofia Maria Luigia Tiano,

Nicola Landi,

Valentina Marano

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135700 - 135700

Published: Sept. 1, 2024

Language: Английский

Citations

1
Coronavirus spike protein-based vaccines. Vaccine delivery systems DOI Creative Commons
Akmal M. Asrorov, Mirzakamol S. Ayubov, Bin Tu

et al.

Medicine in Drug Discovery, Journal Year: 2024, Volume and Issue: unknown, P. 100198 - 100198

Published: Sept. 1, 2024

Language: Английский

Citations

0
Prokaryote- and Eukaryote-Based Expression Systems: Advances in Post-Pandemic Viral Antigen Production for Vaccines DOI Open Access

Nelli S. Khudainazarova,

Dmitriy L. Granovskiy,

О. А. Кондакова

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11979 - 11979

Published: Nov. 7, 2024

This review addresses the ongoing global challenge posed by emerging and evolving viral diseases, underscoring need for innovative vaccine development strategies. It focuses on modern approaches to creating vaccines based recombinant proteins produced in different expression systems, including bacteria, yeast, plants, insects, mammals. analyses advantages, limitations, applications of these systems producing antigens, as well strategies designing safer, more effective, potentially 'universal' antigens. The discusses a range excluding SARS-CoV-2, which has already been extensively studied. authors present findings with aim contributing research advancing antiviral vaccines.

Language: Английский

Citations

0
Virus-specific Dicer-substrate siRNA swarms inhibit SARS-CoV-2 infection in TMPRSS2-expressing Vero E6 cells DOI Creative Commons
Miao Jiang, Larissa Laine, Pekka Kolehmainen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 14, 2024

After 4 years of the COVID-19 pandemic, SARS-CoV-2 continues to circulate with epidemic waves caused by evolving new variants. Although rapid development vaccines and approved antiviral drugs has reduced virus transmission mitigated symptoms infection, continuous emergence variants lack simple-use (non-hospitalized, easy timing, local delivery, direct acting, host-targeting) treatment modalities have limited effectiveness drugs. Therefore, novel therapeutic approaches against infection are still urgently needed. As a positive-sense single-stranded RNA virus, is highly susceptible interference (RNAi). Accordingly, small interfering (si)RNAs targeting different regions genome can effectively block expression replication virus. However, genomic mutations led problem viral escape from targets RNAi strategy, which increased potential off-target effects siRNA decreased efficacy long-term use treatment. In our study, we enzymatically generated set Dicer-substrate (D)siRNA swarms containing DsiRNAs single or multiple conserved sequences using

Language: Английский

Citations

0