Pseudotyped Viruses: A Useful Platform for Pre-Clinical Studies Conducted in a BSL-2 Laboratory Setting

Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 135 - 135

Published: Jan. 15, 2025

The study of pathogenic viruses has always posed significant biosafety challenges. In particular, the highly requires methods with low biological risk but relatively high sensitivity and convenience in detection. recent years, pseudoviruses, which consist a backbone one virus envelope proteins another virus, have become most widely used tools for exploring mechanisms binding to cells, membrane fusion viral entry, as well screening libraries antiviral substances, evaluating potential neutralizing monoclonal antibodies, developing neutralization tests, therapeutic platforms. During outbreak severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), pseudotyped virus-based assays played pivotal role advancing our understanding virus-cell interactions its disease pathogenesis. Such facilitated search agents accelerated epidemiological studies on post-infection post-vaccination humoral immunity. This review focuses use pseudoviruses model large-scale applications enveloped viruses.

Language: Английский

Coronavirus spike protein-based vaccines. Vaccine delivery systems

Medicine in Drug Discovery, Journal Year: 2024, Volume and Issue: unknown, P. 100198 - 100198

Published: Sept. 1, 2024

Language: Английский