Genomic characterization of Listeria monocytogenes and Listeria innocua isolated from milk and dairy samples in Ethiopia

BMC Genomic Data, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 31, 2024

Abstract Listeriosis caused by Listeria monocytogenes often poses a significant threat to vulnerable populations. Dairy products have been implicated in outbreaks of listeriosis worldwide. In Ethiopia, studies identified spp. and L. various dairy products, but the genetic diversity phylogenetic relationships these bacteria remain largely unknown low- middle-income countries. Therefore, we conducted whole-genome sequencing on 15 55 innocua isolates obtained from different levels supply chains across three regions Ethiopia. Genomes were assembled used for MLST genotyping single nucleotide polymorphism (SNP) analysis infer relationships. We total 3 (i.e., 2, 145, 18) 12 1489, 1619, 603, 537, 1010, 3186, 492, 3007, 1087, 474, 1008, 637) sequence types among studied isolates. Some showed region-specific occurrence, while others broadly distributed regions. Through high-quality SNP analysis, found that 13 as ST 11 them highly similar with low variation, differing only 1 10 SNPs, suggesting potential selection food chain. The also exhibited intra-ST variation 0–10 differences, except which displayed greater diversity.

Language: Английский

---

Phage-Based Control of Listeria innocua in the Food Industry: A Strategy for Preventing Listeria monocytogenes Persistence in Biofilms

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 482 - 482

Published: March 27, 2025

Listeria innocua, though considered non-pathogenic, frequently coexists with monocytogenes in industrial environments, aiding its survival biofilms. These biofilms pose a significant challenge food processing facilities, as they protect bacteria from disinfectants and facilitate their spread. The aim of this review was to identify bacteriophages promising method for eliminating the industry. Lytic show great potential combating Commercially available products, such PhageGuard Listex™ (P100) (Micreos Food Safety, Wageningen, Netherlands), effectively reduce both L. innocua products on production surfaces. Additionally, phage-derived enzymes, endolysins, can degrade biofilms, without compromising quality. following article highlights that although present biocontrol method, further research is necessary assess long-term effectiveness, particularly regarding bacterial resistance. To maximize efficacy, combination strategies phage cocktails recommended enhance biofilm eradication minimize contamination risks.

Language: Английский

---

Genomic Characterization of Listeria innocua Isolates Recovered from Cattle Farms, Beef Abattoirs, and Retail Outlets in Gauteng Province, South Africa

Pathogens, Journal Year: 2023, Volume and Issue: 12(8), P. 1062 - 1062

Published: Aug. 18, 2023

Whole-genome sequencing (WGS) was used for the genomic characterization of one hundred and ten strains Listeria innocua (L. innocua) isolated from twenty-three cattle farms, eight beef abattoirs, forty-eight retail outlets in Gauteng province, South Africa. In silico multilocus sequence typing (MLST) to identify isolates’ types (STs). BLAST-based analyses were antimicrobial virulence genes. The study also linked detection genes origin (industries samples) L. isolates. detected 14 STs, 13 resistance genes, 23 Of STs detected, ST637 (26.4%), ST448 (20%), 537 (13.6%), 1085 (12.7%) predominant, frequency varied significantly (p < 0.05). All 110 isolates carriers or more with lin (100%), fosX tet(M) (30%) being most frequently recognized, (clpC, clpE, clpP, hbp1, svpA, hbp2, iap/cwhA, lap, lpeA, lplA1, lspA, oatA, pdgA, prsA2) found all innocua. Overall, diversity significant differences frequencies resistance, according origins (source sample type) This, first recovered three levels/industries (farm, abattoir, retail) production system Africa, provides data on organism’s distribution potential food safety implications.

Language: Английский

---

A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

Tropical Animal Health and Production, Journal Year: 2024, Volume and Issue: 56(2)

Published: Feb. 27, 2024

Abstract These cross-sectional studies reported the occurrence, genetic characteristics, and factors associated with distribution of Listeria species on cattle farms beef abattoirs in Gauteng Province, South Africa. A total 328 samples (faeces, feeds, silage, drinking water) were collected from 23 (communal, cow-calf, feedlot), 262 carcass swabs, effluents) 8 (low throughput high throughput) processed using standard bacteriological molecular methods to detect species. The prevalence investigated, multiplex polymerase chain reaction (mPCR) was used determine species, pathogenic serogroups, carriage eight virulence-associated genes by monocytogenes . overall 14.6%, comprising innocua (11.3%), (3.4%), welshimeri (0.0%) compared 11.1%, (5.7%), (4.6%), (0.8%) for abattoirs. Of three variables (area, type farm/abattoir, sample type) only types at had a significant ( P < 0.001) effect L. welshimeri. frequency serogroups based 11 isolated 72.7% 27.3% serogroup 1/2a-3a 4b-4d-4e, respectively, while 12 isolates recovered abattoirs, it 25%, 8.3%, 50% 16.7% 1/2a-3a, 1/2b-3b, 1/2c-3c, 4b-4d-4e respectively (P 0.05). All (100%) positive seven virulence hlyA , inlB plcA iap inlA inlC, inlJ) clinical food safety significance findings cannot be ignored.

Language: Английский

---

Listeriosis: Characteristics, Occurrence in Domestic Animals, Public Health Significance, Surveillance and Control

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 2055 - 2055

Published: Oct. 12, 2024

Listeriosis is a dangerous zoonosis caused by bacteria of the genus

Language: Английский

---

Genomic Characterization of <em>Listeria innocua</em> Isolates Recovered from Cattle Farms, Beef Abattoirs, and Retail Outlets in Gauteng Province, South Africa

Published: July 10, 2023

Whole-genome sequencing (WGS) was used for genomic characterization of 110 strains Listeria innocua isolated from 23 cattle farms, 8 beef abattoirs, and 48 retail outlets in Gauteng province, South Africa. In silico multilocus sequence typing (MLST) to identify the isolates&#039; types (STs). BLAST-based analyses were antimicrobial virulence genes. The study also linked detection genes origin (sources samples) L. isolates. detected 12 STs, 13 resistance genes, Of STs de-tected, ST637 (26.4%), ST448 (20%), 537 (13.6%), 1085 (12.7%) predominant, frequency varied significantly (P&lt;0.05). All isolates car-riers one or more with lin (100%), fosX tet(M) (30%) being most frequently recognized, (clpC, clpE, clpP, hbp1.svpA, hbp2, iap/cwhA, lap, lpeA, lplA1, lspA, oatA, pdgA, prsA2) found all innocua. Overall, diversity significant differences frequencies resistance, according origins (source sample type) This, first recovered three levels (farm, abattoir, outlets) production system Africa, provides data on organism&#039;s distribution potential food safety implications..

Language: Английский

---

Whole genome sequencing reveals circulation of potentially virulent Listeria innocua strains with novel genomic features in cattle farm environments in Dhaka, Bangladesh

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: unknown, P. 105692 - 105692

Published: Nov. 1, 2024

Language: Английский

---

Prevalence, virulence potential, antibiotic resistance profile, heavy metal resistance genes of Listeria innocua: A first study in consumed foods for assessment of human health risk in Northern Turkey

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Language: Английский

---

ҚҰНАРЛАНДЫРЫЛҒАН АЗЫҚТАРДА ЛИСТЕРИЯЛАРДЫҢ ӨМІРШЕҢДІГІН САЛЫСТЫРМАЛЫ БАҒАЛАУ

Ġylym ža̋ne bìlìm, Journal Year: 2023, Volume and Issue: 1(4(73)), P. 11 - 22

Published: Dec. 25, 2023

Стерильді сынамалардағы тәжірибедегі зерттеу нәтижелері листерияның жемшөп массасында көбейіп, жиналып, максималды концентрацияға жете алатындығын көрсетті. Сонымен, бастапқы рН 7,08-7,19 кезінде барлық сынамаларда листерия өсуге жетті. Тоңазытқыш жағдайында 40 С концентрациясы 3-5 тәулікте 111,9-606,6 мың КТБ/г құрады, ұқсас 180С бөлме температурасы 2-4 5166,6-11783,3 КҚ/г Термостатта 370С мұндай айтарлықтай дейін өсті және 13945,0-22216,0 деңгейінде болды. емес үлгілермен жүргізілген тәжірибелер көрсеткендей, тоңазытқыш 40С сынамалардын рН-да аздап ауытқып, тәжірибе барысында деңгейде сақталды 11 7,11-7,18 ал бұл температуралық режимдегі концентрация өзгерді, бірақ 9-11 артып 18,1-82,5 болды. Бөлме температурасында артып, 5-7 тәулікке 18,6-152,2 жетті, сынамалардың рН-тын төмендеуіне байланысты 0,35-53,3 қысқарылды. Термостат режимде 2 өсті. Осылайша, нәтижелер құрама жемдегі листериялардың өміршеңдігі температура режиміне, пен ілеспе микрофлораға екенін көрсетеді.

Language: Русский

---