An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals DOI Creative Commons
Anna Offersgaard,

Carlos R. Duarte Hernandez,

Yuyong Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 24, 2024

Rapidly waning immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires continued global access to affordable vaccines. Globally, inactivated SARS-CoV-2 vaccines have been widely used during the pandemic. In this proof-of-concept study we adapted an original-D614G virus Vero cell culture as a strategy enhance vaccine manufacturing productivity. A passage 60 (P60) showed enhanced fitness and 50-fold increased yield in bioreactor compared virus. It further remained susceptible neutralization by plasma from vaccinated convalescent individuals, suggesting exposure of relevant epitopes. Monovalent P60 bivalent P60/omicron BA.1 induced neutralizing responses viruses mice hamsters, demonstrating that is suitable antigen. Antibodies cross-neutralized delta BA.5 viruses. Importantly, protected hamsters disease upon challenge with or virus, minimal lung pathology lower loads upper airways. Antigenicity was thus retained original despite acquisition adaptive mutations. Consequently, adaptation may be useful approach increase yields antigen production.

Language: Английский

An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals DOI Creative Commons
Anna Offersgaard,

Carlos R. Duarte Hernandez,

Yuyong Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 24, 2024

Rapidly waning immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires continued global access to affordable vaccines. Globally, inactivated SARS-CoV-2 vaccines have been widely used during the pandemic. In this proof-of-concept study we adapted an original-D614G virus Vero cell culture as a strategy enhance vaccine manufacturing productivity. A passage 60 (P60) showed enhanced fitness and 50-fold increased yield in bioreactor compared virus. It further remained susceptible neutralization by plasma from vaccinated convalescent individuals, suggesting exposure of relevant epitopes. Monovalent P60 bivalent P60/omicron BA.1 induced neutralizing responses viruses mice hamsters, demonstrating that is suitable antigen. Antibodies cross-neutralized delta BA.5 viruses. Importantly, protected hamsters disease upon challenge with or virus, minimal lung pathology lower loads upper airways. Antigenicity was thus retained original despite acquisition adaptive mutations. Consequently, adaptation may be useful approach increase yields antigen production.

Language: Английский

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