Unveiling unique microbial nitrogen cycling and nitrification driver in coastal Antarctica

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 12, 2024

Abstract Largely removed from anthropogenic delivery of nitrogen (N), Antarctica has notably low levels nitrogen. Though our understanding biological sources ammonia have been elucidated, the microbial drivers nitrate (NO 3 − ) cycling in coastal remains poorly understood. Here, we explore N Antarctica, unraveling origin NO via oxygen isotopes soil and lake sediment, through reconstruction 1968 metagenome-assembled genomes 29 phyla. Our analysis reveals metabolic potential for 2 fixation, nitrification, denitrification, but not anaerobic ammonium oxidation, signifying a unique N-cycling dynamic. We identify predominance complete oxidizing (comammox) Nitrospira , capable performing entire nitrification process. Their adaptive strategies to Antarctic environment likely include synthesis trehalose cold stress, high substrate affinity resource utilization, alternate pathways nutrient-scarce conditions. confirm significant role comammox autotrophic, process 13 C-DNA-based stable isotope probing. This research highlights crucial contribution budget identifying clade B as driver.

Language: Английский

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Insights into Nitrous Oxide Mitigation Strategies in Wastewater Treatment and Challenges for Wider Implementation

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(11), P. 7208 - 7224

Published: May 12, 2021

Nitrous oxide (N2O) emissions account for the majority of carbon footprint wastewater treatment plants (WWTPs). Many N2O mitigation strategies have since been developed while a holistic view is still missing. This article reviews state-of-the-art studies in treatment. Through analyzing existing studies, this presents essential knowledge to guide mitigations, and logics behind strategies. In practice, mitigations are mainly carried out by aeration control, feed scheme optimization, process optimization. Despite increasingly more real implementation remains rare, which combined result unclear climate change policies/incentives, as well technical challenges. Five critical challenges, opportunities, were identified. It proposed that (i) quantification methods overall pathway contributions need improvement; (ii) reliable straightforward mathematical model required quantify benefits compare strategies; (iii) tailored risk assessment needs be conducted WWTPs, long-term full-scale trials urgently needed enable robust assessments resulting operational costs impact on nutrient removal performance; (iv) current focus centralized investigations warranted decentralised systems, especially decentralized activated sludge WWTPs; (v) may mitigated adopting novel promoting reduction denitrification or microorganisms emit less N2O. Overall, we conclude research reaching maturity challenges exist wider implementation, relation reliability potential risks performances WWTPs.

Language: Английский

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Niche specialization of comammox Nitrospira in terrestrial ecosystems: Oligotrophic or copiotrophic?

Critical Reviews in Environmental Science and Technology, Journal Year: 2022, Volume and Issue: 53(2), P. 161 - 176

Published: March 20, 2022

The discovery of complete ammonia oxidizers, comammox Nitrospira, represents a breakthrough in the history nitrification research. Nitrospira inopinata, which was obtained from an aquatic ecosystem, is only pure bacterial isolate reported so far. Ammonia oxidation kinetics N. inopinata indicated that they prefer oligotrophic lifestyle and may directly compete with ammonia-oxidizing archaea (AOA), but representatives terrestrial ecosystems are lacking. Current studies demonstrate functionally dominant separated most sequences engineering systems. Clades A B seemingly have different ecological preferences soils, possibly due to their ammonium uptake Analyses evolutionary indicate some genes involved could be laterally transferred β-ammonia-oxidizing bacteria (AOB). Some species similar AOB active niches where previously considered dominated by AOB. Taken together, this review summarizes recent findings biogeographical distribution preference large-scale soil surveys, responses nitrogen application soil, putative mechanisms underpinning niche Nitrospira. These suggest not strictly both copiotrophic broader breadth. Comammox abundant environments. profoundly expanded our knowledge environmental relative contribution soils.

Language: Английский

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Microbial Biogeochemical Cycling of Nitrogen in Arid Ecosystems

Microbiology and Molecular Biology Reviews, Journal Year: 2022, Volume and Issue: 86(2)

Published: April 7, 2022

Arid ecosystems cover ∼40% of the Earth's terrestrial surface and store a high proportion global nitrogen (N) pool. They are low-productivity, low-biomass, polyextreme ecosystems, i.e., with (hyper)arid (hyper)oligotrophic conditions UV irradiation evapotranspiration. These severely limit presence macrofauna -flora and, particularly, growth productivity plant species. Therefore, it is generally recognized that much primary production (including N-input processes) nutrient biogeochemical cycling (particularly N cycling) in these microbially mediated. Consequently, we present comprehensive survey current state knowledge biotic abiotic N-cycling processes edaphic (i.e., open soil, biological soil crust, or plant-associated rhizosphere rhizosheath) hypo/endolithic refuge niches from drylands general, including hot, cold, polar desert ecosystems. We particularly focused on mediated fixation, mineralization, assimilatory dissimilatory nitrate reduction, nitrification denitrification anaerobic ammonium oxidation (anammox) N-loss processes. note application modern meta-omics related methods has generated data sets abundance, diversity, ecology different microbial guilds. However, worth mentioning important deserts (e.g., Sahara) quantitative rate transformation various lacking sparse. Filling this gap important, as climate change models often lack activity environmental communities can be key actors by producing consuming nitrous oxide (N2O), potent greenhouse gas.

Language: Английский

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Effects of acidification on nitrification and associated nitrous oxide emission in estuarine and coastal waters

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 13, 2023

In the context of an increasing atmospheric carbon dioxide (CO2) level, acidification estuarine and coastal waters is greatly exacerbated by land-derived nutrient inputs, upwelling, complex biogeochemical processes. A deeper understanding how nitrifiers respond to intensifying thus crucial predict response ecosystems their contribution global climate change. Here, we show that can significantly decrease nitrification rate but stimulate generation byproduct nitrous oxide (N2O) in waters. By varying CO2 concentration pH independently, expected beneficial effect elevated on activity ("CO2-fertilization" effect) excluded under acidification. Metatranscriptome data further demonstrate could up-regulate gene expressions associated with intracellular homeostasis cope stress. This study highlights molecular underpinnings effects greenhouse gas N2O emission, helps evolution change human activities.

Language: Английский

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Chlorate as a comammox Nitrospira specific inhibitor reveals nitrification and N2O production activity in coastal wetland

Soil Biology and Biochemistry, Journal Year: 2022, Volume and Issue: 173, P. 108782 - 108782

Published: July 30, 2022

Language: Английский

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Full substitution of chemical fertilizer by organic manure decreases soil N₂O emissions driven by ammonia oxidizers and gross nitrogen transformations

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(24), P. 7117 - 7130

Published: Oct. 6, 2023

Replacing synthetic fertilizer by organic manure has been shown to reduce emissions of nitrous oxide (N2 O), but the specific roles ammonia oxidizing microorganisms and gross nitrogen (N) transformation in regulating N2 O remain unclear. Here, we examined effect completely replacing chemical with on emissions, oxidizers, N rates using a 13-year field manipulation experiment. Our results showed that reduced cumulative 16.3%-210.3% compared fertilizer. The abundance bacteria (AOB) was significantly lower during three growth stages maize. Organic also decreased AOB alpha diversity changed their community structure. However, substitution increased archaea comammox Nitrospira Interestingly, mineralization 23.2%-32.9%, autotrophic nitrification rate 10.5%-45.4%, when This study found positive correlation between abundance, emission, contribution emission supported random forest analysis. highlights key oxidizers predicting cropland O.

Language: Английский

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Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N₂O production

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(7), P. 1984 - 1997

Published: Jan. 6, 2023

The contribution of agriculture to the sustainable development goals requires climate-smart and profitable farm innovations. Increasing ammonia fertilizer applications meet global food demands results in high agricultural costs, environmental quality deterioration, warming, without a significant increase crop yield. Here, we reported that third microbial oxidation process, complete (comammox), is contributing loss (41.9 ± 4.8%) at rate 3.53 0.55 mg N kg-1 day-1 soils around world. comammox loss, occurring mainly surface soil profiles (0-0.2 m), was equivalent bacterial (48.6 4.5%); both processes were significantly more important than archaeal (9.5 3.6%). In contrast, produced less N2 O (0.98 0.44 μg , 11.7 3.1%), comparable by (16.4 4.4%) but lower (72.0 5.1%). efficiency conversion (0.02 0.01%) evidently (0.24 0.06%) (0.16 0.04%) oxidation. increased with increasing pH values, which only physicochemical characteristic influenced abundance rates. Ammonia dominated oxidation, intense >6.5 <6.5. Our revealed plays vital role agroecosystems have been previously overlooked for long term.

Language: Английский

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Sustainable wastewater management through nitrogen-cycling microorganisms

Nature Water, Journal Year: 2024, Volume and Issue: 2(10), P. 936 - 952

Published: Oct. 14, 2024

Language: Английский

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A critical review of comammox and synergistic nitrogen removal coupling anammox: Mechanisms and regulatory strategies

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 948, P. 174855 - 174855

Published: July 20, 2024

Language: Английский

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