Seasonal dynamics of bacterial community structure and function in the surf zone seawater of a recreational beach in Ostend, Belgium DOI Creative Commons
Yunmeng Li, Pascal I. Hablützel, Zixia Liu

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(6)

Published: Nov. 8, 2024

Despite the importance of bacteria in surf zone water quality, detailed insights into their community composition, functions, and seasonal dynamics at recreational beaches are scarce. This study conducted year-long, weekly monitoring bacterial communities environmental factors a beach Ostend, Belgium. Using full-length 16S rRNA gene sequencing, we correlated composition predicted functions with to identify potential drivers. Bacterial were significantly affected by variations chlorophyll (Chl a), net primary productivity (NPP), seawater temperature (SWT), minimal influence from faecal inputs due human activities. Spring showed distinct abundances Planktomarina, Amylibacter, Sulfitobacter, positively Chl related sulphur oxidation potential. Summer had higher Cryomorphaceae, likely enhancing chemoheterotrophy. Beginning mid late fall extending winter, underwent substantial changes. Fall featured distinctive enrichment Thioglobaceae, inversely a. Winter was dominated Methylophilaceae (OM43 clade), negatively a, NPP, SWT. Both seasons exhibited elevated levels potentially pathogenic phenotypes methanol methylotrophy. provides baseline for understanding how respond changes impact health.

Language: Английский

Community coalescence under variable hydrochemical conditions of the Chesapeake Bay shaped bacterial diversity and functional traits DOI
Zhenyu Huang, Longfei Shu, Zhili He

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 257, P. 119272 - 119272

Published: May 31, 2024

Language: Английский

Citations

5
Bacterial Community Analysis of Recreational Beach Waters Reveals Human Fecal Contamination and Pathogenicity across Varying Field Conditions DOI Creative Commons
Dong Li,

Laurie C. Van De Werfhorst,

Rachel T. Noble

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123697 - 123697

Published: April 1, 2025

Assessing human fecal contamination of recreational beach waters is a public health challenge owing to multiple sources and environmental conditions affecting indicator, pathogen, source marker relationships. Human fecal-associated markers, such as HF183, accurately indicate waste, but reliance on individual markers risks false negative diagnoses, loading variations attenuation. Bacterial community analysis data-dense approach that could improve the accuracy waste detection, relatedness actual hazards under complex coastal water unproven. Here, two Malibu one Pacific Palisades, CA beaches differing in urbanization sewerage were studied over years across varying seasonal rainfall, berm, conditions. Fecal indicator bacteria (FIB), norovirus concentrations quantified related bacterial 16S rRNA gene sequences which further analyzed for putative pathogens sewage proportions. All lower watersheds harbored sources, surf zone was enhanced by rainfall runoff with berm breaching, intensified urbanization. Notably, sequenced sampled all weather (n = 36), sequence-based pathogen proportions correlated qPCR HF183 (w/ w/o PMA treatment; p 0.0) G1concentrations (p 0.04) also sequence abundance communities 0.02). Although appeared vary seasonally range physical influenced contamination, associated hazard reliably evidenced this study.

Language: Английский

Citations

0
SourceApp: A Novel Metagenomic Source Tracking Tool that can Distinguish between Fecal Microbiomes Using Genome-To-Source Associations Benchmarked Against Mixed Input Spike-In Mesocosms DOI Creative Commons
Blake G. Lindner, Katherine E. Graham,

Jacob R. Phaneuf

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Methodologies utilizing metagenomics are attractive to fecal source tracking (FST) aims for assessing the presence and proportions of various inputs simultaneously. Yet, compared established culture- or PCR-based techniques, metagenomic approaches these purposes rarely benchmarked contextualized practice. We performed shotgun sequencing experiments (n = 35) mesocosms constructed from water a well-studied recreational drinking reservoir spiked with 6 animal sources, 3 wastewater 1 septage source) synthetic microbiome spike-ins 1) introduced at predetermined cell concentrations simulate pollution events known composition. built source-associated genome databases using publicly available reference genomes metagenome assembled (MAGs) recovered short- long-read spike-ins, then created an associated bioinformatic tool, called SourceApp, inferring attribution apportionment by mapping data databases. SourceApp's performance varied substantially source, cows being underestimated due under sampling cow microbiomes. Parameter tuning revealed sensitivity specificity near 0.90 overall, which exceeded all alternative tools. SourceApp can assist researchers analyzing interpreting developing standard operating procedures on frontiers FST.

Language: Английский

Citations

0
Probabilistic fecal pollution source profiling and microbial source tracking for an urban river catchment DOI Creative Commons
Julia Derx,

H. Seda Kılıç,

Rita Linke

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 857, P. 159533 - 159533

Published: Oct. 18, 2022

We developed an innovative approach to estimate the occurrence and extent of fecal pollution sources for urban river catchments. The methodology consists 1) catchment surveys complemented by literature data where needed probabilistic estimates daily produced indicator (FIBs, E. coli, enterococci) zoonotic reference pathogen numbers (Campylobacter, Cryptosporidium Giardia) excreted human animal in a catchment, 2) generating hypothesis about dominant selecting source targeted monitoring design, 3) verifying results comparing measured concentrations informed choice parameters (i.e. chemical tracers, C. perfringensspores, host-associated genetic microbial tracking (MST) markers) river, multi-parametric correlation analysis. tested at study area Vienna, Austria. particle according indicated that, dry weather scenario, discharge treated wastewater (WWTP) was primary contributor pollution. For wet 80–99 % FIBs pathogens resulted from combined sewer overflows (CSOs) estimates. When testing our marker were log10 4 higher than selected markers. Our analyses showed first-time statistical relationships between perfringens spores (used as conservative tracer communal sewage) HF183/BacR287) with Giardia water (Spearman rank correlation: 0.78–0.83, p < 0.05. facilitates safety management provides robust basis fate transport models infection risk assessment.

Language: Английский

Citations

15
Novel Microbial Engraftment Trajectories Following Microbiota Transplant Therapy in Ulcerative Colitis DOI
Daphne Moutsoglou,

Aneesh Syal,

Sharon Lopez

et al.

Journal of Crohn s and Colitis, Journal Year: 2024, Volume and Issue: 19(2)

Published: Sept. 6, 2024

Abstract Background and Aims Microbiota transplant therapy (MTT) is an emerging treatment for ulcerative colitis (UC). One proposed mechanism the benefit of MTT through engraftment donor microbiota; however, kinetics are unknown. We identified SourceTracker as efficient method both to determine kinetic study engrafting taxa aid in determining how this may treat UC. Methods Ulcerative patients received either encapsulated (drug name MTP-101C) or placebo capsules daily 8 weeks followed by a 4-week washout period. Amplicon sequence data from donors were analyzed using Bayesian algorithm SourceTracker. Results Twenty-seven enrolled, 14 13 MTT. Baseline Shannon Chao1 indices negatively correlated with week 12 treated active drug but not patients. positively distance measured Bray–Curtis similarity metric placebo. Engraftment at was significantly higher group than group. our quantified proportion during 1 (active treatment) 12, 4 after last dose. Conclusion can be used simple reliable quantify microbial community contribution UC other inflammatory conditions

Language: Английский

Citations

2
Exploring the Bacterial Community in Aged Fecal Sources from Dairy Cows: Impacts on Fecal Source Tracking DOI Creative Commons
Megan Devane, William Taylor, Pierre‐Yves Dupont

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1161 - 1161

Published: April 28, 2023

(1) Background: This paper discusses the impact of agricultural activities on stream health, particularly in relation to dairy cow fecal pollution. The study explores microbiome cattle and potential ecological implications aging pollution waterways. (2) Methods: examines changes bacterial community available for mobilization from in-situ decomposing cowpats effects simulated rainfall. individual was monitored over 5.5 months. We used 16S rRNA metagenomics machine learning software, FEAST (Fast Expectation-mAximization microbial Source Tracking), source assignments. (3) Results: phyla Bacillota Bacteroidota are dominant microbiota fresh feces but shift Pseudomonodota, Actinomycetota, environmental aged cowpats. Potential impacts these shifts inputs local streams discussed water quality monitoring sources contamination. identified taxon orders that indicators (Oscillospirales Bacteroidales) (Peptostreptococcales-Tissierellales) bodies. (4) highlights metagenomic profiling can inform our understanding ecology communities aquatic environments ecosystem health.

Language: Английский

Citations

5
Marine sediments are identified as an environmental reservoir for Escherichia coli: comparing signature-based and novel amplicon sequencing approaches for microbial source tracking DOI Creative Commons

Ellinor M. Frank,

Jon Ahlinder,

Therese Jephson

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 907, P. 167865 - 167865

Published: Oct. 19, 2023

Viable Escherichia coli were detected in sediments near a point of wastewater discharge marine coastal environment Sweden. Since high concentrations found the nearest pipe, this suggested that treated effluent was source microbes. In order to examine hypothesis, different bioinformatics approaches applied using 16S rRNA gene V3-V4 amplicon sequences from sediments. Both signature-based tracking sequence libraries describing known sources fecal water pollution (SourceTracker); and, curated method, indicated contaminated with wastewater. The results approach independently confirmed differential abundance analysis (DESeq2). A number taxa originating identified which can be used describe contamination sediments, and spread these specific taxa, even at low relative abundance, along urban coast. Sequences phylum Bacteroidetes (such as Bacteroides Prevotella) Firmicutes Romboutsia) increased higher E. coli. addition, Trichococcus are proposed an indicator for All three approaches, detection viable coli, suggest reservoir bacteria.

Language: Английский

Citations

3
Seasonal dynamics of bacterial community structure and function in the surf zone seawater of a recreational beach in Ostend, Belgium DOI Creative Commons
Yunmeng Li, Pascal I. Hablützel, Zixia Liu

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(6)

Published: Nov. 8, 2024

Despite the importance of bacteria in surf zone water quality, detailed insights into their community composition, functions, and seasonal dynamics at recreational beaches are scarce. This study conducted year-long, weekly monitoring bacterial communities environmental factors a beach Ostend, Belgium. Using full-length 16S rRNA gene sequencing, we correlated composition predicted functions with to identify potential drivers. Bacterial were significantly affected by variations chlorophyll (Chl a), net primary productivity (NPP), seawater temperature (SWT), minimal influence from faecal inputs due human activities. Spring showed distinct abundances Planktomarina, Amylibacter, Sulfitobacter, positively Chl related sulphur oxidation potential. Summer had higher Cryomorphaceae, likely enhancing chemoheterotrophy. Beginning mid late fall extending winter, underwent substantial changes. Fall featured distinctive enrichment Thioglobaceae, inversely a. Winter was dominated Methylophilaceae (OM43 clade), negatively a, NPP, SWT. Both seasons exhibited elevated levels potentially pathogenic phenotypes methanol methylotrophy. provides baseline for understanding how respond changes impact health.

Language: Английский

Citations

0