Acid
mine
drainage
(AMD)
is
mainly
generated
by
the
oxidation
of
metal
sulfides
in
presence
artificial
disturbance
factors
such
as
mining
fissures.
The
impact
treating
AMD
pollution
mines
slavishly
replicating
traditional
treatment
technology
limited
due
to
its
large
output
and
long
duration.
Refined
identification
redox
zone
indispensable
for
remediation
mines.
In
this
study,
15
flood
dry
periods
water
samples,
borehole
samples
were
collected
hydrogeological
chemical
analysis
from
surface
groundwater
bodies
Causeway
Gully,
Dexing,
China.
storage
medium
zone,
mineral
component
study
area
finely
divided
through
vertical
medium,
components,
chemistry.
Among
them,
main
areas
with
distinct
characteristics
are
transition
zones.
degree
fracture
development
decreases
vertically
formation
depth
increases,
quartz
muscovite
display
higher
proportions
lower
parts,
while
pyrophyllite
becomes
dominant
upper
part
formation.
And
periods,
TFe
concentration
increases
first,
then
decreases,
greater
than
periods.
SO42-
root
appears
be
significantly
lesser
but
opposite
true
at
about
m.
As
a
consequence,
actual
engineering
sealing
No.
23
cave
was
carried
out
accordance
zoning
relying
on
zone.
quality
improved
after
treatment,
concentrations
TFe,
TMn,
Ca2+,
reduced.
will
serve
guide
The ISME Journal,
Journal Year:
2023,
Volume and Issue:
17(11), P. 2023 - 2034
Published: Sept. 15, 2023
Abstract
Biological
nitrogen
fixation
(BNF),
the
conversion
of
N2
into
bioavailable
(N),
is
main
process
for
replenishing
N
loss
in
biosphere.
However,
BNF
groundwater
systems
remains
poorly
understood.
In
this
study,
we
examined
activity,
abundance,
and
community
composition
diazotrophs
Hetao
Plain
Inner
Mongolia
using
15N
tracing
methods,
reverse
transcription
qPCR
(RT-qPCR),
metagenomic/metatranscriptomic
analyses.
15N2
incubation
near
situ
(9.5–585.4
nmol
L−1
h−1)
N2-fixer
enrichment
isolates
(13.2–1728.4
g−1
h−1,
as
directly
verified
by
single-cell
resonance
Raman
spectroscopy),
suggested
that
a
non-negligible
source
region.
The
expression
nifH
genes
ranged
from
3.4
×
103
to
1.2
106
copies
was
tightly
correlated
with
dissolved
oxygen
(DO),
Fe(II),
NH4+.
Diazotrophs
were
chiefly
aerobes
or
facultative
anaerobes,
dominated
Stutzerimonas,
Pseudomonas,
Paraburkholderia,
Klebsiella,
Rhodopseudomonas,
Azoarcus,
additional
uncultured
populations.
Active
diazotrophs,
which
prefer
reducing
conditions,
more
metabolically
diverse
potentially
associated
nitrification,
sulfur/arsenic
mobilization,
Fe(II)
transport,
CH4
oxidation.
Our
results
highlight
importance
subsurface
geochemical
cycles.
Aquaculture Research,
Journal Year:
2024,
Volume and Issue:
2024, P. 1 - 9
Published: May 27, 2024
Ammonia
nitrogen
is
one
of
the
major
pollutants
in
aquaculture.
However,
mechanism
ammonia
stress
on
ovarian
development
and
follicular
atresia
unknown.
In
this
study,
goldfish
(
Carassius
auratus
)
were
exposed
to
different
concentrations,
including
0
(control,
C)
50
mg/L
(high,
H)
for
48
hr
at
21
±
0.5°C.
Histology
tissue
sections
revealed
that
high
concentrations
inhibited
maturation
oocytes,
induced
broken
wrinkled
radiation
bands,
membranes,
accumulation
rod-shaped
yolk
particles.
From
transcriptome
data,
a
total
1,405
differentially
expressed
genes
(DEGs)
detected
between
H
C
groups.
Most
DEGs
highly
enriched
low-density
lipoprotein
particle-mediated
signaling,
oxidative
stress-induced
premature
senescence,
steroidogenesis
pathway.
Furthermore,
19
selected
determined
by
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR).
The
changes
antioxidant
activity
indicated
excessive
reactive
oxygen
species
(ROS)
tissue.
apoptosis
was
group.
downregulation
involved
pathway
prevented
synthesis
sexual
hormones.
These
results
suggest
high-concentration
induces
damage
goldfish,
affecting
its
function,
leading
ROS
accumulation,
apoptosis,
hindered
steroid
synthesis,
adversely
reproductive
performance.
Journal of Geophysical Research Biogeosciences,
Journal Year:
2025,
Volume and Issue:
130(2)
Published: Jan. 30, 2025
Abstract
The
seasonal
controls
of
hydrology,
temperature,
hypoxia,
and
biogeochemical
conditions
for
groundwater
ammonium–N
(NH
4
+
)
concentrations
are
not
well
understood.
Here
we
investigated
these
riparian
groundwaters
located
upstream
two
milldams
over
a
period
years.
Groundwater
chemistry
was
sampled
monthly
while
elevations,
hydraulic
gradients,
temperatures
were
recorded
sub‐hourly.
Distinct
patterns
NH
observed
which
differed
among
the
wells.
For
wells
that
displayed
strong
pattern,
increased
through
summer
peaked
in
October–November.
These
elevated
attributed
to
ammonification,
suppression
nitrification,
and/or
dissimilatory
nitrate
reduction
ammonium
(DNRA).
processes
driven
by
high
temperatures,
low
gradients
(or
long
residence
times),
hypoxic/anoxic
conditions,
availability
dissolved
organic
carbon
as
an
electron
donor.
In
contrast,
decreased
from
January
April
during
cool
wet
conditions.
A
with
total
iron
(TdFe)
had
but
muted
response.
addition
hydrologic
controls,
this
response
additional
contribution
Fe‐driven
autotrophic
DNRA
ammonification
linked
Fe
reduction.
Understanding
temporal
factors
controlling
is
important
making
appropriate
watershed
management
decisions
implementing
best
practices.
