Authorea (Authorea),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 16, 2024
not-yet-known
unknown
A
novel
Pseudomonas
aeruginosa
phage,
designated
as
PaYy-2,
has
been
isolated
from
a
lake
in
China
and
its
genome
meticulously
sequenced
characterized.
The
PaYy-2
phage
possesses
92,348
base
pair
double-stranded
DNA
that
encodes
for
168
proteins,
exhibiting
68%
similarity
to
the
of
known
YS35.
Phylogenetic
analysis
reveals
putative
RNA
polymerase
shares
94%
with
SRT6,
terminase
large
subunit
exhibits
96%
resemblance
YS35,
both
which
are
classified
under
Pakpunavirus
genus.
Morphological
examination
via
transmission
electron
microscopy
(TEM)
provided
insights
into
phage's
tail
particle
morphology.
These
collective
findings
underscore
classification
distinct
species
within
genus
family
Myoviridae.
PLoS Biology,
Journal Year:
2024,
Volume and Issue:
22(8), P. e3002746 - e3002746
Published: Aug. 7, 2024
Understanding
the
dynamic
evolution
of
Salmonella
is
vital
for
effective
bacterial
infection
management.
This
study
explores
role
flexible
genome,
organised
in
regions
genomic
plasticity
(RGP),
shaping
pathogenicity
lineages.
Through
comprehensive
analysis
12,244
spp.
genomes
covering
2
species,
6
subspecies,
and
46
serovars,
we
uncover
distinct
integration
patterns
pathogenicity-related
gene
clusters
into
RGP,
challenging
traditional
views
distribution.
These
RGP
exhibit
preferences
specific
spots,
presence
or
absence
such
spots
across
lineages
profoundly
shapes
strain
pathogenicity.
are
guided
by
conserved
flanking
genes
surrounding
implicating
their
involvement
regulatory
networks
functional
synergies
with
integrated
clusters.
Additionally,
emphasise
multifaceted
contributions
plasmids
prophages
to
diverse
Overall,
this
provides
a
blueprint
potential
.
unique
insight
identifies
nonpathogenic
that
hold
harbouring
genes,
providing
foundation
predicting
future
adaptations
developing
targeted
strategies
against
emerging
human
pathogenic
strains.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(8), P. 3895 - 3907
Published: Feb. 15, 2024
Volatilization
of
lower-chlorinated
polychlorinated
biphenyls
(LC-PCBs)
from
sediment
poses
health
threats
to
nearby
communities
and
ecosystems.
Biodegradation
combined
with
black
carbon
(BC)
materials
is
an
emerging
bioaugmentation
approach
remove
PCBs
sediment,
but
development
aerobic
biofilms
on
BC
for
long-term,
sustained
LC-PCBs
remediation
poorly
understood.
This
work
aimed
characterize
the
cell
enrichment
activity
biphenyl-
benzoate-grown
Paraburkholderia
xenovorans
strain
LB400
various
BCs.
Biphenyl
dioxygenase
gene
(bphA)
abundance
four
types
demonstrated
corn
kernel
biochar
hosted
at
least
4
orders
magnitude
more
attached
cells
per
gram
than
other
feedstocks,
microscopic
imaging
revealed
live
fraction
was
>1.5×
GAC.
characteristics
(i.e.,
sorption
potential,
pore
size,
pH)
appear
contribute
attachment
differences.
Reverse
transcription
qPCR
indicated
that
feedstocks
significantly
influenced
bphA
expression
in
cells.
The
transcript-per-gene
ratio
>10-fold
suspended
cells,
confirmed
by
transcriptomics.
RNA-seq
also
significant
upregulation
biphenyl
benzoate
degradation
pathways
as
well
revealing
biofilm
formation
potential/cell–cell
communication
pathways.
These
novel
findings
demonstrate
PCB-degrading
could
be
tuned
adjusting
feedstocks/attributes
improve
biodegradation
potential.
Environment International,
Journal Year:
2024,
Volume and Issue:
187, P. 108659 - 108659
Published: April 24, 2024
Quorum-sensing
bacteria
(QSB)
are
crucial
factors
for
microbial
communication,
yet
their
ecological
role
in
wastewater
treatment
plants
(WWTPs)
remains
unclear.
Here,
we
developed
a
method
to
identify
QSB
by
comparing
16S
rRNA
gene
sequences.
388
activated
sludge
samples
collected
from
130
WWTPs
across
China
primarily
were
identified
as
rare
taxa
and
conditionally
taxa.
A
co-occurrence
network
shared
all
communities
revealed
that
exhibited
higher
average
clustering
coefficient
(0.46)
than
non-QSB
(0.15).
Individual
networks
demonstrated
quorum
sensing
microbiomes
positively
correlated
with
robustness
complexity,
including
link
density.
We
confirmed
keystones
nodes
have
positive
impact
on
complexity
influencing
modularity
through
structural
equation
model.
Meanwhile,
directly
contributed
maintaining
(r
=
0.29,
P
<
0.05).
Hence,
play
an
important
promoting
stability.
Furthermore,
associated
the
functional
composition
of
0.33,
0.01),
especially
denitrification
capacity
0.45,
0.001).
Overall,
elucidated
significance
provided
support
QS-based
regulation
communities.
