Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 370, P. 122497 - 122497

Published: Sept. 14, 2024

The recycling of food waste (FW) through anaerobic fermentation into lactic acid (LA), with two isomers L-LA and D-LA, aligns the principles a bio-based circular economy. However, FW is often limited by competing pathways, acidification inhibition, trace metals deficiency. This study investigates introduction landfill leachate, containing buffering agents (ammonia) metals, fermentation. Various dosages ranging from 90 (LN-90) to 450 mg/L (LN-450) based on inclusive ammonia calculation, were employed. Results showed that LA production peaked at 43.65 ± 0.57 g COD/L in LN-180 day 6, high optical activity 92.40 1.15 %. Fermentation pathway analysis revealed leachate amendment enhances hydrolysis (as evidenced increased amylase, α-glucosidase, protease) glycolysis (resulting enhanced utilization carbohydrates glucose). plays crucial role as buffer, maintaining optimal pH conditions (5-7), thereby reducing volatile fatty thus intensifying orientations. L-lactate dehydrogenase (L-LA generation) decreased NAD-independent lactate (LA consumption) properly dosed further explained accumulation L-LA. Dominance bacteria, including Streptococcus, Enterococcus, Klebsiella, Bifidobacterium, Bavariicoccus, Lacticaseibacillus, accounted for 91.08% (LN-90), while inhibitory effects observed LN-450 (4.45%). Functional gene supported glycolysis, dehydrogenase, nitrogen assimilation. Finally, network indicates beneficial effect genus Enterococcus Klebsiella addition. demonstrates efficiency utilizing enhance fermentation, aligning concept economy transforming valuable resources.

Language: Английский