Low-volume enrichment method supports high throughput bacteriophage screening and isolation from wastewater DOI Creative Commons
Patrick Kenney, Óscar G. Gómez-Duarte

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0298833 - e0298833

Published: April 16, 2024

Bacteriophage therapy is a rapidly growing field of study. Narrow host ranges, bacterial resistance, and limited antibiotic availability make lytic phages feasible therapeutic potential. Phage discovery, critical step in developing phage therapy, pathway to accessible treatment. This has always been laborious, time-consuming resource-intensive process. In this paper, we describe 96-well plate low-volume bacteriophage enrichment method with concentrated environmental sources discover isolate targeting multiple organisms simultaneously. Samples from natural water sources, wastewater influent, activated sludge were tested large volume cultures format. Each the capacity run as many 48 different combinations hosts. The time identify presence sample was 5 10 hours format versus minimum 2 days traditional method. labor expense involved also favor There some loss discovered using technique, primarily species less prevalent environment. an easily modifiable that amenable automation variety potential sources.

Language: Английский

Simultaneous removal of NOM and sulfate in a bioelectrochemical integrated biofilter treating reclaimed water DOI
Lei Xu, Shian Song,

Nigel Graham

et al.

Water Research, Journal Year: 2024, Volume and Issue: 252, P. 121193 - 121193

Published: Jan. 25, 2024

Language: Английский

Citations

6
Climate adaptation and resilience of biofiltration as a low-cost technological solution for water treatment – A critical review DOI Creative Commons
Abdou Ali, Oliver Terna Iorhemen, Ronald W. Thring

et al.

Cleaner Water, Journal Year: 2025, Volume and Issue: unknown, P. 100062 - 100062

Published: Jan. 1, 2025

Language: Английский

Citations

0
Neglected role of virus-host interactions driving antibiotic resistance genes reduction in an urban river receiving treated wastewater DOI
Xiaojie Yan, Yuan Xin, Liying Zhu

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123627 - 123627

Published: April 1, 2025

Language: Английский

Citations

0
Low-volume enrichment method supports high throughput bacteriophage screening and isolation from wastewater DOI Creative Commons
Patrick Kenney, Óscar G. Gómez-Duarte

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0298833 - e0298833

Published: April 16, 2024

Bacteriophage therapy is a rapidly growing field of study. Narrow host ranges, bacterial resistance, and limited antibiotic availability make lytic phages feasible therapeutic potential. Phage discovery, critical step in developing phage therapy, pathway to accessible treatment. This has always been laborious, time-consuming resource-intensive process. In this paper, we describe 96-well plate low-volume bacteriophage enrichment method with concentrated environmental sources discover isolate targeting multiple organisms simultaneously. Samples from natural water sources, wastewater influent, activated sludge were tested large volume cultures format. Each the capacity run as many 48 different combinations hosts. The time identify presence sample was 5 10 hours format versus minimum 2 days traditional method. labor expense involved also favor There some loss discovered using technique, primarily species less prevalent environment. an easily modifiable that amenable automation variety potential sources.

Language: Английский

Citations

2