Differential interaction modes of As(III)/As(V) with microbial cell membrane induces opposite effects on organic contaminant biodegradation in groundwater DOI Creative Commons

Wenbo Guo,

Deping Li,

Ying Zhai

et al.

Environment International, Journal Year: 2024, Volume and Issue: 193, P. 109074 - 109074

Published: Oct. 14, 2024

Arsenic, a widespread toxic metalloid in groundwater, derives both from natural geological environment and industrial discharge, is extensively detected to be coexisting with organic contaminants, such as 2,4,6-trichlorophenol (TCP), prior concerned pollutant. During biological remediation of arsenic potentially intervenes microbial behaviors. This study found an opposite interference its two different valences (III V) on the degradation TCP by functional bacteria, Sphingomonas fennica K101. As(III) inhibited concentration-dependent manner (from 0.1-10 mg/L), maximum inhibition rate 35.5%, whereas As(V) exhibited promoting effects 13.8% 33.2% at 1 mg/L 10 mg/L, respectively. Employing field emission transmission electron microscopy, quantum chemical calculations, fourier-transform ion cyclotron resonance mass spectrometry metabolomic analysis, we unveil distinct interactions between cell membranes valence states. Exposure led significant accumulation cytoplasm, followed interaction intracellular ferritin (ferritin heavy chain 1), releasing iron ions generating ROS. Subsequently, it induced ferroptosis disrupted bacterial basal metabolism, thereby inhibiting biodegradation. Oppositely, bound critical component sphingosine triggered polymerization, increasing membrane permeability, which was evidenced measuring lactate dehydrogenase release. process facilitated transmembrane permeation reducing or extracellular secretion resistance. concurrently upregulated energy metabolism accelerated degradation. Our elucidates influence prevalent biodegradation efficacy, particularly amidst changing redox conditions associated varying valences.

Language: Английский

Removal of sulfamethoxazole in an algal-bacterial membrane aerated biofilm reactor: microbial responses and antibiotic resistance genes DOI

Haijing Ren,

Rongchang Wang,

Luyao Ying

et al.

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122595 - 122595

Published: Oct. 11, 2024

Language: Английский

Citations

6
Perspectives on adhesion and irreversible fouling to delineate mitigation effects of autoinducer-2 mediated quorum quenching on biofouling DOI
Yuan Li, Danchen Zhu,

Le Van Hong

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: 426, P. 132380 - 132380

Published: March 10, 2025

Language: Английский

Citations

0
Cell Response to Nanoplastics and Their Carrier Effects Tracked Real-Timely with Machine Learning-Driven Smart Surface-Enhanced Raman Spectroscopy Slides DOI
Ruili Li,

Shu-Ting Huang,

Yuyang Hu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Research on nanoplastic (NP) toxicity and their "carrier effects" human health remains nascent, especially for real-time, in situ monitoring of metabolic reactions live cells. Herein, we developed smart surface-enhanced Raman spectroscopy (SERS) slides using a cyclic centrifugation-enhanced electrostatic loading (CCEL) method to facilitatively track live-cell signals. The designed core-shell polystyrene NPs (mPS) with embedded probes successfully identified intracellular accumulation via distinct Raman-silent peak. SERS slide effectively monitored the changes induced by mPS at molecular level, distinguishing different stages membrane interaction, endocytosis process, endosomal aggregation, cell apoptosis. Besides, this platform was employed perform comparison cycle alterations bare effects", revealing that extended both S G2 phases BEAS-2B cells, while further prolonged disrupted S-phase progression. Additionally, integrated machine learning algorithms accurately predict impacts associated effects". This study provides label-free, situ, real-time NP-induced laying groundwork investigation into cytotoxic behaviors strategies mitigate NP toxicity.

Language: Английский

Citations

0
Nanoplastics Pre-exposure to Microbial Consortium Influencing Their Ability to Degrade Pollutants: “Stagnation Effect” and “Self-recovery” DOI
Wenbo Guo, Xiaoyu Ma, Hui Yu

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123642 - 123642

Published: April 1, 2025

Language: Английский

Citations

0
Deciphering membrane biofouling induced by micro-/nano-plastics in nanofiltration: Metagenomic insights and spacer-driven mitigations DOI
Xinhui Liu,

Yu Yang,

Nigel Graham

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123682 - 123682

Published: April 1, 2025

Language: Английский

Citations

0
Differential interaction modes of As(III)/As(V) with microbial cell membrane induces opposite effects on organic contaminant biodegradation in groundwater DOI Creative Commons

Wenbo Guo,

Deping Li,

Ying Zhai

et al.

Environment International, Journal Year: 2024, Volume and Issue: 193, P. 109074 - 109074

Published: Oct. 14, 2024

Arsenic, a widespread toxic metalloid in groundwater, derives both from natural geological environment and industrial discharge, is extensively detected to be coexisting with organic contaminants, such as 2,4,6-trichlorophenol (TCP), prior concerned pollutant. During biological remediation of arsenic potentially intervenes microbial behaviors. This study found an opposite interference its two different valences (III V) on the degradation TCP by functional bacteria, Sphingomonas fennica K101. As(III) inhibited concentration-dependent manner (from 0.1-10 mg/L), maximum inhibition rate 35.5%, whereas As(V) exhibited promoting effects 13.8% 33.2% at 1 mg/L 10 mg/L, respectively. Employing field emission transmission electron microscopy, quantum chemical calculations, fourier-transform ion cyclotron resonance mass spectrometry metabolomic analysis, we unveil distinct interactions between cell membranes valence states. Exposure led significant accumulation cytoplasm, followed interaction intracellular ferritin (ferritin heavy chain 1), releasing iron ions generating ROS. Subsequently, it induced ferroptosis disrupted bacterial basal metabolism, thereby inhibiting biodegradation. Oppositely, bound critical component sphingosine triggered polymerization, increasing membrane permeability, which was evidenced measuring lactate dehydrogenase release. process facilitated transmembrane permeation reducing or extracellular secretion resistance. concurrently upregulated energy metabolism accelerated degradation. Our elucidates influence prevalent biodegradation efficacy, particularly amidst changing redox conditions associated varying valences.

Language: Английский

Citations

0