Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 10, 2025
Abstract
This
study
investigates
the
performance
of
a
hydrogen-based
membrane
biofilm
reactor
(MBfR)
for
nitrogen
transformation
over
an
extended
operational
period
exceeding
200
days.
During
monitoring,
sustained
highly
alkaline
conditions
with
pH
consistently
11.20,
while
achieving
ammonia
conversion
efficiency
above
60%
and
maintaining
nitrite
accumulation
rate
below
1%.
Integrated
mass
balance
calculations
microbial
community
profiling
revealed
coexistence
denitrification
dissimilatory
nitrate
reduction
to
ammonium
(DNRA)
pathways
within
network.
A
factorial
experimental
design
was
implemented
hydraulic
retention
time
(HRT)
influent
concentration
as
independent
variables,
generating
nine
distinct
regimes
through
cross-variable
permutations.
Subsequent
analysis
extensive
datasets
enabled
development
Response
Surface
Methodology
(RSM)
model
simulate
dynamics.
Model
validation
confirmed
statistical
reliability
predictive
accuracy
RSM
framework.
Critical
demonstrated
that
in
hydrogen
autotrophic
system
exhibited
significant
correlation
individual
variables
themselves
but
showed
negligible
dependence
on
their
interactive
effects.
These
findings
provide
novel
insights
into
process
optimization
strategies
theoretical
understanding
convention.
