Biotransformation of 6:2/4:2 fluorotelomer alcohols by Dietzia aurantiaca J3: Enzymes and proteomics

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135510 - 135510

Published: Aug. 13, 2024

Per- and polyfluoroalkyl substances (PFAS) are recalcitrant synthetic organohalides known to negatively impact human health. Short-chain fluorotelomer alcohols considered the precursor of various perfluorocarboxylic acids (PFCAs) in environment. Their ongoing production widespread detection motivate investigations their biological transformation. Dietzia aurantiaca strain J3 was isolated from PFAS-contaminated landfill leachate using 6:2 sulphonate (6:2 FTS) as a sulphur source. Resting cell experiments were used test if could transform 4:2 FTOH). Strain transformed into PFCAs, polyfluorocarboxylic transient intermediates. Over 6 days, 80 % 58 FTOH (0.1 mM) (0.12 degraded with 6.4 14 fluoride recovery respectively. Fluorotelomer unsaturated carboxylic acid FTUCA) most abundant metabolite, accounting for 21 30 mol% (0.015 applied on day zero. Glutathione (GSH) conjugates 6:2/4:2 5:3 FTCA adducts also structurally identified. Proteomics studies conducted identify enzymes biotransformation pathway have revealed role involved β oxidation. This is first report glutathione prokaryotes, study explore by pure bacterial strain.

Language: Английский

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Cascading Pathways Regulate the Biotransformations of Eight Fluorotelomer Acids Performed by Wastewater Microbial Communities

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Polyfluoroalkyl substances can be biotransformed in natural or engineered environmental systems to generate perfluoroalkyl acids (PFAAs). Data are needed support the development of biotransformation pathway prediction tools that simulate pathways polyfluoroalkyl specific systems. The goal this study was experimentally evaluate eight structurally similar fluorotelomer identify products and propose pathways. We selected six carboxylic two sulfonic employed a test system which batch reactors seeded with aerobic wastewater microbial communities. identified 111 among parent compounds, 58 represent unique chemical structures. Many result apparent dehydrogenation, monohydroxylation, alcohol oxidation, decarboxylation, HF-elimination, reductive defluorination biotransformations. use these data cascading regulated by integrated synergistic α-oxidation-like, β-oxidation-like, biotransformations formation terminal PFAAs varying chain length. Our provide comprehensive view on our results used ongoing tools.

Language: Английский

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Enriching Fluorotelomer Carboxylic Acids-Degrading Consortia from Sludges and Soils

Published: Aug. 30, 2024

Fluorotelomer carboxylic acids (FTCAs) has drawn increasing attention due to their prevalent occurrence, high toxicity, and bioaccumulating effects. In this study, we enriched characterized microbial consortia with sustainable FTCA removal abilities from two activated sludges five soils when no external carbon sources were supplemented. After four generations of enrichment, stable 6:2 5:3 biodegradation achieved, reaching 0.72~0.98 0.53~1.05 µM/day, respectively. Coupling biotransformation, fluoride release co-occurred, conducive approximate 0.19 per molecule that was biodegraded. contrast, minimal free detected in FTCA-amended consortia, indicating the dominance “non-fluoride releasing pathways”. Microbial community analysis revealed 13 genera across all consortia. Among them, 3 genera, including Hyphomicrobium, Methylorubrum, and Achromobacter, found more amended than those an identical inoculation source, suggesting involvement biodefluorination. This study uncovered can degrade FTCAs without supplement sources, though low biotransformation biodefluorination rates. Further research is underscored investigate involved pathways mechanisms, as well effects sources.

Language: Английский

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Microbial Remediation of Fluoride-Contaminated Water

Environmental science and engineering, Journal Year: 2024, Volume and Issue: unknown, P. 255 - 282

Published: Dec. 31, 2024

Language: Английский

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