PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0312157 - e0312157

Published: Nov. 6, 2024

Wastewater treatment plants (WWTPs) receive wastewater from various sources. Despite aiming to remove contaminants, microplastics persist. Plastic surfaces are quickly colonized by microbial biofilm (“plastispheres”). Plastisphere communities suggested promote the spread and survival of potential human pathogens, suggesting that transfer plastispheres environment could pose a risk environmental health. The study aimed identify pathogens in plastispheres, specifically food-borne addition characterizing taxonomic diversity composition plastispheres. Plastispheres accumulated on polypropylene (PP), polyvinyl chloride (PVC), high-density polyethylene propylene (HDPE) exposed raw treated were analyzed via cultivation methods, quantitative reverse transcription PCR (RT‒qPCR) 16S rRNA amplicon sequencing. RT‒qPCR revealed presence foodborne pathogenic bacteria viruses, such as Listeria monocytogenes , Escherichia coli norovirus, adenovirus. Viable isolates emerging species Klebsiella pneumoniae Acinetobacter spp. identified wastewater, indicating might survive during treatment. These findings underscore harbor disseminate species, posing challenges water reuse initiatives. explored through sequencing, significantly influenced duration time plastic spent wastewater. In contrast, specific material did not influence bacterial composition, while was affected. Without efficient proper waste management, act source transferring plastic-associated into food chain possibly threat Continued research innovation essential improve removal associated microorganisms

Language: Английский