Maximizing viral nucleic acid yield from passive samplers: Evaluating elution and extraction protocols DOI Creative Commons
Madison Gouthro, Emalie K. Hayes, Graham A. Gagnon

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177834 - 177834

Published: Nov. 30, 2024

The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their ability recover SARS-CoV-2 bacteriophage MS2 from samplers. Promega Wizard® Enviro Total Nucleic Acid Kit, paired with a Tween®20-based buffer, provided highest virus efficiency sampling. Field-scale applications demonstrated effectiveness samplers capturing SARS-CoV-2, INFA, RSV, MeV using optimized protocols. combination Tween®20 based buffer kit led increased detection frequencies grab samples, which remained lower than underscores importance selecting appropriate TNA maximize samples. By optimizing these protocols, we enhance sensitivity reliability ecosystems.

Language: Английский

Assessing Passive Sampling for the Monitoring of E. coli and Cryptosporidium spp. in Environmental Waters DOI

Ilya Law,

Erin L. Becker,

Brandon S. Spoja

et al.

ACS ES&T Water, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Passive sampling has shown promise as an alternative approach for monitoring of pathogens in aquatic matrices. We conducted two controlled experiments to compare the efficacy membrane passive composite both wastewater and surface water detection Escherichia coli Cryptosporidium. also investigated relative uptake E. Cryptosporidium onto samplers over time. Both methods returned positive detections at all deployment times (4, 8, 24, 48, 72, 96 h) showed similar rates samples (31% passive; 41% composite) (76% 86% composite). found significant linear up h (R2 = 0.932; p 0.002). In wastewater, maximum sampler was reached after 24 h. For Cryptosporidium, observed 0.805; 0.015) 0.877; 0.006). Our results support that may be used waters

Language: Английский

Citations

0
Maximizing viral nucleic acid yield from passive samplers: Evaluating elution and extraction protocols DOI Creative Commons
Madison Gouthro, Emalie K. Hayes, Graham A. Gagnon

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177834 - 177834

Published: Nov. 30, 2024

The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their ability recover SARS-CoV-2 bacteriophage MS2 from samplers. Promega Wizard® Enviro Total Nucleic Acid Kit, paired with a Tween®20-based buffer, provided highest virus efficiency sampling. Field-scale applications demonstrated effectiveness samplers capturing SARS-CoV-2, INFA, RSV, MeV using optimized protocols. combination Tween®20 based buffer kit led increased detection frequencies grab samples, which remained lower than underscores importance selecting appropriate TNA maximize samples. By optimizing these protocols, we enhance sensitivity reliability ecosystems.

Language: Английский

Citations

0