Phages-bacteria interactions underlying the dynamics of polyhydroxyalkanoate-producing mixed microbial cultures via meta-omics study

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT The dynamics of the structure polyhydroxyalkanoate-producing mixed microbial cultures (PHA-MMCs) during enrichment and maintenance is an unsolved problem. effect phages has been proposed as a cause dynamic changes in community structure, but evidence lacking. To address this question, five PHA-MMCs were enriched, biological samples sampled temporally to study interactions between phage bacterial members by combining metagenomics metatranscriptomics. A total 963 metagenome-assembled genomes (MAGs) 4,294 operational taxonomic units (pOTUs) assembled from bulk metagenomic data. communities remarkably consistent. Structural equation modeling analysis showed that could infect lyse dominant species vacate ecological niches for other species, resulting succession state which alternated. Seven key auxiliary metabolic genes (AMGs), phaC , fadJ acs ackA phbB acdAB, fadD potentially contributing PHA synthesis identified sequences. Importantly, these AMGs transcribed, indicating they active expression state. meta-analysis provides first catalog carry, well how affect communities. This reference subsequent studies on understanding regulating open systems. IMPORTANCE biodegradable plastic organic waste through (PHA-MMCs), at extremely low cost, potential expanded production. However, are poorly understood. Our results demonstrate time impact PHA-MMCs. There complex producers (e.g., Azomonas Paracoccus Thauera ) Casadabanvirus unclassified Hendrixvirinae). Phage can regulate activity In addition, related may hitchhike phage-host infection cycles, enabling their dissemination across communities, act critical genetic reservoir members, facilitating access synthesis-related functional traits. highlights suggesting have be used tool better controlling

Language: Английский

Regulatory Landscape and the Potential of Bacteriophage Applications in the United States’ Food Industry

Journal of Food Protection, Journal Year: 2025, Volume and Issue: unknown, P. 100510 - 100510

Published: April 1, 2025

Bacteriophages are promising tools for mitigating bacterial contamination because of their specificity cells. The food industry can enhance safety and quality by applying bacteriophages in various settings, from farms to ready-to-eat (RTE) products. Renewed interest has been driven recent advancement research the increasing need sustainable alternatives safety. However, understanding regulatory requirements using agriculture United States is essential. These complex varied regulations impact approval implementation bacteriophage-based solutions. Addressing these crucial ensure that bacteriophage applications comply legal standards. This review synthesizes evolving framework governing use industry, providing a clear evaluation processes, while highlighting changes addressing challenges opportunities effective integration into processing.

Language: Английский