A six-plex digital PCR assay for monitoring respiratory viruses in wastewater DOI Creative Commons

Melissa Pitton,

Rachel E. McLeod, Lea Caduff

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

ABSTRACT Wastewater-based surveillance systems can track trends in multiple pathogens simultaneously by leveraging efficient, streamlined laboratory processing. In Switzerland, wastewater is conducted for fourteen locations representing 2.3 million people, or 26% of the national population, with simultaneous four respiratory pathogens. Trends diseases are tracked using a novel, six-plex digital PCR assay targeting Influenza A, B, Respiratory Syncytial Virus, and SARS-CoV-2 N1 N2 genes, as well Murine Hepatitis Virus recovery efficiency control. The multiplex was developed to ensure sensitivity accurate quantification all targets simultaneously. Wastewater data also integrated disease obtained through both mandatory reporting system Swiss Sentinel System (Sentinella), voluntary general practitioners. Comparisons between case from July 2023 2024 demonstrate high level agreement, specifically SARS-CoV-2, Virus. Lower correspondence observed which highlights challenges tracking dynamics during seasons without pronounced outbreak periods. monitoring further revealed that gene led divergent estimates SARS-Cov-2 viral loads, highlighting impact mutations target region on trends. study emphasizes importance an program complementary tool public health demonstrating clear concordance clinical beyond SARS-CoV-2.

Language: Английский

COVID-19 Transmission During the Winter 2023-24 Surge: A Comparative Analysis of Surveillance Estimates in the U.S., Canada, and the U.K. DOI Creative Commons
Michael Hoerger, James Gerhart,

Tristen Peyser

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Background: Better estimates of COVID-19 transmission are needed since testing has declined. The present investigation examined the correspondence among during winter 2023-24 surge using wastewater-derived for U.S. and Canada testing-derived estimate in U.K. to evaluate validity provide vital public health data on levels. Methods: study used from (Pandemic Mitigation Collaborative dashboard) (COVID-19 Resources testing-based surveillance (Health Security Agency). Data sets were linked by date relative peak within each set. Analyses focused UKHSA period November 2023 March 2024. 1) described day, 2) agreement patterns via correlations, 3) absolute proportion population actively infectious across two months transmission, 4) populations infected months. Results: On day infections, an estimated 1.95 million people U.S., 148 thousand Canada, 431 U.K., meaning 2.5%-4.5% these infectious. Estimates showed high throughout wave, especially between (r=.974, p<.001). During 93.5% 68.8% had excellent or better with data. An >100 months, 20.9%-26.0% population. Discussion: Findings support ongoing significance documenting levels surge. Transmission methodologies nations. More resources prevent diagnose treat long-term sequelae.

Language: Английский

Citations

1
A six-plex digital PCR assay for monitoring respiratory viruses in wastewater DOI Creative Commons

Melissa Pitton,

Rachel E. McLeod, Lea Caduff

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

ABSTRACT Wastewater-based surveillance systems can track trends in multiple pathogens simultaneously by leveraging efficient, streamlined laboratory processing. In Switzerland, wastewater is conducted for fourteen locations representing 2.3 million people, or 26% of the national population, with simultaneous four respiratory pathogens. Trends diseases are tracked using a novel, six-plex digital PCR assay targeting Influenza A, B, Respiratory Syncytial Virus, and SARS-CoV-2 N1 N2 genes, as well Murine Hepatitis Virus recovery efficiency control. The multiplex was developed to ensure sensitivity accurate quantification all targets simultaneously. Wastewater data also integrated disease obtained through both mandatory reporting system Swiss Sentinel System (Sentinella), voluntary general practitioners. Comparisons between case from July 2023 2024 demonstrate high level agreement, specifically SARS-CoV-2, Virus. Lower correspondence observed which highlights challenges tracking dynamics during seasons without pronounced outbreak periods. monitoring further revealed that gene led divergent estimates SARS-Cov-2 viral loads, highlighting impact mutations target region on trends. study emphasizes importance an program complementary tool public health demonstrating clear concordance clinical beyond SARS-CoV-2.

Language: Английский

Citations

0