Protocol to identify biomarkers in patients with post-COVID condition using multi-omics and machine learning analysis of human plasma

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(2), P. 103041 - 103041

Published: April 27, 2024

Here, we present a workflow for analyzing multi-omics data of plasma samples in patients with post-COVID condition (PCC). Applicable to various diseases, outline steps preprocessing and integrating diverse assay datasets. Then, detail statistical analysis unveil profile changes identify biomarker-clinical variable associations. The last two discuss machine learning techniques unsupervised clustering based on their inherent molecular similarities feature selection predictive biomarkers. For complete details the use execution this protocol, please refer Wang et al.

Language: Английский

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Low-Grade Inflammation in Long COVID Syndrome Sustains a Persistent Platelet Activation Associated With Lung Impairment

JACC Basic to Translational Science, Journal Year: 2024, Volume and Issue: 10(1), P. 20 - 39

Published: Nov. 27, 2024

In the present study, we provide evidence on potential mechanisms involved in residual pulmonary impairment described long COVID syndrome. Data highlight that lung damage is significantly associated with a proinflammatory platelet phenotype, characterized mainly by formation of platelet-leukocyte aggregates. ex vivo experiments, plasma reproduces activation observed and highlights low-grade inflammation as underpinning mechanism, exploiting synergistic activity between C-reactive protein subthreshold concentrations interleukin-6. The platelet-activated phenotype blunted anti-inflammatory antiplatelet drugs, suggesting therapeutic option this clinical setting.

Language: Английский

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COVIDomics: Metabolomic Views on COVID-19

Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 702 - 702

Published: Dec. 12, 2024

During the COVID-19 pandemic, omics-based methodologies were extensively used to study pathological mechanisms of SARS-CoV-2 infection and replication in human cells at a large scale [...]

Language: Английский

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Prospectively Defined Clusters of COVID-19 Sequelae

The Journal of Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Post-COVID conditions (PCC)" or "Long COVID" are used to describe the post-acute sequelae of SARS-CoV-2 infection, but this represents a hugely heterogeneous patient population, one that is characterized by lack single defining pathology.Hypotheses explain its chameleonic symptoms include immune response persistent viral antigens, development autoimmunity, dysbiosis, latent virus reactivation, unrepaired tissue damage, and subsequent broad perturbation, name few (1).As most recent data, at least 10% patients with severe acute COVID-19 infections experience (1), generally described persistence beyond initial phase illness can last from weeks months, sometimes even years.The PCC, often overlapping, range impairment physical, cognitive, multiple organ domains, causing an overall decline in quality life.While many individuals report loss smell taste, fatigue, brain fog, joint pain, respiratory issues, due heterogeneity scope symptoms, it has been difficult determine definition (1, 2).The U.S. Centers Disease Control Prevention (CDC) defines PCC as persisting for 28 days more after infection (3), whereas National Institute Health Care Excellence U.K. (NICE) World Organization (WHO) agree continuation must be 3 months classified (4, 5).In further support, Academies Sciences, Engineering, Medicine (NASEM) "an infection-associated chronic

Language: Английский

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Long COVID Clinical Evaluation, Research and Impact on Society: A Global Expert Consensus

Published: Jan. 1, 2024

Language: Английский

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