Characterization of the Pathogenic Features of Multiple SARS‐CoV‐2 Pandemic Strains in Different Mouse Models DOI

Huize Sun,

Kunpeng Liu,

Baocheng Yu

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(11)

Published: Nov. 1, 2024

ABSTRACT Elucidating the detailed features of emerging SARS‐CoV‐2 strains both in vitro and vivo is indispensable for development effective vaccines or drugs against viral infection. We thoroughly characterized virological pathogenic eight different pandemic strains, from WT strain to current circulating sublineage EG.5.1, vivo. Besides observed Vero E6 cells, Omicron variants, BA.1 exhibited enhanced infectious effects upper respiratory tract K18 human angiotensin‐converting enzyme (ACE2) (K18 hACE2) transgenic mice. Both XBB.1.9.1 EG.5.1 presented stronger tropism brain, which could be main reason increased lethal on In addition, pathogenesis comparisons among all these viruses C57BL/6JGpt mice indicated that variant two new sublineages possessed dual tropisms mice, were further confirmed by subsequent bioinformatic analyses actual affinity comparison between RBDs mouse receptor ACE2. Furthermore, immunocompromised BKS‐db found more susceptible compared revealed infectivity was determined its host immunocompetence. Thus, this study not only contributes a systematic understanding but also provides insights combat potential future surges variants.

Language: Английский

Advance in peptide-based drug development: delivery platforms, therapeutics and vaccines DOI Creative Commons
Wen‐Jing Xiao, Wenjie Jiang, Zheng Chen

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 5, 2025

The successful approval of peptide-based drugs can be attributed to a collaborative effort across multiple disciplines. integration novel drug design and synthesis techniques, display library technology, delivery systems, bioengineering advancements, artificial intelligence have significantly expedited the development groundbreaking drugs, effectively addressing obstacles associated with their character, such as rapid clearance degradation, necessitating subcutaneous injection leading increasing patient discomfort, ultimately advancing translational research efforts. Peptides are presently employed in management diagnosis diverse array medical conditions, diabetes mellitus, weight loss, oncology, rare diseases, additionally garnering interest facilitating targeted platforms advancement vaccines. This paper provides an overview present market clinical trial progress therapeutics, platforms, It examines key areas through literature analysis emphasizes structural modification principles well recent advancements screening, design, technologies. accelerated including peptide-drug complexes, new vaccines, innovative diagnostic reagents, has potential promote era precise customization disease therapeutic schedule.

Language: Английский

Citations

2
Intranasally administrated fusion-inhibitory lipopeptides block SARS-CoV-2 infection in mice and enable long-term protective immunity DOI Creative Commons
Saïd Mougari, Valérie Favède, Camilla Predella

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 15, 2025

Abstract We have assessed antiviral activity and induction of protective immunity fusion-inhibitory lipopeptides derived from the C-terminal heptad-repeat domain SARS-CoV-2 spike glycoprotein in transgenic mice expressing human ACE2 (K18-hACE2). The block infection cell lines lung-derived organotypic cultures. Intranasal administration allows maintenance homeostatic transcriptomic immune profile lungs, prevents body-weight loss, decreases viral load shedding, protects death caused by variants. Prolonged high-dose has neither adverse effects nor impairs peptide efficacy subsequent challenges. peptide-protected develop cross-reactive neutralizing antibodies against both used for initial recently circulating variants, are completely protected a second lethal infection, suggesting that they developed SARS-CoV-2-specific immunity. This strategy provides an additional approach global effort COVID-19 may contribute to development rapid responses emerging pathogenic viruses.

Language: Английский

Citations

1
Synergistic peptide combinations designed to suppress SARS-CoV-2 DOI Creative Commons
Tao Han,

Linhong Song,

Xinxin Niu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30489 - e30489

Published: April 29, 2024

The SARS-CoV-2, responsible for the COVID-19 pandemic, poses a significant threat to global healthcare. Peptide and peptide-based inhibitors, known their safety, efficacy, selectivity, have recently emerged as promising candidates treating late-developing viral infections. In this study, three peptides were selected target different stages of invasion, specifically ACE2 S protein binding, well membrane fusion. objective was assess ability impede entry SARS-CoV-2 Spike pseudotyped virus. Our findings revealed that combination these demonstrated enhanced antiviral effects. This outcome substantiates feasibility developing effective peptide combinations combat diseases related SARS-CoV-2. Moreover, three-peptide combinations, designed multiple aspects entry, exhibited heightened inhibition broad-spectrum properties.

Language: Английский

Citations

1
Characterization of the Pathogenic Features of Multiple SARS-CoV-2 Pandemic Strains in Different Mouse Models DOI

Huize Sun,

Kunpeng Liu,

Baocheng Yu

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
M protein ectodomain-specific immunity restrains SARS-CoV-2 variants replication DOI Creative Commons

Yibo Tang,

Kaiming Tang,

Yunqi Hu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 2, 2024

The frequent occurrence of mutations in the SARS-CoV-2 Spike (S) protein, with up to dozens mutations, poses a severe threat current efficacy authorized COVID-19 vaccines. Membrane (M) which is most abundant viral structural exhibits high level amino acid sequence conservation. M protein ectodomain could be recognized by specific antibodies; however, extent it immunogenic and provides protection remains unclear.

Language: Английский

Citations

0
Characterization of the Pathogenic Features of Multiple SARS‐CoV‐2 Pandemic Strains in Different Mouse Models DOI

Huize Sun,

Kunpeng Liu,

Baocheng Yu

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(11)

Published: Nov. 1, 2024

ABSTRACT Elucidating the detailed features of emerging SARS‐CoV‐2 strains both in vitro and vivo is indispensable for development effective vaccines or drugs against viral infection. We thoroughly characterized virological pathogenic eight different pandemic strains, from WT strain to current circulating sublineage EG.5.1, vivo. Besides observed Vero E6 cells, Omicron variants, BA.1 exhibited enhanced infectious effects upper respiratory tract K18 human angiotensin‐converting enzyme (ACE2) (K18 hACE2) transgenic mice. Both XBB.1.9.1 EG.5.1 presented stronger tropism brain, which could be main reason increased lethal on In addition, pathogenesis comparisons among all these viruses C57BL/6JGpt mice indicated that variant two new sublineages possessed dual tropisms mice, were further confirmed by subsequent bioinformatic analyses actual affinity comparison between RBDs mouse receptor ACE2. Furthermore, immunocompromised BKS‐db found more susceptible compared revealed infectivity was determined its host immunocompetence. Thus, this study not only contributes a systematic understanding but also provides insights combat potential future surges variants.

Language: Английский

Citations

0