HIV-associated penile anaerobes disrupt epithelial barrier integrity DOI Creative Commons
Lane B Buchanan,

Zhongtian Shao,

Ronald M. Galiwango

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(4), P. e1013094 - e1013094

Published: April 17, 2025

Specific anaerobic taxa within the penile microbiome—the Bacteria Associated with Seroconversion, Inflammation and Immune Cells (BASIC) species—enhance HIV-1 susceptibility, in part by recruiting susceptible cells to inner foreskin. However, their effect on epithelial barrier integrity has not been described. Using foreskin tissues swabs from 116 males undergoing voluntary medical male circumcision, we assessed relationship between BASIC species thickness, junction protein expression, cellular proliferation. The absolute abundance of was associated reduced tissue expression proteins claudin-1 E-cadherin, elevated soluble E-cadherin secretions, suggesting proteolytic cleavage. These effects were seen participants a high control without levels species. Prevotella bivia , but Peptostreptococcus anaerobius or Dialister micraerophilus shown directly degrade recombinant human increase release vitro . In vivo also linked thicker nucleated epithelium increased keratinocyte proliferation, no change stratum corneum thickness. Therefore, may enhance HIV susceptibility disrupting integrity, addition previously described target cell recruitment.

Language: Английский

HIV-associated penile anaerobes disrupt epithelial barrier integrity DOI Creative Commons
Lane B Buchanan,

Zhongtian Shao,

Ronald M. Galiwango

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(4), P. e1013094 - e1013094

Published: April 17, 2025

Specific anaerobic taxa within the penile microbiome—the Bacteria Associated with Seroconversion, Inflammation and Immune Cells (BASIC) species—enhance HIV-1 susceptibility, in part by recruiting susceptible cells to inner foreskin. However, their effect on epithelial barrier integrity has not been described. Using foreskin tissues swabs from 116 males undergoing voluntary medical male circumcision, we assessed relationship between BASIC species thickness, junction protein expression, cellular proliferation. The absolute abundance of was associated reduced tissue expression proteins claudin-1 E-cadherin, elevated soluble E-cadherin secretions, suggesting proteolytic cleavage. These effects were seen participants a high control without levels species. Prevotella bivia , but Peptostreptococcus anaerobius or Dialister micraerophilus shown directly degrade recombinant human increase release vitro . In vivo also linked thicker nucleated epithelium increased keratinocyte proliferation, no change stratum corneum thickness. Therefore, may enhance HIV susceptibility disrupting integrity, addition previously described target cell recruitment.

Language: Английский

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