Sex and Genotype Affect Mouse Hippocampal Gene Expression in Response to Blast-Induced Traumatic Brain Injury DOI Creative Commons
Kathleen E. Murray, Arun Reddy Ravula,

Victoria Stiritz

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Blast-induced traumatic brain injury (bTBI) has been identified as an increasingly prevalent cause of morbidity and mortality in both military civilian populations over the past few decades. Functional outcomes following bTBI vary widely among individuals, chronic neurodegenerative effects including cognitive impairments can develop without effective diagnosis treatment. Genetic predispositions sex differences may affect gene expression changes response to influence individual's probability sustaining long-term damage or exhibiting resilience tissue repair. Male female mice from eight genetically diverse distinct strains (129S1/SvImJ, A/J, C57BL/6J, CAST/EiJ, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, WSB/EiJ) which encompassed 90% genetic variability commercially available laboratory were exposed a single (180 kPa) using well-established shock tube system. Subacute hippocampal due blast exposure assessed RNA-seq at 1-month post-injury. We patterns dysregulation ontology terms canonical pathways related mitochondrial function, ribosomal structure, synaptic plasticity, protein degradation, intracellular signaling that varied by and/or strain, significant genes encoding respiratory complex I electron transport chain male WSB/EiJ glutamatergic synapse across more than half our groups. This study represents multi-level examination how provides foundation for identification potential therapeutic targets could be modulated improve health Veterans others with histories exposures.

Language: Английский

PFOS causes lysosomes-regulated mitochondrial fission through TRPML1-VDAC1 and oligomerization of MCU/ATP5J2 DOI

Wei Yang,

Yu Li,

Ruzhen Feng

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137685 - 137685

Published: Feb. 20, 2025

Language: Английский

Citations

0
ZLN005, a PGC-1α agonist, delays photoreceptor degeneration by enhancing mitochondrial biogenesis in a murine model of retinitis pigmentosa DOI Creative Commons

Chaojun Hu,

Chengda Ren, Yan Wu

et al.

Neuropharmacology, Journal Year: 2025, Volume and Issue: 269, P. 110361 - 110361

Published: Feb. 12, 2025

Retinitis pigmentosa (RP) is a hereditary neurodegenerative disease characterized by the degeneration of photoreceptors caused mutations in various genes. Increasing evidence suggests that mitochondrial biogenesis plays critical role many diseases. This study investigated rd1 mice, widely recognized model RP. Male C57BL/6 mice and age-matched were used for vivo experiments, while H2O2 was employed on 661w cells to establish an vitro model. Our findings revealed regulatory PGC-1α/NRF-1/TFAM pathway significantly downregulated mice. Treatment with ZLN005, PGC-1α agonist, markedly improved visual function alleviated thinning retinal outer nuclear layer. Additionally, ZLN005 enhanced restored photoreceptors. Further analysis confirmed rescued photoreceptor promoting through pathway. In summary, our results highlight progression offers potential strategy delay RP maintaining could be combined existing therapies improving treatment outcomes synergistic pathways.

Language: Английский

Citations

0
Mitochondrial fission – changing perspectives for future progress DOI Creative Commons
Sukrut C. Kamerkar, Ao Liu, Henry N. Higgs

et al.

Journal of Cell Science, Journal Year: 2025, Volume and Issue: 138(9)

Published: March 19, 2025

ABSTRACT Mitochondrial fission is important for many aspects of cellular homeostasis, including mitochondrial distribution, stress response, mitophagy, mitochondrially derived vesicle production and metabolic regulation. Several decades research has revealed much about fission, identification a key division protein – the dynamin Drp1 (also known as DNM1L) receptors on outer membrane (OMM), Mff, MiD49 MiD51 MIEF2 MIEF1, respectively) Fis1, regulators, post-translational modifications, actin filaments phospholipid cardiolipin. In addition, it now appreciated that other organelles, endoplasmic reticulum, lysosomes Golgi-derived vesicles, can participate in fission. However, more holistic understanding process lacking. this Review, we address three questions highlight knowledge gaps. First, how do quantify fission? Second, does inner (IMM) divide? Third, ‘types’ exist? We also introduce model integrates multiple regulatory factors mammalian model, possible pathways (cellular stimulation, switching or dysfunction) independently initiate recruitment at site, followed by shared second step which Mff mediates subsequent assembly contractile ring. conclude discussing some perplexing issues regulation, effects phosphorylation isoforms.

Language: Английский

Citations

0
Sex and Genotype Affect Mouse Hippocampal Gene Expression in Response to Blast-Induced Traumatic Brain Injury DOI Creative Commons
Kathleen E. Murray, Arun Reddy Ravula,

Victoria Stiritz

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Blast-induced traumatic brain injury (bTBI) has been identified as an increasingly prevalent cause of morbidity and mortality in both military civilian populations over the past few decades. Functional outcomes following bTBI vary widely among individuals, chronic neurodegenerative effects including cognitive impairments can develop without effective diagnosis treatment. Genetic predispositions sex differences may affect gene expression changes response to influence individual's probability sustaining long-term damage or exhibiting resilience tissue repair. Male female mice from eight genetically diverse distinct strains (129S1/SvImJ, A/J, C57BL/6J, CAST/EiJ, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, WSB/EiJ) which encompassed 90% genetic variability commercially available laboratory were exposed a single (180 kPa) using well-established shock tube system. Subacute hippocampal due blast exposure assessed RNA-seq at 1-month post-injury. We patterns dysregulation ontology terms canonical pathways related mitochondrial function, ribosomal structure, synaptic plasticity, protein degradation, intracellular signaling that varied by and/or strain, significant genes encoding respiratory complex I electron transport chain male WSB/EiJ glutamatergic synapse across more than half our groups. This study represents multi-level examination how provides foundation for identification potential therapeutic targets could be modulated improve health Veterans others with histories exposures.

Language: Английский

Citations

0