Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus DOI Creative Commons
Qianqian Xu,

Xiaomeng Ren,

Linzhen Hu

et al.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 17, 2025

Abstract To explore the chemical and biological diversities of diterpenoids from fungus Talaromyces adpressus , a previously unknown biosynthetic gene cluster (BGC, tdn ) for sordarin (a well-known fungal antibiotics) was discovered by leveraging genome mining method. Heterologous expressions key genes in Aspergillus oryzae led to determination one new diterpenoid, cycloaraneosene-9-ol-8-one ( 4 ), three known diterpenoids, cycloaraneosene 1 cycloaraneosene-9-ol 2 cycloaraneosene-8,9-diol 3 ). The structures – elucidated well via detailed analysis 1D 2D NMR, GCMS, HRESIMS, IR data, comparison with reported data. Structurally, compounds were belonging fusicoccane classical tricyclic 5/8/5 ring system, which are participated biosynthesis sordarin. Compound maybe precursor Baeyer–Villiger like reaction C8–C9 bond cleavage pathway Moreover, all isolates evaluated their bioactivities, exhibited inhibitory activities against human cancer cell lines IC 50 values ranging 7.8 32.4 µ M. promote apoptosis HCT-116 HepG2 cells, suppress migration cells. As well, also decrease expression proliferation related molecules BCL-2 cyclin D1, while increase BAX. Targets predication molecular docking indicate that compound exhibits stronger affinity DBL, suggesting its excellent binding potential. This finding will be enriched bioactivities most importantly, provide strategies synthetic research sordarins. Graphical

Language: Английский

Aculeanoids A–D, the second 17-nor fusicoccane diterpenoids with immunosuppressive activity from Aspergillus aculeatus DOI
Fei Liu, Qin Li, Yongqi Li

et al.

Phytochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 114414 - 114414

Published: Jan. 1, 2025

Language: Английский

Citations

0
Scaffold-Adaptive P450 Enzymes Balance Substrate Promiscuity with Catalytic Specificity in Brassicicene Biosynthesis DOI

Zhe Chen,

Wenling Yuan,

Fengli Li

et al.

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

We investigated the functional plasticity of two P450 enzymes, AbnK and AbnG, in brassicicene biosynthetic pathway. Through vivo heterologous expression, feeding assays, vitro reactions, we show that these enzymes regio- stereoselectively transform both 5–8–5 5–9–5 terpenoid scaffolds different ways, supported by computational simulations. also bridges a missing step A biosynthesis. Our findings demonstrate how controlled promiscuity underpins structural diversity, informing strategies to expand chemoenzymatic synthesis.

Language: Английский

Citations

0
Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus DOI Creative Commons
Qianqian Xu,

Xiaomeng Ren,

Linzhen Hu

et al.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 17, 2025

Abstract To explore the chemical and biological diversities of diterpenoids from fungus Talaromyces adpressus , a previously unknown biosynthetic gene cluster (BGC, tdn ) for sordarin (a well-known fungal antibiotics) was discovered by leveraging genome mining method. Heterologous expressions key genes in Aspergillus oryzae led to determination one new diterpenoid, cycloaraneosene-9-ol-8-one ( 4 ), three known diterpenoids, cycloaraneosene 1 cycloaraneosene-9-ol 2 cycloaraneosene-8,9-diol 3 ). The structures – elucidated well via detailed analysis 1D 2D NMR, GCMS, HRESIMS, IR data, comparison with reported data. Structurally, compounds were belonging fusicoccane classical tricyclic 5/8/5 ring system, which are participated biosynthesis sordarin. Compound maybe precursor Baeyer–Villiger like reaction C8–C9 bond cleavage pathway Moreover, all isolates evaluated their bioactivities, exhibited inhibitory activities against human cancer cell lines IC 50 values ranging 7.8 32.4 µ M. promote apoptosis HCT-116 HepG2 cells, suppress migration cells. As well, also decrease expression proliferation related molecules BCL-2 cyclin D1, while increase BAX. Targets predication molecular docking indicate that compound exhibits stronger affinity DBL, suggesting its excellent binding potential. This finding will be enriched bioactivities most importantly, provide strategies synthetic research sordarins. Graphical

Language: Английский

Citations

0