CRISPR/Cas Technology in Insect Insecticide Resistance DOI Creative Commons

Qiuchen Xu,

Mingyun Wang,

Jiahui Zeng

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 345 - 345

Published: March 26, 2025

Chemicals and biological insecticides play a crucial role as pest management strategies in modern agriculture forestry. However, their excessive unreasonable use inevitably leads to varying degrees of resistance among insect populations, which seriously affects the sustainability insecticide use. One primary reason for this is alterations or mutations gene expression. class genes encodes proteins that serve critical targets exert toxic effects insects, while another involved detoxification process within insects. Reverse genetics has become vital research tool studying molecular mechanisms underlying changes these target impact on resistance. The advent CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) CRISPR-associated Cas gene-editing technologies significantly advanced our understanding how insects adapt resist insecticides. This article aims provide comprehensive objective review progress made using CRISPR/Cas system various arthropods field control.

Language: Английский

Characterization of an Ecdysone Oxidase from Plutella xylostella (L.) and Its Role in Bt Cry1Ac Resistance DOI

Lina Dong,

Liuhong Zhu,

Zhouqiang Cheng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Understanding the molecular mechanisms underlying insect resistance to Bacillus thuringiensis (Bt) pesticidal proteins is crucial for sustainable pest management. Here, we found that downregulation of Plutella xylostella ecdysone oxidase gene (PxEO) in normal feeding stages contributes increased 20-hydroxyecdysone (20E) titer and mediates Bt Cry1Ac toxin. The PxEO was cloned its expression significantly downregulated midgut Bt-resistant Cry1Ac-selected P. xylostella. Silencing reduced susceptibility, closely linked protein metabolized (E) 20E vitro, reduction elevated titers activated MAPK-mediated trans-regulatory mechanism known directly cause phenotype. Together with our recently reported 20E-degrading glucose dehydrogenase, this finding highlights a robust, multipronged, approach developed by 20E-mediated defense against harmful agents.

Language: Английский

Citations

3
Molecular Mechanisms Underlying Resistance to Bacillus thuringiensis Cry Toxins in Lepidopteran Pests: An Updated Research Perspective DOI Creative Commons

De‐Qin Hu,

Dongmei Wang,

Hongsheng Pan

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 155 - 155

Published: Jan. 10, 2025

Genetically modified crops that produce insecticidal proteins from Bacillus thuringiensis (Bt) are currently the most efficient and safest method of pest control worldwide. However, prolonged planting period has led to a reduction in efficacy Bt due evolution resistance field. This review paper examines status lepidopteran pests under field conditions, elucidates molecular mechanism underlying their Cry toxins, discusses management strategies based on these mechanisms. Extensive research demonstrated mutations alterations expression patterns midgut receptor genes closely associated with resistance. As our understanding mechanisms progresses, several innovative approaches such as DNA detection techniques, engineering combining toxin RNAi technology have been developed for effective measures. Future will further unravel intricate this phenomenon develop scientifically sound integrated strategies.

Language: Английский

Citations

1
Microbial welan gum production, chemistry and applications: A review DOI
Huan Luo, Fan Wang,

Lei Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141640 - 141640

Published: March 1, 2025

Language: Английский

Citations

1
Nanomaterial-mediated RNAi reveals the effect of the oral secretory protein serine protease inhibitor on the growth of Conogethes punctiferalis larvae DOI
Yue Tong,

D Zhang,

Jie Ma

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 208, P. 106290 - 106290

Published: Jan. 7, 2025

Language: Английский

Citations

0
Reverse Engineering High-Level Resistance to Bt Cry1ac Toxin in Plutella Xylostella Reveals a Hormonal Regulatory Feedback Pathway DOI
Dan Sun, Mingyun Wang, Le Guo

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cuticle thickening mediates insecticide penetration resistance in Spodoptera litura DOI Creative Commons
Bingjie Wang,

Minghui Yi,

Mengyu Wang

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Functional analysis of the epsilon glutathione S-transferases in the adaptation of Spodoptera litura to xanthotoxin DOI

Tianxiang Xiao,

Mengqing Deng,

Xiaodan Huang

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: 180, P. 104299 - 104299

Published: March 10, 2025

Language: Английский

Citations

0
Insect metamorphosis and chitin metabolism under miRNA regulation: a review with current advances DOI Open Access

Ashraf Khan,

Guy Smagghe,

Shangwei Li

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Insect metamorphosis is a complex developmental process regulated by microRNAs (miRNAs) and hormonal signaling pathways. Key genes driving insect ontogenic changes are precisely modulated miRNAs, which interact with 20‐hydroxyecdysone (20E) juvenile hormone (JH) to coordinate transitions. Over the past decade, significant progress has been made in understanding miRNA biogenesis, their regulatory roles gene expression, involvement critical biological processes, including chitin metabolism. miRNAs now recognized as essential regulators of metabolism signaling, ensuring precise control development. Disrupting expression participating pathways through leads aberrant consequent lethal outcomes, highlighting potential targets for pest control. This review summarizes current advances miRNA‐mediated regulation metabolism, focus on interactions 20E JH By integrating recent findings, we provide insights into molecular mechanisms underlying function transitions applications management strategies. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Eukaryotic Translation Initiation Factor 2 Modulates the Expression of Midgut Receptors to Confer Resistance to Bacillus thuringiensis Cry1Ac Toxin in Helicoverpa armigera DOI
Caihong Zhang,

Jizhen Wei,

Wenxuan Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Studying the insect resistance mechanism to Bacillus thuringiensis (Bt) is beneficial address ever-growing problem of evolved resistance. Previous RNaseq data indicated that a eukaryotic translation initiation factor 2 (eIF2) expression showed significant differences in Cry1Ac-resistant Helicoverpa armigera strains. We investigated HaeIF2's role Cry1Ac Quantitative PCR (qPCR) confirmed HaeIF2 was significantly downregulated H. (BtR). Overexpression and RNAi midgut cells larvae affects susceptibility by modulating receptors CAD, ABCC2, ABCC3. Further studies demonstrated activates receptor binding eIF2 sites promoter regions. The three BtR consistent with reduced levels suggest involved These findings reveal due coordinated transcriptional regulation molecules strain, further expanding our understanding molecular basis Bt.

Language: Английский

Citations

0
Mother knows worst? Fungal infection enhances corn flavonoid of wogonin to inhibit Conogethes punctiferalis larval growth DOI Creative Commons
Qian Li, Jiayu Li,

Kaining Wu

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Summary Pathogen infection in host plants can alter the attraction and adaptability of herbivorous insects. Female adult insects often exhibit selective behaviours based on their environmental experiences, enabling offspring to avoid adverse conditions ensuring healthy growth development. However, comprehensive studies integrating both perspectives fitness plant validate significance such parental ‘Mother knows worst’ experiences remain limited. Building our previous findings that female Conogethes punctiferalis (Yellow peach moth, YPM) adults oviposition avoidance behaviour towards corn infected with Trichoderma asperellum , we further confirmed by T. significantly inhibits development YPM larvae. Feeding decreases larval gut microbiota diversity, core abundance led differential expression key genes juvenile hormone metabolic pathway. Moreover, content flavonoid wogonin, a secondary metabolite, was increased corn. In vitro feeding experiments revealed wogonin negatively impacts causing accumulation suppressing microbial strains. This study validates adaptive empiricism from perspective offspring, while elucidating mechanisms which microbial‐mediated resistance against insects, as well for exploring utilizing effective biocontrol resources YPMs.

Language: Английский

Citations

0