The Development of Mitochondrial Gene Editing Tools and Their Possible Roles in Crop Improvement for Future Agriculture DOI Creative Commons
Jinghua Yang, Xiaodong Yang, Tongbing Su

et al.

Advanced Genetics, Journal Year: 2021, Volume and Issue: 3(1)

Published: Dec. 13, 2021

We are living in the era of genome editing. Nowadays, targeted editing plant nuclear DNA is prevalent basic biological research and crop improvement since its first establishment a decade ago. However, achieving same accomplishment for mitochondrial has long been deemed impossible. Recently, pioneer studies on mitogenome have done using mitochondria-targeted transcription activator-like effector nucleases (mitoTALENs) rice, rapeseed, Arabidopsis. It well documented that mitochondria play essential roles development stress tolerance, particularly, cytoplasmic male sterility widely used production hybrids. The success enables studying fundamentals genome. Furthermore, RNA (mostly by nuclear-encoded pentatricopeptide repeat (PPR) proteins) sequence-specific manner can simultaneously change translatable mRNA. Moreover, direct nuclear-encoding factors required dynamics recombination may facilitate genetic manipulation mitochondria. Here, present state knowledge reviewed.

Language: Английский

The complete chloroplast genome sequence of Rhododendron farrerae Tate ex Sweet (Ericaceae) DOI Creative Commons

Jianshuang Shen,

Lu Huang

Mitochondrial DNA Part B, Journal Year: 2024, Volume and Issue: 9(1), P. 1 - 4

Published: Jan. 2, 2024

Tate ex Sweet 1831 is a species of ornamental plant found in southern China. In the present study, complete chloroplast genome

Language: Английский

Citations

2
Assessment on effects of transplastomic potato plants expressing Colorado potato beetle β-Actin double-stranded RNAs for three non-target pests DOI
Bailing Ren,

Jingnan Cao,

Yanqiu He

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2021, Volume and Issue: 178, P. 104909 - 104909

Published: July 1, 2021

Language: Английский

Citations

15
Multiple sgRNAs for one-step inactivation of the duplicatedacetyl-coenzyme A carboxylase 2(ACC2) genes inBrassica napus DOI Open Access
Lisa M. LaManna, Mugdha Parulekar, Pál Maliga

et al.

PLANT PHYSIOLOGY, Journal Year: 2022, Volume and Issue: 189(1), P. 178 - 187

Published: Feb. 17, 2022

Abstract Efficient plastid transformation in Arabidopsis (Arabidopsis thaliana) requires genetic lines that are hypersensitive to spectinomycin due the absence of a chloroplast acetyl-coenzyme A carboxylase (ACCase) encoded 2 (ACC2) nuclear gene. To obtain transformation-competent oilseed rape (Brassica napus), we inactivated all encoded, targeted ACCase copies using CRISPR–Cas9. Brassica napus (2n = 38, AACC) is recent interspecific hybrid rapa 20, AA) and B. oleracea 18, CC) expected have at least two ACC2 copies, one from each parent. The sequenced genome has rapa-like oleracea-like. We designed single-guide RNAs (sgRNAs) could simultaneously inactivate both copies. expressed Cas9 chimeric egg cell promoter 1.2 (EC1.2p) known yield homozygous or biallelic mutants T1 generation. maximize probability functionally inactivating orthologs single step, vectors carried four sgRNAs. Four T0 transgenic were obtained by Agrobacterium tumefaciens-mediated hypocotyl transformation. Amplicon sequencing confirmed mutations genes 10 progeny, seven which no wild-type (WT) copy remained. T2 seedlings lacking WT gene exhibited phenotype, suggesting they will be useful resource for

Language: Английский

Citations

7
Biotechnology Advances in Natural Food Colorant Acylated Anthocyanin Production DOI Creative Commons
Shaohua Zeng, Shuang Lin, Rong Jiang

et al.

Food Frontiers, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

ABSTRACT The food application of natural blue colorants such as acylated anthocyanin is becoming increasingly popular due to their safety, nutritional value, organoleptic attributes, and more pH/thermo‐stable properties compared nonacylated anthocyanins. However, it a great challenge obtain enough anthocyanins meet market demand, rather than readily available in nature from edible fruits vegetables. In this review, the crops, efficient eco‐friendly extraction food‐grade anthocyanins, potential intelligent packaging are extensively summarized. Subsequently, plant acyltransferases (AATs) also comprehensively strategy functional optimization AATs through computational or artificial intelligence–based protein‐directed evolution described suggested. Additionally, advantages disadvantages generated microbes suspension cells compared, chassis selection engineering discussed. Finally, approaches strategies metabolic enhance yield Taken together, review provides roadmap for production increasing demand future industry.

Language: Английский

Citations

1
The Development of Mitochondrial Gene Editing Tools and Their Possible Roles in Crop Improvement for Future Agriculture DOI Creative Commons
Jinghua Yang, Xiaodong Yang, Tongbing Su

et al.

Advanced Genetics, Journal Year: 2021, Volume and Issue: 3(1)

Published: Dec. 13, 2021

We are living in the era of genome editing. Nowadays, targeted editing plant nuclear DNA is prevalent basic biological research and crop improvement since its first establishment a decade ago. However, achieving same accomplishment for mitochondrial has long been deemed impossible. Recently, pioneer studies on mitogenome have done using mitochondria-targeted transcription activator-like effector nucleases (mitoTALENs) rice, rapeseed, Arabidopsis. It well documented that mitochondria play essential roles development stress tolerance, particularly, cytoplasmic male sterility widely used production hybrids. The success enables studying fundamentals genome. Furthermore, RNA (mostly by nuclear-encoded pentatricopeptide repeat (PPR) proteins) sequence-specific manner can simultaneously change translatable mRNA. Moreover, direct nuclear-encoding factors required dynamics recombination may facilitate genetic manipulation mitochondria. Here, present state knowledge reviewed.

Language: Английский

Citations

7