Genomic and cell-specific regulation of benzylisoquinoline alkaloid biosynthesis in opium poppy DOI Creative Commons
Uyen Vu Thuy Hong, Muluneh Tamiru‐Oli, Bhavna Hurgobin

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Opium poppy is a crop of great commercial value as source several opium alkaloids for the pharmaceutical industries including morphine, codeine, thebaine, noscapine, and papaverine. Most enzymes involved in benzylisoquinoline alkaloid (BIA) biosynthesis have been functionally characterized, currently serves model system to study BIA metabolism plants. involves two biosynthetic gene clusters associated respectively with morphine noscapine branches. Recent reports shown that genes same cluster are co-expressed, suggesting they might also be co-regulated. However, transcriptional regulation not well studied. three cell types phloem system: companion cells, sieve elements, laticifers. The transcripts distributed across types, requiring translocation key pathway intermediates between types. Together, these suggest multilayered complex, involving biochemical, genomic, physiological mechanisms. In this review, we highlight recent advances genome sequencing single spatial transcriptomics focus on how efforts can improve our understanding genomic cell-specific biosynthesis. Such knowledge vital genetic improvement metabolic engineering targeting modulation yield composition.

Language: Английский

How do plants defend themselves against pathogens-Biochemical mechanisms and genetic interventions DOI Creative Commons

Simardeep Kaur,

Mahesh Kumar Samota, Manoj Choudhary

et al.

Physiology and Molecular Biology of Plants, Journal Year: 2022, Volume and Issue: 28(2), P. 485 - 504

Published: Feb. 1, 2022

Abstract In agro-ecosystem, plant pathogens hamper food quality, crop yield, and global security. Manipulation of naturally occurring defense mechanisms in host plants is an effective sustainable approach for disease management. Various natural compounds, ranging from cell wall components to metabolic enzymes have been reported protect infection by hence provide specific resistance hosts against pathogens, termed as induced resistance. It involves various biochemical components, that play important role molecular cellular signaling events either before (elicitation) or after pathogen infection. The induction reactive oxygen species, activation defensive machinery comprising enzymatic non-enzymatic antioxidative secondary metabolites, pathogenesis-related protein expression (e.g. chitinases glucanases), phytoalexin production, modification composition, melatonin carotenoids accumulation, altered activity polyamines are major changes during Hence, the concentration restricts development. Such markers can be harnessed development “pathogen-proof” plants. Effective utilization key metabolites-based pave path candidate gene identification. This present review discusses valuable information understanding response mechanism cope with genomics-metabolomics-based proof cultivars along knowledge gaps future perspectives enhance agricultural production.

Language: Английский

Citations

263
Plant metabolic gene clusters in the multi-omics era DOI
Chuansong Zhan, Shuangqian Shen, Chenkun Yang

et al.

Trends in Plant Science, Journal Year: 2022, Volume and Issue: 27(10), P. 981 - 1001

Published: March 30, 2022

Language: Английский

Citations

102
Cepharanthine analogs mining and genomes of Stephania accelerate anti-coronavirus drug discovery DOI Creative Commons
Liang Leng, Zhichao Xu,

Bixia Hong

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 20, 2024

Abstract Cepharanthine is a secondary metabolite isolated from Stephania . It has been reported that it anti-conronaviruses activities including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we assemble three genomes ( S. japonica , yunnanensis and cepharantha ), propose the cepharanthine biosynthetic pathway, assess antiviral potential of compounds involved in pathway. Among genomes, near telomere-to-telomere assembly with one remaining gap, have chromosome-level assemblies. Following by gene mining metabolomics analysis, identify seven analogs broad-spectrum anti-coronavirus activities, SARS-CoV-2, Guangxi pangolin-CoV (GX_P2V), swine diarrhoea coronavirus (SADS-CoV), porcine epidemic diarrhea virus (PEDV). We also show two other genera, Nelumbo Thalictrum can produce analogs, thus for compound discovery. Results generated this study could accelerate drug

Language: Английский

Citations

35
Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops DOI Open Access
Miguel Ángel Villalobos-López, Analilia Arroyo-Becerra,

Anareli Quintero-Jiménez

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(19), P. 12053 - 12053

Published: Oct. 10, 2022

The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops not tolerant climatic environments. aim near future, a world with hunger an population, breed and/or engineer tolerate abiotic stress higher yield. Some crop varieties display certain degree of tolerance, which has been exploited by plant breeders develop thrive under conditions. Moreover, long list genes involved tolerance have identified characterized molecular techniques overexpressed individually transformation experiments. Nevertheless, phenotypes polygenetic traits, current genomic tools dissecting exploit their use accelerating genetic introgression using markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe mechanisms sense adverse conditions examine discuss classic new select improve crops.

Language: Английский

Citations

50
Plant secondary metabolites involved in the stress tolerance of long-lived trees DOI
Jiawen Cui, Xiang Li, Zhaogeng Lu

et al.

Tree Physiology, Journal Year: 2024, Volume and Issue: 44(2)

Published: Jan. 9, 2024

Abstract Ancient trees are natural wonders because of their longevity, having lived for hundreds or thousands years, and ability to withstand changing environments a variety stresses. These long-lived have sophisticated defense mechanisms, such as the production specialized plant metabolites (SPMs). In this review, we provide an overview major biotic abiotic stresses that often face, well analysis renowned ancient tree species unique protective SPMs against environmental stressors. We also discuss synthesis accumulation defensive induced by factors endophytes in these trees. Furthermore, conducted comparative genomic 17 discovered significant expansions SPM biosynthesis gene families species. Our comprehensive review reveals crucial role high resistance trees, providing novel resource defense, crop improvement even pharmaceutical industry.

Language: Английский

Citations

12
A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes DOI Creative Commons
Niraj R. Mehta, Yifan Meng, Richard N. Zare

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

12
The evolutionary trajectories of specialized metabolites towards antiviral defense system in plants DOI Creative Commons
Naveed Ahmad, Yi Xu, Faheng Zang

et al.

Molecular Horticulture, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 12, 2024

Abstract Viral infections in plants pose major challenges to agriculture and global food security the twenty-first century. Plants have evolved a diverse range of specialized metabolites (PSMs) for defenses against pathogens. Although, PSMs-mediated plant-microorganism interactions been widely discovered, these are mainly confined plant-bacteria or plant-fungal interactions. PSM-mediated plant-virus interaction, however, is more complicated often due additional involvement virus spreading vectors. Here, we review classes PSMs their emerging roles involved antiviral resistances. In addition, evolutionary scenarios between plant, virus-transmitting vectors presented. These advancements comprehending biochemical language during not only lay foundation understanding potential co-evolution across life kingdoms, but also open gateway fundamental principles biological control strategies beyond. Graphical The putative co-evolutionary triad plant (PSM) mediated viruses insect This dynamic trio depicted through interplay represented by pink green arrows, signifying PSM bidirectional occurring virus, host vector.

Language: Английский

Citations

11
Unraveling the specialized metabolic pathways in medicinal plant genomes: a review DOI Creative Commons
Mingcheng Wang, Shuqiao Zhang, Rui Li

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Medicinal plants are important sources of bioactive specialized metabolites with significant therapeutic potential. Advances in multi-omics have accelerated the understanding metabolite biosynthesis and regulation. Genomics, transcriptomics, proteomics, metabolomics each contributed new insights into biosynthetic gene clusters (BGCs), metabolic pathways, stress responses. However, single-omics approaches often fail to fully address these complex processes. Integrated provides a holistic perspective on key regulatory networks. High-throughput sequencing emerging technologies like single-cell spatial omics deepened our cell-specific spatially resolved dynamics. Despite advancements, challenges remain managing large datasets, standardizing protocols, accounting for dynamic nature metabolism, effectively applying synthetic biology sustainable production. This review highlights recent progress omics-based research medicinal plants, discusses available bioinformatics tools, explores future trends aimed at leveraging integrated improve quality utilization plant resources.

Language: Английский

Citations

9
Emerging mechanistic insights into the regulation of specialized metabolism in plants DOI
Louis‐Valentin Méteignier, Hans‐Wilhelm Nützmann, Nicolas Papon

et al.

Nature Plants, Journal Year: 2022, Volume and Issue: 9(1), P. 22 - 30

Published: Dec. 23, 2022

Language: Английский

Citations

32
Cultivated hawthorn (Crataegus pinnatifida var. major) genome sheds light on the evolution of Maleae (apple tribe) DOI Creative Commons
Ticao Zhang, Qin Qiao, Xiao Du

et al.

Journal of Integrative Plant Biology, Journal Year: 2022, Volume and Issue: 64(8), P. 1487 - 1501

Published: June 24, 2022

Cultivated hawthorn (Crataegus pinnatifida var. major) is an important medicinal and edible plant with a long history of use for health protection in China. Herein, we provide de novo chromosome-level genome sequence the cultivar "Qiu Jinxing." We assembled 823.41 Mb encoding 40 571 genes further anchored 779.24 into 17 pseudo-chromosomes, which account 94.64% genome. Phylogenomic analyses revealed that cultivated diverged from other species within Maleae (apple tribe) at approximately 35.4 Mya. Notably, involved flavonoid triterpenoid biosynthetic pathways have been significantly amplified In addition, our results indicated share unique ancient tetraploidization event; however, no recent independent whole-genome duplication event was specifically detected hawthorn. The amplification non-specific terminal repeat retrotransposons contributed most to expansion Furthermore, identified two paleo-sub-genomes extant found these sub-genomes showed different rearrangement mechanisms. also reconstructed ancestral chromosomes Rosaceae discussed possible paleo-polyploid origin patterns (autopolyploidization or allopolyploidization) Maleae. Overall, study provides improved context understanding evolution species, this new high-quality reference useful resource horticultural improvement

Language: Английский

Citations

31