Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis DOI
Olawale Samuel Adeyinka, Bushra Tabassum, Koloko Brice Landry

et al.

Planta, Journal Year: 2023, Volume and Issue: 257(4)

Published: March 13, 2023

Language: Английский

Cas12a Is Competitive for Gene Editing in the Malaria parasites DOI

Shijie Yang,

Yiming Wei,

Elvis Quansah

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107340 - 107340

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing promoter elements to enhance gene editing in plants: perspectives and advances DOI Creative Commons
Nikita Gondalia, Luis Felipe Quiroz, Linyi Lai

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Summary Genome‐edited plants, endowed with climate‐smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by promoters, which drives its transcription through interactions factors (TFs) and RNA polymerase. While constitutive promoters are extensively used in constructs, their limitations highlight need alternative approaches. This review emphasizes promise tissue/organ specific well inducible enable targeted spatiotemporal manner no effects on other tissues. Advances synthetic biology paved way creation offering refined control over expression augmenting potential GE. The integration these novel systems presents significant opportunities conditional genome editing. Moreover, advent bioinformatic artificial intelligence is revolutionizing characterization regulatory elements, enhancing our understanding roles plants. Thus, this provides insights into strategic use promoter to enhance precision, efficiency specificity GE, setting stage innovative crop improvement strategies.

Language: Английский

Citations

0
The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome DOI Creative Commons
Antonio Baldini, Francesco P. Battaglia, Giorgio Perrella

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Chromatin organization is a relevant layer of control gene expression during plant development. states strictly depend on associated features such as DNA methylation, histone modifications and variants. Thus, epigenome editing has become primary interest to alter without disrupting genomic sequences. Different tools have been developed address this challenge, starting with modular Zinc Finger Proteins (ZFPs) Transcription Activator Like Effectors (TALEs). However, the discovery CRISPR/Cas9 system adaptability technologies based enzymatically dead Cas9 (dCas9) paved way towards reliable adaptable in great variety organisms. In review, we will focus application targeted plants, summarizing most updated advances field. The promising results obtained by altering state targets involved flowering time abiotic stress resistance are crucial not only for elucidating molecular interactions that underly chromatin dynamics, but also future applications breeding programs an alternative route genetic manipulation achievement higher quality crops particularly terms nutritional properties, yield tolerance.

Language: Английский

Citations

0
Molecular Detection Methods for Forest Pathogens DOI
Jin Wu, Tingting Dai, Xizhuo Wang

et al.

Critical Reviews in Plant Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 23

Published: April 14, 2025

Language: Английский

Citations

0
Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis DOI
Olawale Samuel Adeyinka, Bushra Tabassum, Koloko Brice Landry

et al.

Planta, Journal Year: 2023, Volume and Issue: 257(4)

Published: March 13, 2023

Language: Английский

Citations

9