Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification DOI
Marlies Wouters, Héloïse Bastiaanse, Stéphane Rombauts

et al.

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(4), P. 3001 - 3016

Published: May 4, 2023

Abstract Arabidopsis (Arabidopsis thaliana) transfer DNA (T-DNA) insertion collections are popular resources for fundamental plant research. Cinnamoyl-CoA reductase 1 (CCR1) catalyzes an essential step in the biosynthesis of cell wall polymer lignin. Accordingly, intronic T-DNA mutant ccr1-6 has reduced lignin levels and shows a stunted growth phenotype. Here, we report restoration phenotype CCR1 expression after genetic cross with UDP-glucosyltransferase 72e1 (ugt72e1),-e2,-e3 mutant. We discovered that phenotypic recovery was not dependent on UGT72E family loss function but due to epigenetic phenomenon called trans suppression. Via suppression, gene restored introduction additional sharing identical sequences, leading heterochromatinization splicing out T-DNA–containing intron. Consequently, suppressed allele named epiccr1-6. Long-read sequencing revealed epiccr1-6, ccr1-6, carries dense cytosine methylation over full length T-DNA. showed SAIL UGT72E3 locus could trigger suppression GABI-Kat locus. Furthermore, scanned literature other potential cases found 22% publications matching our query double or higher-order mutants meet minimal requirements These combined observations indicate need be used caution since might derepress can thereby confound results.

Language: Английский

Systems genetic analysis of lignin biosynthesis in Populus tremula DOI Creative Commons

Mikko Luomaranta,

Carolin Grones,

Shruti Choudhary

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(6), P. 2157 - 2174

Published: July 28, 2024

Summary The genetic control underlying natural variation in lignin content and composition trees is not fully understood. We performed a systems analysis to uncover the regulation of biosynthesis ‘SwAsp’ population aspen ( Populus tremula ) trees. analyzed gene expression by RNA sequencing (RNA‐seq) differentiating xylem tissues, using Pyrolysis‐GC‐MS mature wood 268 from 99 genotypes. Abundant was observed for composition, genome‐wide association study identified proteins pentose phosphate pathway arabinogalactan protein glycosylation among top‐ranked genes that are associated with these traits. Variation polymorphism revealed through identification 312 705 local 292 003 distant quantitative trait loci (eQTL). A co‐expression network suggested modularization novel functions lignin‐biosynthetic CINNAMYL ALCOHOL DEHYDROGENASE 2 CAFFEOYL‐CoA O‐METHYLTRANSFERASE 3. PHENYLALANINE AMMONIA LYASE 3 co‐expressed HOMEOBOX PROTEIN 5 (HB5), role HB5 stimulating lignification demonstrated transgenic approach allowed linking trees´ responses external cues such as mechanical stimulus nutrient availability.

Language: Английский

Citations

1
Structure and Biomedical Properties of Lignins (A Review) DOI
А. П. Карманов,

A. V. Ermakova,

О. В. Раскоша

et al.

Russian Journal of Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 50(7), P. 2657 - 2674

Published: Dec. 1, 2024

Language: Английский

Citations

1
Glucuronic acid: not just another brick in the cell wall DOI Open Access
Dyoni Matias de Oliveira

New Phytologist, Journal Year: 2023, Volume and Issue: 238(1), P. 8 - 10

Published: March 2, 2023

This article is a Commentary on Debra‐Maceluch et al . (2023), 238 : 297–312.

Language: Английский

Citations

3
Structural features of stress lignin of aspen (Populus tremula L.) growing under increased background radiation DOI
А. П. Карманов, Л. М. Шапошникова,

Л. С. Кочева

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2023, Volume and Issue: 50, P. 102677 - 102677

Published: March 17, 2023

Language: Английский

Citations

3
Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification DOI
Marlies Wouters, Héloïse Bastiaanse, Stéphane Rombauts

et al.

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(4), P. 3001 - 3016

Published: May 4, 2023

Abstract Arabidopsis (Arabidopsis thaliana) transfer DNA (T-DNA) insertion collections are popular resources for fundamental plant research. Cinnamoyl-CoA reductase 1 (CCR1) catalyzes an essential step in the biosynthesis of cell wall polymer lignin. Accordingly, intronic T-DNA mutant ccr1-6 has reduced lignin levels and shows a stunted growth phenotype. Here, we report restoration phenotype CCR1 expression after genetic cross with UDP-glucosyltransferase 72e1 (ugt72e1),-e2,-e3 mutant. We discovered that phenotypic recovery was not dependent on UGT72E family loss function but due to epigenetic phenomenon called trans suppression. Via suppression, gene restored introduction additional sharing identical sequences, leading heterochromatinization splicing out T-DNA–containing intron. Consequently, suppressed allele named epiccr1-6. Long-read sequencing revealed epiccr1-6, ccr1-6, carries dense cytosine methylation over full length T-DNA. showed SAIL UGT72E3 locus could trigger suppression GABI-Kat locus. Furthermore, scanned literature other potential cases found 22% publications matching our query double or higher-order mutants meet minimal requirements These combined observations indicate need be used caution since might derepress can thereby confound results.

Language: Английский

Citations

3