Molecular Plant, Journal Year: 2023, Volume and Issue: 16(10), P. 1518 - 1546
Published: July 27, 2023
Language: Английский
Citations
24Genomics‐assisted breeding: The next‐generation wheat breeding era
Plant Breeding, Journal Year: 2023, Volume and Issue: 142(3), P. 259 - 268
Published: May 1, 2023
Abstract Common wheat provides approximately 20% of the total dietary calorie intake human beings. Recent technological advances in whole‐genome sequencing and their application its progenitor species provide new opportunities to uncover genetic variation traits accelerate traditional breeding (TB) strategies context genomics‐assisted (GAB). Integration TB, marker‐assisted selection (MAS) genomic (GS) with high‐density SNP markers is expected process further enhance gain. With assistance next‐ or third‐generation technologies high‐throughput phenotyping platforms, GAB can now realistically be considered following area: (i) genome high‐quality assembly variations, (ii) sequence‐based association studies, (iii) gene function (or functional gene) identification (iv) integration whole information, utilizing multi‐omics data different strategies. We argue that becoming preferred strategy pursuit cultivars superior on high yielding, nutritional quality, climate‐resilience so on.
Language: Английский
Citations
11Ancestral genome reconstruction and the evolution of chromosomal rearrangements in Triticeae
Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0Genomic structural variation in an alpha/beta hydrolase triggers hybrid necrosis in wheat
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 18, 2025
Hybrid necrosis, a century-old mystery in wheat, is caused by complementary genes Ne1 and Ne2. Ne2, encoding nucleotide-binding leucine-rich repeat (NLR) immune receptor, has been cloned, yet remains elusive. Here, we report that Ne1, which encodes an alpha/beta hydrolase (ABH) protein generated structural variation, triggers hybrid necrosis with Ne2 activating autoimmune responses. We further verify not only allelic variation but also copy number (CNV) of are pivotal for diversity wheat. likely originates from wild emmer potentially through duplication ectopic recombination events. Unlike frequently selected rust resistance wheat breeding, the lower prevalence modern cultivars attributed to its association necrosis. Altogether, these findings illuminate co-evolution NLR/ABH gene pair plant development innate immunity, offering potential benefits breeding. The underlying mechanism enigmatic. Si et al. demonstrated genetic interaction between α/β (Ne1) NLR receptor (Ne2) responses, inducing
Language: Английский
Citations
0A telomere-to-telomere genome assembly coupled with multi-omic data provides insights into the evolution of hexaploid bread wheat
Nature Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: April 7, 2025
The complete assembly of vast and complex plant genomes, like the hexaploid wheat genome, remains challenging. Here we present CS-IAAS, a comprehensive telomere-to-telomere (T2T) gap-free Triticum aestivum L. encompassing 14.51 billion base pairs featuring all 21 centromeres 42 telomeres. Annotation revealed 90.8 Mb additional centromeric satellite arrays 5,611 rDNA units. Genome-wide rearrangements, elements, transposable element expansion segmental duplications were deciphered during tetraploidization hexaploidization, providing understanding subgenome evolution. Among them, insertions hexaploidization greatly influenced gene expression balances, thus increasing genome plasticity transcriptional levels. Additionally, generated 163,329 full-length cDNA sequences proteomic data that helped annotate 141,035 high-confidence protein-coding genes. T2T reference (CS-IAAS), along with its transcriptome proteome, represents significant step in our complexity provides insights for future research breeding.
Language: Английский
Citations
0Functional characterization of TaWRKY254 in salt tolerance based on genome-wide analysis of the WRKY gene family in wheat core parent Zhou8425B
Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112540 - 112540
Published: May 1, 2025
Language: Английский
Citations
0TaRECQ4 contributes to maintain both homologous and homoeologous recombination during wheat meiosis
Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 29, 2024
Introduction Meiotic recombination (or crossover, CO) is essential for gamete fertility as well alleles and genes reshuffling that at the heart of plant breeding. However, CO remains a limited event, which strongly hampers rapid production original improved cultivars. RecQ4 gene encoding helicase protein that, when mutated, contributes to improve rate in all species where it has been evaluated so far. Methods In this study, we developed wheat ( Triticum aestivum L.) triple mutant (TM) three homoeologous copies TaRecQ4 mutants two heterozygous last one (Htz-A, Htz-B, Htz-D). Results Phenotypic observation revealed significant reduction pollen viability TM Htz-B plants compared wild type suggesting major defects during meiosis. Cytogenetic analyses these showed complete absence observed plants, leads chromosome fragmentation pachytene stage, resulting problems segregation chromosomes Htz-A Htz-D had an almost normal meiotic progression indicating both TaRecQ4-A TaRecQ4-D are functional there no dosage effect bread wheat. On contrary, TaRecQ4-B copy seems knocked-out, probably because SNP leading Threonine>Alanine change position 539 (T539A) protein, occurs crucial ATP bind/DEAD/ResIII domain unwinds nucleic acids. Occurrence numerous multivalents suggests could also play role control recombination. Discussion These findings provide foundation further molecular investigations into meiosis regulation fully understand underlying mechanisms how affects chiasma formation, identify ways mitigate enhance homologous efficiency
Language: Английский
Citations
2Bioinformatic Identification and Expression Analyses of the MAPK–MAP4K Gene Family Reveal a Putative Functional MAP4K10-MAP3K7/8-MAP2K1/11-MAPK3/6 Cascade in Wheat (Triticum aestivum L.)
Plants, Journal Year: 2024, Volume and Issue: 13(7), P. 941 - 941
Published: March 24, 2024
The mitogen-activated protein kinase (MAPK) cascades act as crucial signaling modules that regulate plant growth and development, response to biotic/abiotic stresses, immunity. MAP3Ks can be activated through MAP4K phosphorylation in non-plant systems, but this has not been reported plants date. Here, we identified a total of 234 putative TaMAPK family members wheat (Triticum aestivum L.). They included 48 MAPKs, 17 MAP2Ks, 144 MAP3Ks, 25 MAP4Ks. We conducted systematic analyses the evolution, domain conservation, interaction networks, expression profiles these TaMAPK–TaMAP4K (representing TaMAPK, TaMAP2K, TaMAP3K, TaMAP4K) members. TaMAPK–TaMAP4Ks are distributed on 21 chromosomes one unknown linkage group (Un). Notably, TaMAP4K possessed conserved motifs genes, including glycine-rich motif, invariant lysine (K) HRD DFG signature motif. TaMAPK3 6, TaMAP4K10/24 were shown strongly expressed only throughout development stages also drought or heat stress. bioinformatics qRT-PCR results suggested may activate MAP4K10–MEKK7–MAP2K11–MAPK6 pathway increase resistance wheat, MAP4K10–MAP3K8–MAP2K1/11-MAPK3 involved growth. In general, our work MAPK–MAP4K cascade profiled their potential roles during abiotic stresses based pattern. characterized might good candidates for future crop improvement molecular breeding.
Language: Английский
Citations
2Mapping of Aegilops speltoides derived leaf rust and stripe rust resistance genes using 35K SNP array
BMC Genomic Data, Journal Year: 2024, Volume and Issue: 25(1)
Published: July 15, 2024
Wheat is an essential food commodity cultivated throughout the world. However, this crop faces continuous threats from fungal pathogens, leaf rust (LR) and stripe (YR). To continue feeding growing population, these major destructors of wheat must be effectively countered by enhancing genetic diversity germplasm. In study, introgression line with hexaploid background (IL
Language: Английский
Citations
2Wheat genomics: genomes, pangenomes, and beyond
Trends in Genetics, Journal Year: 2024, Volume and Issue: 40(11), P. 982 - 992
Published: Aug. 26, 2024
Language: Английский
Citations
2