Single-cell transcriptome profiling reveals the spatiotemporal distribution of triterpenoid saponin biosynthesis and transposable element activity in Gynostemma pentaphyllum shoot apexes and leaves

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 8, 2024

Gynostemma pentaphyllum (Thunb.) Makino is an important producer of dammarene-type triterpenoid saponins. These saponins (gypenosides) exhibit diverse pharmacological benefits such as anticancer, antidiabetic, and immunomodulatory effects, have major potential in the pharmaceutical health care industries. Here, we employed single-cell RNA sequencing (scRNA-seq) to profile transcriptomes more than 50,000 cells derived from G. shoot apexes leaves. Following cell clustering annotation, identified five types four Each type displayed substantial transcriptomic heterogeneity both within between tissues. Examining gene expression patterns across various revealed that gypenoside biosynthesis predominantly occurred mesophyll cells, with heightened activity observed compared Furthermore, explored impact transposable elements (TEs) on landscapes. Our findings highlighted unbalanced certain TE families different leaves, marking first investigation at level plants. Additionally, dynamic genes involved specific during epidermal vascular development. The involvement regulating differentiation warrant further exploration. Overall, this study not only provides new insights into spatiotemporal organization leaves but also offers valuable cellular genetic resources for a deeper understanding developmental physiological processes resolution species.

Language: Английский

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Recent advances in paclitaxel biosynthesis and regulation

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Abstract Paclitaxel (PTX) is a high value plant natural product derived from Taxus (yew) species. This specialized metabolite (PSM) and its derivatives constitute cornerstone for the treatment of an increasing variety cancers. New applications PTX also continue to emerge, further promoting demand this WHO-designated essential medicine. Here we review recent advances in our understanding biosynthesis cognate regulation, which have been enabled by development transcriptomic approaches sequencing annotation three genomes. Collectively, has resulted elucidation two functional gene sets biosynthesis, unlocking new potential use heterologous hosts produce PTX. Knowledge pathway provides valuable resource regulation key PSM. Epigenetic PSM cell culture major concern production, given loss production long-term cultures. Recent developments aim design tools manipulating epigenetic potentially providing means reverse silencing caused DNA methylation. Exciting times clearly lie ahead improving potential.

Language: Английский

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Single-cell transcriptional profiling reveals cell type-specific responses to duck reovirus infection in the Bursa of Fabricius of Cairna moschata

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136391 - 136391

Published: Oct. 1, 2024

Language: Английский

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Current status of MSI research in Japan to measure the localization of natural products in plants

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102651 - 102651

Published: Oct. 19, 2024

Language: Английский

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Single-cell ATAC sequencing illuminates the cis-regulatory differentiation of taxol biosynthesis between leaf mesophyll and leaf epidermal cells in Taxus mairei

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 205, P. 117411 - 117411

Published: Sept. 14, 2023

Language: Английский

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