Transcriptome-wide m6A methylation profiling identifies GmAMT1;1 as a promoter of lead and cadmium tolerance in soybean nodules

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133263 - 133263

Published: Dec. 17, 2023

Language: Английский

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N6-methyladenosine RNA modification regulates photoperiod sensitivity in cotton

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 196(2), P. 1095 - 1109

Published: Aug. 7, 2024

Abstract The methylation of N6-methyladenosine (m6A) involves writers, erasers, and readers, acting synergistically in posttranscriptional regulation. These processes influence various biological processes, including plant floral transition. However, the specific role m6A modifications photoperiod sensitivity cotton (Gossypium hirsutum) remains obscure. To elucidate this, this study, we conducted transcriptome-wide sequencing during critical flowering transition stages photoperiod-sensitive wild G. hirsutum var. yucatanense (yucatanense) photoperiod-insensitive cultivated acc. TM-1 (TM-1). Our results revealed significant variations 2 varieties, with exhibiting elevated modification levels compared under long-day conditions. Notably, distinct peaks between correlated significantly sensitivity. Moreover, our study highlighted demethylase ALKB homolog 5 (GhALKBH5) modulating levels. Silencing GhALKBH5 led to a decreased mRNA level key photoperiodic genes (GhADO3, GhAGL24, GhFT1), resulting delayed bud emergence flowering. Reverse transcription quantitative PCR analyses confirmed that silencing GhADO3 GhAGL24 downregulated expression integrator GhFT1. Collectively, findings unveiled transcriptional regulatory mechanism which GhALKBH5-mediated demethylation crucial transcripts modulated cotton.

Language: Английский

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An mRNA methylase and demethylase regulate sorghum salt tolerance by mediating N6-methyladenosine modification

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract N 6-methyladenosine (m6A) modification is a crucial and widespread molecular mechanism governing plant development stress tolerance. The specific impact of m6A regulation on plants with inherently high salt tolerance remains unclear. Existing research primarily focuses the overexpression or knockout individual writer eraser components to alter levels. However, comprehensive study simultaneously altering overall levels within same experiment lacking. Such an investigation essential determine whether opposing changes in exert entirely different effects In this study, we identified major member mRNA adenosine methylase A (SbMTA) sorghum (Sorghum bicolor) as critical for survival. sbmta mutant exhibits phenotype characterized by reduced m6A, developmental arrest, and, ultimately, lethality. Overexpression SbMTA increased tolerance, while alkylated DNA repair protein AlkB homolog 10B (SbALKBH10B) showed opposite phenotype. Comparative analyses between reveal that SbMTA- SbALKBH10B-mediated alterations significantly stability expression genes related abscisic acid signaling pathway growth under stress. summary, unveils intricate relationship modifications sorghum, providing valuable insights into how transcripts influence responses

Language: Английский

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The Roles of RNA Modifications in Regulating Chloroplast Performance and Photosynthesis Efficiency

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11912 - 11912

Published: Nov. 6, 2024

The regulation of gene expression is crucial for maintaining cellular activities and responding to environmental stimuli. RNA molecules are central this regulatory network, influencing transcription, post-transcriptional processing, translation. Recent advancements have expanded our understanding modifications beyond the nucleus, highlighting their impact on chloroplast function photosynthesis efficiency. Chloroplasts, essential photosynthesis, rely precise genetic adapt changes. modifications, such as methylation pseudouridylation, critical in regulating stability, This review summarizes current knowledge how affect photosynthesis. It discusses roles specific occurring RNA, including N6-methyladenosine (m

Language: Английский

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