Current Issues in Molecular Biology,
Journal Year:
2024,
Volume and Issue:
46(10), P. 11086 - 11123
Published: Oct. 2, 2024
The
objective
of
this
systematic
review
(SR)
was
to
select
studies
on
the
use
gene
editing
by
CRISPR
technology
related
plant
resistance
biotic
stresses.
We
sought
evaluate
articles
deposited
in
six
electronic
databases,
using
pre-defined
inclusion
and
exclusion
criteria.
This
SR
demonstrates
that
countries
such
as
China
United
States
America
stand
out
with
CRISPR/Cas.
Among
most
studied
crops
are
rice,
tomatoes
model
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 6, 2025
Clubroot
disease
is
an
important
of
cruciferous
crops
worldwide
caused
by
Plasmodiophora
brassicae.
The
pathogen
P.
brassicae
can
infect
almost
all
crops,
resulting
in
a
reduction
yield
and
quality
the
host
plant.
first
part
this
review
outlines
process
infestation,
effectors,
physiological
pathotypes
identification
systems.
latter
highlights
summarizes
various
current
control
measures
research
progress
on
clubroot.
Finally,
we
propose
strategic
concept
for
sustainable
management
In
conclusion,
paper
will
help
to
deepen
knowledge
understanding
integrated
clubroot,
lay
solid
foundation
The Plant Journal,
Journal Year:
2025,
Volume and Issue:
122(1)
Published: April 1, 2025
SUMMARY
Clubroot
is
a
major
threat
to
cruciferous
crops
worldwide,
largely
due
the
complex
pathogenesis
of
its
causal
agent,
Plasmodiophora
brassicae
,
and
limited
availability
genetic
resistance
in
plants.
Previous
research
has
shown
that
P.
secretes
E3
ubiquitin
ligase
PbE3‐2,
which
targets
degrades
Arabidopsis
thaliana
cysteine
protease
RD21A
facilitate
infection.
In
this
study,
we
identified
plant
defense
mechanism
counteracts
pathogen
virulence
strategy.
We
found
A.
deubiquitinating
enzyme
OTU2,
whose
expression
upregulated
during
infection,
interacts
with
RD21A.
Notably,
OTU2
stabilized
by
deubiquitination
inhibited
interaction
between
PbE3‐2
Furthermore,
overexpression
enhanced
an
RD21A‐dependent
manner.
Collectively,
our
findings
demonstrate
deubiquitinates
RD21A,
protecting
it
from
PbE3‐2‐mediated
degradation
thereby
mitigating
virulence.
This
study
provides
new
insights
into
immune
mechanisms
offers
potential
strategies
for
developing
clubroot‐resistant
crops.
Physiologia Plantarum,
Journal Year:
2025,
Volume and Issue:
177(2)
Published: March 1, 2025
Abstract
As
one
of
the
most
important
crop
plants
worldwide,
understanding
mechanisms
underlying
grapevine
response
to
pathogen
attacks
is
key
achieving
a
productive
and
sustainable
viticulture.
Recently,
epigenetic
regulation
in
plant
immunity
has
gained
significant
traction
scientific
community,
not
only
for
its
role
gene
expression
but
also
heritability,
giving
it
enormous
biotechnological
potential.
Epigenetic
marks
have
been
shown
be
dynamically
modulated
genomic
regions
upon
infection,
with
some
being
maintained
after
such,
responsible
priming
defense
genes.
In
grapevine,
however,
knowledge
still
limited,
especially
regarding
biotic
stress
responses,
representing
glaring
gap
this
plant.
Here,
we
report
integrate
current
on
as
well
non‐epigenetic
non‐coding
RNAs
stress.
We
explore
how
may
useful
breeding
resistance,
considering
different
approaches,
from
uncovering
exploiting
natural
variation
inducing
through
means.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
ABSTRACT
Clubroot,
caused
by
Plasmodiophora
brassicae
,
poses
a
persistent
threat
to
Brassicaceae
crops,
particularly
in
regions
where
resistant
cultivars
are
under
strong
selection
pressure.
To
identify
new
sources
of
resistance
and
better
understand
the
underlying
genetic
mechanisms,
we
evaluated
60
Arabidopsis
thaliana
ecotypes
against
highly
virulent
Canadian
pathotype
3A.
Using
stringent
phenotyping
criteria,
pathogen
DNA
quantification,
survival
analysis,
identified
eight
ecotypes,
including
two
novel
sources,
Marce-1
DraII-6.
DraII-6
exhibited
exceptionally
low
disease
symptoms
high
rate.
While
gene
RPB1/WeiTsing
was
present
most
its
expression
significantly
elevated
at
early
infection
stages,
suggesting
potential
role
suppression.
However,
analysis
F1
F2
progeny
from
×
Col-0
cross
revealed
recessive
pattern,
supporting
hypothesis
that
RPB1
alone
may
not
be
sufficient
confer
clubroot
Our
findings
highlight
complexity
need
for
further
research
into
regulation
networks
beyond
RPB1,
context
translating
Arabidopsis-based
insights
Brassica
crops.
GRAPHICAL
Natural
variability
response
The
abstract
also
celebrates
first
author,
Melaine
Gonzalez-Garcia,
who
is
submitting
this
manuscript
just
one
week
before
welcoming
her
child.
Frontiers in Plant Science,
Journal Year:
2024,
Volume and Issue:
15
Published: May 29, 2024
The
resistance
of
Arabidopsis
thaliana
to
clubroot,
a
major
disease
Brassicaceae
caused
by
the
obligate
protist
Plasmodiophora
brassicae,
is
controlled
in
part
epigenetic
factors.
detection
some
these
quantitative
trait
loci
(QTLepi)
has
been
shown
depend
on
experimental
conditions.
aim
present
study
was
assess
whether
and
how
temperature
and/or
soil
water
availability
influenced
both
extent
effect
response
QTLepi.
recombinant
inbred
line
(epiRIL)
population,
derived
from
cross
between
ddm1-2
Col-0
(partially
resistant
susceptible
respectively),
phenotyped
for
P.
brassicae
under
four
abiotic
conditions
including
standard
conditions,
5°C
increase,
drought,
flooding.
constraints
tested
had
significant
impact
leaf
growth
epiRIL
population
outcome
epiRIL-pathogen
interaction.
Linkage
analysis
led
total
31
QTLepi,
18
which
were
specific
one
condition
13
common
at
least
two
environments.
EpiRIL
showed
plasticity
control,
appeared
be
traits
evaluated
These
results
highlight
that
environment
can
affect
architecture
plant
immune
responses
advance
our
understanding
factors
underlying
climate
change.
Phytopathology,
Journal Year:
2024,
Volume and Issue:
114(7), P. 1626 - 1636
Published: March 15, 2024
Development
of
durable
resistance
effective
against
a
broad
range
pathotypes
is
crucial
for
restoration
pathogen-damaged
ecosystems.
This
study
dissected
the
complex
genetic
architecture
limber
pine
quantitative
disease
(QDR)
to
Antioxidants,
Journal Year:
2024,
Volume and Issue:
13(12), P. 1553 - 1553
Published: Dec. 18, 2024
The
increase
in
extreme
climate
events
associated
with
global
warming
is
a
great
menace
to
crop
productivity
nowadays.
In
addition
abiotic
stresses,
warmer
conditions
favor
the
spread
of
infectious
diseases
affecting
plant
performance.
Within
this
context,
beneficial
microbes
constitute
sustainable
alternative
for
mitigation
effects
change
on
growth
and
productivity.
Used
as
biostimulants
improve
growth,
they
also
resistance
biotic
stresses
through
generation
primed
status
plant,
leading
better
faster
response
stress.
review,
we
have
focused
importance
balanced
redox
adequate
performance
revisited
different
antioxidant
mechanisms
supporting
biocontrol
effect
adjustment
levels
reactive
oxygen
species
(ROS).
addition,
tools
analysis
responses
regulation
been
evaluated.
activation
immune
mechanisms,
such
transcriptional
regulation,
retrograde
signaling,
post-translational
modification
proteins,
emerges
an
important
research
goal
understanding
activity
microbes.
