Agriculture,
Journal Year:
2024,
Volume and Issue:
14(12), P. 2301 - 2301
Published: Dec. 15, 2024
Adenophora
triphylla,
a
perennial
plant
widely
used
in
traditional
medicine,
produces
secondary
metabolites
like
lupenone
and
β-sitosterol.
Understanding
how
environmental
factors
influence
the
concentrations
of
these
is
crucial
for
optimizing
their
medicinal
use
cultivation.
This
study
examined
relationships
between
soil
physicochemical
properties,
climatic
variables,
growth
metabolite
content
A.
triphylla
roots
across
17
cultivation
sites
South
Korea.
Methods
included
UHPLC-MS/MS
analysis
quantifying
β-sitosterol,
alongside
correlation
traits
factors.
The
results
showed
that
higher
pH
calcium/magnesium
were
positively
correlated
with
fresh
weight
root,
whereas
lower
temperatures
increased
biomass
accumulation.
Additionally,
was
significantly
associated
elongation
various
root
growth,
β-sitosterol
only
diameter
taproot.
These
findings
suggest
factors,
particularly
pH,
Ca2+,
Mg2+,
temperature,
play
key
roles
influencing
both
production
providing
valuable
insights
into
improving
its
agronomic
potential.
Chemistry,
Journal Year:
2025,
Volume and Issue:
7(2), P. 36 - 36
Published: March 7, 2025
A
novel
series
of
oleanolic
acid
(OA,
1)
derivatives
incorporating
phenolic
and
coumarin
moieties
were
synthesized.
This
was
extracted
from
olive
pomace
(Olea
europaea
L.)
using
an
ultrasound-assisted
method.
The
structures
these
OA
characterized
through
the
utilization
1H-NMR,
13C-NMR
ESI-HRMS
analyses.
An
evaluation
some
biological
activities
prepared
conducted.
focused
principally
on
capacity
to
inhibit
15-lipoxygenase
α-glucosidase,
as
well
their
anticancer
properties
when
tested
against
tumour
cell
lines
(HCT-116
LS-174T)
a
non-tumour
line
(HEK-293).
In
terms
cytotoxic
activity,
majority
compounds
exhibited
notable
inhibitory
effects
compared
starting
molecule,
OA.
Derivatives
4d,
4k
4m
particularly
strong
HCT-116
line,
with
IC₅₀
values
38.5,
39.3,
40.0
µM,
respectively.
4l,
4e
5d
demonstrated
most
effective
inhibition
LS-174T
IC50
44.0,
44.3,
38.0
However,
compound
2a
effective,
exhibiting
potent
52.4
59.5
Furthermore,
molecular
docking
studies
supported
in
vitro
revealing
that
low
binding
energies
interacted
effectively
within
EGFR
enzyme’s
active
pocket
(PDB:
1M17).
These
findings
highlight
potential
agents
enzymatic
inhibitors,
warranting
further
investigation.
Organic Letters,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 12, 2025
Astracondensatol
D
(1),
a
pentacyclic
triterpenoid
featuring
an
uncommon
6/6/5/6-fused
ring
system,
along
with
its
precursor
astracondensatol
E
(2),
and
two
simplified
20(27)-octanorcycloastragenol
derivatives
(3
4)
were
isolated
from
Astragalus
condensatus
for
the
first
time.
Classical
NMR
spectroscopic
data,
integrated
DP4+
calculations,
unambiguously
determined
their
absolute
stereostructures.
X-ray
crystallography
provided
independent
confirmation
of
structure
compound
3.
The
plausible
biosynthetic
pathway,
encompassing
Wagner–Meerwein
rearrangement
retro-aldol
reaction,
was
proposed
formation
supported
by
computational
analysis.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(7), P. 3188 - 3188
Published: March 29, 2025
Plant
cell
suspension
cultures
offer
a
sustainable
method
for
producing
valuable
secondary
metabolites,
such
as
bioactive
pentacyclic
triterpenes.
This
study
established
high-triterpene-yielding
culture
from
the
apple
cultivar
“Cox
Orange
Pippin”.
Through
transcriptomic
analysis
and
triterpene
profiling
across
growth
phases,
we
uncovered
complex
regulatory
networks
that
govern
biomass
production
biosynthesis.
Key
biological
processes,
including
cycle
regulation,
wall
biosynthesis,
lipid
metabolism,
stress
response
mechanisms,
play
pivotal
roles
in
dynamics.
Differential
gene
expression
linked
to
these
processes
revealed
how
adapts
conditions
nutrient
availability
at
each
phase.
Methyl
jasmonate
elicitation
enhanced
phenylpropanoid
flavonoid
along
with
specific
pathways,
highlighting
its
potential
optimizing
metabolite
production.
enzymes,
oxidosqualene
cyclase
4
putative
C-2α
hydroxylase,
were
identified
promising
targets
future
metabolic
engineering
efforts.
represents
first
in-depth
report
on
molecular
mechanisms
underlying
plant
bioreactors,
specially
focusing
derived
semi-russeted
cultivar.
The
findings
reveal
key
pathways
accumulation
production,
offering
insights
bioreactor
industrial
applications.
