The chromosome-scale genome of Black Wolfberry (Lycium ruthenicum) provides useful genomic resources for identifying genes related to anthocyanin biosynthesis and disease resistance

Plant Diversity, Journal Year: 2025, Volume and Issue: 47(2), P. 201 - 213

Published: Jan. 6, 2025

The black wolfberry (L ycium ruthenicum; 2n = 2x 24) is an important medicinal plant with ecological and economic value. Its fruits have numerous beneficial pharmacological activities, especially those of anthocyanins, polysaccharides, alkaloids, high nutritional However, the lack available genomic resources for this species has hindered research on its evolutionary mechanisms. In study, we developed telomere-to-telomere (T2T) nearly gapless genome L. ruthenicum (2.26 Gb) by integrating PacBio HiFi, Nanopore Ultra-Long, Hi-C technologies. assembled comprised 12 chromosomes 37,149 protein-coding genes functionally annotated. Approximately 80% repetitive sequences were identified, which long terminal repeats (LTRs) most abundant, accounting 73.01%. abundance LTRs might be main reason larger compared to that other Lycium species. species-specific related defense mechanisms, salt tolerance, drought resistance, oxidative stress, further demonstrating their superior adaptability arid environments. Based fruit transcriptome data, constructed anthocyanin biosynthesis pathway identified 19 candidate structural seven transcription factors regulate in developmental stage ruthenicum, highly expressed at a later development. Furthermore, 154 potential disease resistance-related nucleotide-binding been genome. whole-genome proximal, dispersed, tandem duplication enriched number involved synthesis pathways. These results provide genetic basis understanding evolution pharmacologically active components genus.

Language: Английский

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Effects of biological soil crusts on plant growth and nutrient dynamics in the Minqin oasis-desert ecotone, Northwest China

Journal of Arid Land, Journal Year: 2025, Volume and Issue: 17(1), P. 130 - 143

Published: Jan. 1, 2025

Language: Английский

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Ammopiptanthus nanus Population Dynamics: Bridging the Gap Between Genetic Variation and Ecological Distribution Patterns

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 105 - 105

Published: Jan. 21, 2025

Ammopiptanthus nanus, a Tertiary-era endangered plant, is of great scientific value. In this research, we focus on A. nanus population dynamics in an effort to bridge the divide between micro genetic variation and macroscopic ecological pattern distribution. The structure 129 wild specimens from eight populations was analyzed using EST-SSR molecular markers research. Mantel test RDA analysis have been used research investigate factors that influence diversity nanus. Using 15 pairs SSR primers, total 227 alleles were detected samples 8 populations. mean number 17, average expected heterozygosity 0.405. It shown divided into six individual are significantly affected by wind speed terms genetics. suggested nature conservation area for be established as soon possible, based our results current natural distribution species. necessary issue pests diseases while simultaneously preventing continuation anthropogenic woodcutting disaster. Manual seedling collection should employed regions where environment permits. Through making use manual breeding techniques, will contribute growth

Language: Английский

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Response of different cotton genotypes to salt stress and re-watering

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 5, 2025

Abstract Background Cotton is a vital economic crop and reserve material pioneer planted on saline-alkaline soil. Improving the tolerance of cotton to saline alkaline environments particularly important. Results Salt-tolerant salt-sensitive plants at three-leaf stage were subjected 200 mM NaCl stress treatment, thereafter, microstructural observations beside physiological biochemical analyses performed leaves 0 h (CK), 48 (NaCl) re-watering (RW) for h. Salt altered in ST SS ( p < 0.05). After re-watering, recovered fully, while sustained permanent oxidative structural damage, indicating distinct salt tolerance. Transcriptome analysis was under conditions. KEGG revealed that response its adaptation may be related major protein families such as photosynthesis (ko 00195), photosynthesis-antenna 00196), plant hormone signal transduction 04075), starch sucrose metabolism 00500), porphyrin chlorophyll 00860). A gray coexpression module associated with restoration enriched according WGCNA. Conclusions did not only affect levels but also induced changes cells tissues. Re-watering relatively effective stabilizing parameters, well leaf microstructure, stress. WGCNA modules recovery stress, screening pivotal genes five critical hubs, namely, GH_A01G1528 , GH_A08G2688 GH_D08G2683 GH_D01G1620 GH_A10G0617 . Overall, our findings can provide new insights into enhancing exploring cotton,including genetic resources using those potential responsive genes. This study provides theoretical basis further exploration molecular mechanism breeding salt-tolerant cotton.

Language: Английский

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AmDEF2.7, a tandem duplicated defensin gene from Ammopiptanthus mongolicus, activated by AmWRKY14, enhances the tolerance of Arabidopsis to low temperature and osmotic stress

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 227, P. 105956 - 105956

Published: Aug. 27, 2024

Language: Английский

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Comparative transcriptome and coexpression network analysis revealed the regulatory mechanism of Astragalus cicer L. in response to salt stress

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 30, 2024

Astragalus cicer L. is a perennial rhizomatous legume forage known for its quality, high biomass yield, and strong tolerance to saline-alkaline soils. Soil salinization widespread environmental pressure. To use A. more scientifically environmentally in agriculture ecosystems, it highly important study the molecular response mechanism of salt stress.

Language: Английский

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The module consisting of transcription factor WRKY14 and thaumatin‐like protein TLP25 is involved in winter adaptation in Ammopiptanthus mongolicus

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(5)

Published: Sept. 1, 2024

Abstract Thaumatin‐like proteins (TLPs) are conserved involved in the defense and stress responses of plants. Previous studies showed that several TLPs were accumulated leaf apoplast Ammopiptanthus mongolicus winter, indicating might be related to adaptation winter climate A. . To investigate roles adaptation, we first analyzed expression pattern TLP genes then focused on biological function regulation pathway AmTLP25 gene. Several genes, including , upregulated during response both cold osmotic stress. Overexpression gene led an increased tolerance transgenic Arabidopsis freezing Furthermore, elevated AmWRKY14 transcription factor activated by specifically binding its promoter. It is speculated – module contributes temperate Our research advances current understanding regulatory provides valuable information for molecular mechanism evergreen broad‐leaved plants adapting climate.

Language: Английский

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Effects of dark septate endophyte on root growth, physiology and transcriptome of Ammopiptanthus mongolicus seedlings under drought stress

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 219, P. 109367 - 109367

Published: Nov. 29, 2024

Language: Английский

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Genome-wide identification and expression analysis of the WRKY gene family in Sophora flavescens during tissue development and salt stress

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 23, 2024

Sophora flavescens is a traditional Chinese medicinal herb rich in various bioactive secondary metabolites, such as alkaloids and flavonoids, exhibits remarkable resistance to abiotic stress. The WRKY transcription factor (TF) family one of the largest plant-specific TF families plays crucial role plant growth, development, responses However, comprehensive genome-wide analysis gene S. has not yet been conducted. In this study, we identified 69 SfWRKY genes from genome classified them into seven distinct subfamilies based on phylogenetic analysis. Transposed duplications dispersed were found be primary driving forces behind expansion family. Additionally, several cis -acting elements related stress response hormone signaling discovered within promoter regions SfWRKYs . Transcriptomic analyses across five tissues (leaves, flowers, pods, roots, stems) revealed that exhibiting high expression levels specific generally showed all examined tissues. Coexpression network constructed metabolomic transcriptomic root pod development indicated SfWRKY29 may play significant regulating biosynthesis metabolites during tissue development. RT-qPCR results significantly induced accumulation or salt Our study systematically analyzed TFs , which provides valuable reference data for further studies key roles growth well their under conditions.

Language: Английский

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AmChi7, an AmWRKY59 – activated chitinase, was involved in the adaption to winter climate in Ammopiptanthus mongolicus

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 219, P. 109436 - 109436

Published: Dec. 25, 2024

Language: Английский

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