Effect of a single rectal fecal microbiota transplantation on clinical severity and fecal microbial communities in dogs with chronic inflammatory enteropathy

Journal of Veterinary Internal Medicine, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 1, 2025

Abstract Background Fecal microbiota transplantation (FMT) has been advocated as a treatment for chronic enteropathy (CE) in dogs. However, so far only short‐term clinical effects have reported whereas the effect on remains unexplored. Hypothesis/Objectives Assess if single FMT enema can lead to improvement dogs with CE when accompanied by presumed favorable changes. The of glycerol cryopreservative storing preparations also was assessed. Animals Seven that received FMTs from 2 healthy donor Materials and Methods Six FMT, 1 dog 3 consecutive FMTs. Canine activity index (CCECAI) fecal samples were obtained before (Day 0), 7, 30 90 days after FMT. Samples stored without 10% glycerol. Sequencing (16S rRNA, Illumina) performed compared accepted analysis pipelines. Results Median CCECAI 8 (range, 5‐14), decreased median 1‐12) within week 0‐12) Day ( P < .01), an average duration response approximately 10 weeks. Significant variation donors' composition observed across different donations. Recipient or diversity did not change over time. Glycerol addition associated difference ≤ .001). Conclusions Clinical Importance A be considered appropriate CE, but consistent changes observed.

Language: Английский

---

Prolonged storage reduces viability of Peptacetobacter (Clostridium) hiranonis and core intestinal bacteria in fecal microbiota transplantation preparations for dogs

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Fecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial viability is unknown. We aimed (1) assess several core intestinal bacterial species by qPCR (2) Peptacetobacter (Clostridium) hiranonis through culture further characterize different preparations. Bacterial abundances were assessed feces from six healthy dogs after propidium monoazide (PMA-qPCR) selective quantitation viable bacteria. Conservation tested included lyophilization (stored at 4°C -20°C) freezing with glycerol-saline solution (12.5%) without any cryoprotectant -20°C). Additionally, abundance P. was quantified using culture. Using PMA-qPCR, Faecalibacterium, Escherichia coli, Streptococcus, Blautia, Fusobacterium, reduced lyophilized fecal samples kept -20°C up 6 months (p < 0.05). In frozen cryoprotectant, only Streptococcus E. coli not significantly 3 > Lastly, no differences observed those glycerol-preserved When evaluate hiranonis, we that lower than -20°C; higher preserved glycerol months. Moreover, highest feces. After months, undetectable 83% (5/6) glycerol. While procedure initially abundance, stable thereafter -20°C. The detected confirms use this a reliable method keep bacteria alive presence matrix purposes.

Language: Английский

---

Effect of faecal microbial transplantation on clinical outcome, faecal microbiota and metabolome in dogs with chronic enteropathy refractory to diet

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 8, 2025

Chronic enteropathy (CE) is a common complaint in canine gastroenterology. Recently, faecal microbiota transplantation (FMT) gained attention as treatment strategy. However, the efficacy and long-term impact of FMT still unclear. Clinical index (CIBDAI), metabolome were monitored 20 CE dogs refractory to diet before (T0) 3 months (T3) after FMT. Further data retrospectively collected up 1-year Significant improvements observed CIBDAI, Dysbiosis Index (DI), primary (PBAs) secondary (SBAs) bile acids propionate one month (T1) (CIBDAI (median range): T0 5 (1-9) vs. T1 1 (0-5), p < 0.0001; DI -0.1 (-5.6 3.8) -2.1 (-5.7 4.7), 0.05; PBAs decreased by 57%, SBAa increased 41%; 20%). According 17 clinically improved T3, 10 remained stable year Alpha- beta-diversity did not differ, neither nor FMT, from that healthy controls. The results highlight with mild clinical signs dysbiosis may benefit

Language: Английский

---

Chronic Enteropathy and Vitamins in Dogs

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 649 - 649

Published: Feb. 23, 2025

Chronic enteropathy (CE) or chronic inflammatory is a group of diseases with multiple and different etiologies characterized by gastrointestinal signs such as vomiting, diarrhea, anorexia, weight loss for more than 3 weeks, cell infiltration, lymphoplasmacytic cells in the intestinal mucosal lamina propria. The diagnosis was histologically confirmed after excluding other parasitic infections, tumors, pancreatitis, exocrine pancreatic insufficiency, metabolic diseases, endocrine hypoadrenocorticism. Nutritional management depends on several important functions, digestion absorption processes, digestive enzymes nutritional transporters, barrier functions. Intestinal dysbiosis may have been found to be involved various Recently, cobalamin (vitamin B12) vitamin D considered negative prognostic factors dogs CE. Cobalamin supplementation ameliorates clinical disease severity CE, hypocalcemia CE hypoalbuminemia. Therefore, aim this review provide an overview present treatment strategies vitamins.

Language: Английский

---

A Protocol for Fecal Microbiota Transplantation Using Freeze-Dried Capsules: Dosage and Outcomes in 171 Dogs with Chronic Enteropathy

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(2), P. 16 - 16

Published: April 15, 2025

Background: In veterinary medicine, fecal microbiota transplantation (FMT) shows promise for treating chronic enteropathy (CE) in dogs, but standardized protocols dosage, preparation, and administration are lacking. This study aimed to evaluate the efficacy of freeze-dried FMT capsules (cFMT) investigate existence a possible optimal dosage dogs with CE. Methods: A multicenter prospective was conducted on 171 CE, treated (100 mg ≤ 10 kg, 200 > kg). The material expressed different ways, effect putative active principles. Clinical outcomes were assessed by classifying as responders (R) or non-responders (NR) based evaluations from questionnaire, along changes CIBDAI score variations 15 clinical signs (CE). Data collected before days after treatment. Results: Of 111 included final analysis, 82% showed positive response, no significant differences response between capsule sizes irrespective how it expressed. Conclusion: Effective range cFMT affected CE defined. oral 100 daily month shown be sufficient achieve an 80% rate. Further studies needed explore additional factors that may influence overall effectiveness

Language: Английский

---

Changes in bacterial viability after preparation and storage of fecal microbiota transplantation solution using equine feces

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18860 - e18860

Published: Feb. 20, 2025

Fecal microbiota transplantation (FMT) has been used as a treatment option for horses (Equus caballus) with gastrointestinal diseases. Several preparation and conservation protocols to improve bacterial survival have studied in other species. This study aimed evaluate the impact of oxygen exposure different protectant solutions on viability before after freezing using horse feces. samples from 10 healthy were aliquoted diluted cryoprotectant containing antioxidants (n = 40) or 10% glycerol 40). Half aliquots each dilution condition prepared inside an anaerobic chamber, while half under ambient air conditions. Each sample was also analyzed fresh at -20 °C 90 days. Bacterial assessed flow cytometry. A mixed linear model Friedman Wilcoxon tests depending data distribution. Freeze-thawing decreased by 47% (mean ± SD: 51 27% before, 27 8% after; p < 0.001). Glycerol superior (32 glycerol, 24 cryoprotectant; Oxygen did not affect (p 0.13). There no statistical difference between 0.16). Fresh FMT may be better treating dysbiosis, but if cannot avoided, should dilute

Language: Английский

---

Preparation of Fecal Microbiota Transplantation Products for Companion Animals

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319161 - e0319161

Published: April 9, 2025

Fecal microbiota transplantation (FMT) is increasingly utilized in small animal medicine for the treatment of a variety gastrointestinal and non-gastrointestinal disorders. Despite proven clinical efficacy, there no detailed protocol available preparation storage FMT products veterinarians settings. Herein, effect processing technique on microbial community structure was assessed with amplicon sequence analysis. Microbial viability standard culture techniques using selective media. Given fastidious nature many intestinal microbes, colony forming units are considered surrogate viable representing portion potentially microbes. from four screened canine fecal donors six feline were processed aerobically according to double centrifugation adapted human medical literature. Fresh feces an additional three used evaluate cryopreservative, centrifugation, short-term vitro bacterial viability. Finally, fresh third group long-term frozen lyophilized products. Microbiota analysis revealed that each donor has unique profile. Processing does not significantly alter overall structure. The addition cryopreservatives lyopreservatives improved viability, up 6 months, compared unprocessed raw cryopreservative. These results prove practicality this approach veterinary provide researchers companion practitioners. Future vivo research needed how impacts recipient’s outcomes across multiple disease phenotypes.

Language: Английский

---

Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy

Journal of Veterinary Internal Medicine, Journal Year: 2025, Volume and Issue: 39(3)

Published: April 10, 2025

ABSTRACT Background Chronic enteropathies (CE) are common in cats, and alterations of the intestinal microbiota might be involved pathogenesis. Hypothesis/Objectives To evaluate efficacy a single enema fecal transplantation (FMT) improving dysbiosis clinical scores cats with CE. Animals Twenty‐eight either chronic inflammatory enteropathy (CIE; n = 19) or small cell gastrointestinal lymphoma (SCGL; 9) were prospectively enrolled. Methods Eleven randomly selected to receive FMT (FMT‐group), 17 used as controls. Clinical activity was determined using Feline Enteropathy Activity Index (FCEAI), feline index (DI) on day (T0) 30 days after (T1). Results At T0, 14/28 had an abnormal DI > 0. No significant difference found from T0 T1 group (mean[SD]: 0.01[2.5] vs. 0.7[2.1]; p 0.47). between control at −0.7[2.1] 0.8[1.8]; 0.92). FCEAI significantly decreased compared (median[IQR] 10.0[7.7–11.3] 4.5[4–5]; 0.002). 4.5[4–5] 4[3–5.75]; 0.64). Conclusions In this study, did not lead improvement CE

Language: Английский

---

Oral Fecal Microbiota Transplantation in Dogs with Tylosin-Responsive Enteropathy—A Proof-of-Concept Study

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(9), P. 439 - 439

Published: Sept. 18, 2024

A clinical trial was conducted to evaluate the effect of fecal microbiota transplantation (FMT) on canine chronic enteropathy activity index (CCECAI), consistency, and microbiome dogs with tylosin-responsive (TRE). The consisted four phases: (1) screening discontinuation tylosin for 4 weeks, (2) inclusion re-introduction 3-7 days, (3) treatment FMT/placebo (4) post-treatment follow-up weeks after cessation. study found that efficacy FMT (71.4%) slightly higher than placebo (50%), but this difference not statistically significant due underpowering. most abundant bacterial species detected in microbiomes TRE before or were

Language: Английский

---