Xenopus laevis as a Model Organism for the Study of Spinal Cord Formation, Development, Function and Regeneration DOI Creative Commons
Laura N. Borodinsky

Frontiers in Neural Circuits, Journal Year: 2017, Volume and Issue: 11

Published: Nov. 23, 2017

The spinal cord is the first central nervous system structure to develop during vertebrate embryogenesis, underscoring its importance organism. Because of early formation accessibility developing in amniotes challenging, often invasive and type experimental approaches amenable model systems like mammals are limited. In contrast, amphibians, general, African-clawed frog Xenopus laevis, particular, offer which cord, differentiation neurons glia establishment neuron neuromuscular synapses can be easily investigated with minimal perturbations whole significant advances on gene editing microscopy along recent completion laevis genome sequencing have reinvigorated use this classic species elucidate mechanisms formation, development, function regeneration.

Language: Английский

Xenbase: key features and resources of the Xenopus model organism knowledgebase DOI Creative Commons
Malcolm E Fisher, Christina James‐Zorn, Virgilio G. Ponferrada

et al.

Genetics, Journal Year: 2023, Volume and Issue: 224(1)

Published: Feb. 9, 2023

Abstract Xenbase (https://www.xenbase.org/), the Xenopus model organism knowledgebase, is a web-accessible resource that integrates diverse genomic and biological data from research on laboratory frogs laevis tropicalis. The goal of to accelerate discovery empower research, enhance impact data, facilitate dissemination these data. also enhances value through high-quality curation, integration, providing bioinformatics tools optimized for experiments, linking human other organisms. plays an indispensable role in making interoperable accessible broader biomedical community accordance with FAIR principles. provides annotated updates organizations such as NCBI, UniProtKB, Ensembl, Gene Ontology consortium, most recently, Alliance Genomic Resources, common clearing house humans This article brief overview key recently added features Xenbase. New include processing high-throughput sequencing NCBI Expression Omnibus; curation anatomical, physiological, expression phenotypes newly created Phenotype Ontology; annotations; new anatomical drawings Normal Table development; integration latest v10.1 genome annotations. Finally, we highlight areas future development at continue support community.

Language: Английский

Citations

61
Animal models of pain: Diversity and benefits DOI Creative Commons
Cynthia Abboud,

Alexia Duveau,

Rabia Bouali‐Benazzouz

et al.

Journal of Neuroscience Methods, Journal Year: 2020, Volume and Issue: 348, P. 108997 - 108997

Published: Nov. 11, 2020

Language: Английский

Citations

84
Xenbase: Facilitating the Use of Xenopus to Model Human Disease DOI Creative Commons
Mardi J. Nenni, Malcolm E Fisher, Christina James‐Zorn

et al.

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: Feb. 26, 2019

At a fundamental level most genes, signaling pathways, biological functions and organ systems are highly conserved between man all vertebrate species. Leveraging this conservation, researchers increasingly using the experimental advantages of amphibian Xenopus to model human disease. The online resource, Xenbase, enables disease modeling by curating literature published in PubMed integrating these data with orthologous anatomy, more recently links Online Mendelian Inheritance Man resource (OMIM) Human Disease Ontology (DO). Here we review how Xenbase supports report on meta-analysis research providing an overview different types diseases being modeled variety approaches used. Text mining over 50,000 articles imported into from identified approximately 1,000 putative disease- articles. These were manually assessed annotated ontologies, which then used classify papers based type. We found that is study diverse array three main approaches: cell-free egg extracts aspects cellular molecular biology, oocytes ion transport channel physiology embryo experiments focused congenital diseases. integrated Pages allow easy navigation information external databases. Results analysis will equip suite available or dissect pathological process. Ideally clinicians basic use foster collaborations necessary interrogate development treatment

Language: Английский

Citations

76
Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adult Xenopus laevis DOI Creative Commons
Nirosha J. Murugan, Hannah J. Vigran, Kelsie A. Miller

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(4)

Published: Jan. 26, 2022

Limb regeneration is a frontier in biomedical science. Identifying triggers of innate morphogenetic responses vivo to induce the growth healthy patterned tissue would address needs millions patients, from diabetics victims trauma. Organisms such as Xenopus laevis —whose limited regenerative capacities adulthood mirror those humans—are important models with which test interventions that can restore form and function. Here, we demonstrate long-term (18 months) regrowth, marked repatterning, functional restoration an amputated X. hindlimb following 24-hour exposure multidrug, pro-regenerative treatment delivered by wearable bioreactor. Regenerated tissues composed skin, bone, vasculature, nerves significantly exceeded complexity sensorimotor untreated control animals’ hypomorphic spikes. RNA sequencing early buds revealed activation developmental pathways Wnt/β-catenin, TGF-β, hedgehog, Notch. These data successful “kickstarting” endogenous vertebrate model.

Language: Английский

Citations

43
Genetic approaches for increasing fitness in endangered species DOI
Tiffany A. Kosch, Anthony W. Waddle, Caitlin Cooper

et al.

Trends in Ecology & Evolution, Journal Year: 2022, Volume and Issue: 37(4), P. 332 - 345

Published: Jan. 10, 2022

Language: Английский

Citations

40
Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists DOI Creative Commons

Elisa C. H. van Doorn,

Jorik H. Amesz, Amir H. Sadeghi

et al.

Cardiovascular Engineering and Technology, Journal Year: 2024, Volume and Issue: 15(2), P. 232 - 249

Published: Jan. 16, 2024

Abstract For recent decades, cardiac diseases have been the leading cause of death and morbidity worldwide. Despite significant achievements in their management, profound understanding disease progression is limited. The lack biologically relevant robust preclinical models that truly grasp molecular underpinnings its pathophysiology attributes to this stagnation, as well insufficiency platforms effectively explore novel therapeutic avenues. area fundamental translational research has therefore gained wide interest scientists clinical field, while landscape rapidly evolved towards an elaborate array modalities, characterized by diverse distinctive traits. As a consequence, current literature lacks intelligible complete overview aimed at focuses on selecting optimal platform for questions. In review, we present vitro, ex vivo, vivo silico model health disease, delineating main benefits drawbacks, innovative prospects, foremost fields application scope incentives.

Language: Английский

Citations

11
<b><i>Xenopus</i></b>: An Undervalued Model Organism to Study and Model Human Genetic Disease DOI Open Access
Martin Blum, Tim Ott

Cells Tissues Organs, Journal Year: 2018, Volume and Issue: 205(5-6), P. 303 - 313

Published: Jan. 1, 2018

The function of normal and defective candidate genes for human genetic diseases, which are rapidly being identified in large numbers by geneticists the biomedical community at large, will be best studied relevant predictive model organisms that allow high-speed verification, analysis underlying developmental, cellular molecular mechanisms, establishment disease models to test therapeutic options. We describe discuss pros cons frog Xenopus, has been extensively used uncover developmental mechanisms past, but is underutilized as a model. argue Xenopus complements more commonly mouse zebrafish time- cost-efficient animal study alleles mechanisms.

Language: Английский

Citations

83
Precision Medicine in the Management of Dilated Cardiomyopathy DOI Creative Commons
Diane Fatkin,

Inken G. Huttner,

Jason C. Kovacic

et al.

Journal of the American College of Cardiology, Journal Year: 2019, Volume and Issue: 74(23), P. 2921 - 2938

Published: Dec. 1, 2019

Language: Английский

Citations

75
Tissue-Specific Gene Inactivation inXenopus laevis: Knockout oflhx1in the Kidney with CRISPR/Cas9 DOI Open Access
Bridget D. DeLay, Mark E. Corkins, Hannah L. Hanania

et al.

Genetics, Journal Year: 2017, Volume and Issue: 208(2), P. 673 - 686

Published: Nov. 30, 2017

Abstract Xenopus laevis is a classic developmental model, but its allotetraploid genome has limited our ability to perform genetic manipulations. The advance of... Studying genes involved in organogenesis often difficult because many of these are also essential for early development. frog, laevis, commonly used study processes, the presence two homeologs genes, it been use as model. Few studies have successfully CRISPR amphibians, and currently there no tissue-targeted knockout strategy described Xenopus. goal this determine whether CRISPR/Cas9-mediated gene can be targeted kidney without perturbing function. We demonstrate that targeting editing eye F0 embryos feasible. Our shows both lhx1 results disruption development function does not lead defects. Therefore, may necessary bypass defects reported upon Lhx1 protein expression or by morpholinos, antisense RNA, dominant negative constructs. establish control (slc45a2) when knocked out albinism altering This establishes feasibility tissue-specific Xenopus, providing cost-effective efficient method assessing roles implicated abnormalities amenable high-throughput drug screening techniques.

Language: Английский

Citations

69
The African clawed frog Xenopus laevis : A model organism to study regeneration of the central nervous system DOI
Dasfne Lee‐Liu, Emilio E. Méndez‐Olivos, Rosana Muñoz

et al.

Neuroscience Letters, Journal Year: 2016, Volume and Issue: 652, P. 82 - 93

Published: Oct. 1, 2016

Language: Английский

Citations

67