Nanoparticle‐mediated double‐stranded RNA delivery system: A promising approach for sustainable pest management

Insect Science, Journal Year: 2020, Volume and Issue: 28(1), P. 21 - 34

Published: June 1, 2020

Abstract RNA interference (RNAi) targeting lethal genes in insects has great potential for sustainable crop protection. Compared with traditional double‐stranded (ds)RNA delivery systems, nanoparticles such as chitosan, liposomes, and cationic dendrimers offer advantages delivering dsRNA/small interfering (si)RNA to improve RNAi efficiency, thus promoting the development practice of RNAi‐based pest management strategies. Here, we illustrate limitations dsRNA reveal mechanism nanoparticle‐mediated RNAi, summarize recent progress successful applications management, finally address prospects nanoparticle‐based pesticides.

Language: Английский

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Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens

Insect Biochemistry and Molecular Biology, Journal Year: 2020, Volume and Issue: 123, P. 103428 - 103428

Published: June 14, 2020

Language: Английский

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CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model Insect Pyrrhocoris apterus

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: July 15, 2019

The CRISPR/Cas9 technique is widely used in experimentation with human cell lines as well other model systems, such mice Mus musculus, zebrafish Danio reiro, and the fruit fly Drosophila melanogaster. However, publications describing use of for genome editing non-model organisms, including insects, are scarce. introduction this relatively new method presents many problems even experienced researchers, especially lack procedures to tackle issues concerning efficiency mutant generation. Here we present a protocol efficient insect species Pyrrhocoris apterus. We collected data from several independent trials that targeted genes using system determined crucial optimization steps led remarkably increased production. main follows: timing embryo injection, heteroduplex mobility assay screening method, vivo testing sgRNA efficiency, G0 germline mosaicism screening. egg injections here need be optimized species, but here-described solutions can applied immediately species.

Language: Английский

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Changes in juvenile hormone titres and differential expression of related genes at different stages of Coccinella septempunctata L. female adults supplied with an artificial diet and aphid diet

Bulletin of Entomological Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 7

Published: Jan. 8, 2025

Abstract Juvenile hormone (JH) regulates multiple physiological functions in insects including growth, metamorphosis, and reproduction. epoxide hydrolase (JHEH) juvenile esterase (JHE) are degradative enzymes that metabolise JH, JH receptor (methoprene-tolerant, Met ) the regulation of female reproduction vitellogenesis. In this study, titres Coccinella septempunctata adult females were determined using ultra high-performance liquid chromatography tandem mass spectrometry; ranged from 0.03 to 0.16 ng g −1 5- 30-day-old adults. JHEH, JHE , expression studied different reproductive stages C. by quantitative real-time PCR. JHEH transcription levels highest 25-day-old adults 1.93-fold higher than 5-day-old inhibited addition artificial diet. supplied with 3 μl diet was similar an aphid diet, results showed supplementation 582.2 most suitable for . The study provide important insights improvement diets.

Language: Английский

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Silencing of LIM homeodomain transcription factor 1 alpha (Lmx1a) gene caused larval molting failure and adult reproductive deficiency in Henosepilachna vigintioctopunctata

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

LIM-HD (homeodomain) protein is essential for the specific differentiation of various cells and tissues due to its conserved HD. Within LIM subclass, transcription factor 1 alpha (Lmx1a) remains poorly characterized in insects. This study investigates expression patterns HvLmx1a 28-spotted lady beetle, Henosepilachna vigintioctopunctata, at both developmental stage tissue levels. Furthermore, RNA interference (RNAi)-mediated knockdown resulted increased mortality during early larval stage. Injection dsHvLmx1a beginning 4th instar caused a reduction 20E titer, disrupted normal molting pupation processes, led emergence larval-pupal abnormalities. Notably, was significantly down-regulated on d 2 4 post-injection, which coincided with marked inhibition HvCHS1 genes associated bursicon signaling pathways. Additionally, silencing induced qualitative changes male testes female ovaries, resulting infertility mortality. Ultimately, these findings suggest that influences ovarian morphology development through lipid metabolism. In conclusion, this provides first evidence diverse physiological roles Lmx1a H. vigintioctopunctata highlight potential as target RNAi-based biological control strategies.

Language: Английский

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Halloween genes are expressed with a circadian rhythm during development in prothoracic glands of the insect RHODNIUS PROLIXUS

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2024, Volume and Issue: 290, P. 111588 - 111588

Published: Jan. 18, 2024

We analyse the developmental and circadian profiles of expression genes responsible for ecdysteroidogenesis (Halloween genes) in PGs Rhodnius prolixus throughout larval-adult development. Extensive use vitro techniques enabled multiple different parameters to be measured individual PGs. Expression disembodied spook closely paralleled ecdysteroid synthesis same PGs, titre vivo, but with functionally significant exceptions. Various tissues other than expressed one, both or neither genes. Both gonads express pharate adults (larvae close ecdysis). were at low, significant, levels UF Rhodnius, raising questions concerning how arrest is maintained animals. IHC confirmed subcellular localisation coded proteins. Gene knockdown suppressed transcription synthesis, apparently regulating downstream gene disembodied. Transcription occurred a daily rhythm (with peaks night) that was under control using aperiodic conditions. The complex behaviour LL implied two anatomically distinct oscillators regulate this rhythm. First, clock second, PTTH which released rhythmically from brain therein, described previously. conclude employs similar pathway as insects, its complex, involving mechanisms within outside

Language: Английский

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Nanoparticle facilitated stacked-dsRNA improves suppression of the Lepidoperan pest Chilo suppresallis

Pesticide Biochemistry and Physiology, Journal Year: 2022, Volume and Issue: 187, P. 105183 - 105183

Published: July 27, 2022

Language: Английский

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The loss of Halloween gene function seriously affects the development and reproduction of Diaphorina citri (Hemiptera: Liviidae) and increases its susceptibility to pesticides

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 191, P. 105361 - 105361

Published: Feb. 3, 2023

Language: Английский

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RNA-seq analysis of gene expression changes during pupariation in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

BMC Genomics, Journal Year: 2018, Volume and Issue: 19(1)

Published: Sept. 21, 2018

The oriental fruit fly, Bactrocera dorsalis (Hendel) has been considered to be one of the most important agricultural pest around world. As a holometabolous insect, larvae must go through metamorphosis process with dramatic morphological and structural changes complete their development. To better understand molecular mechanisms these changes, RNA-seq B. from wandering stage (WS), late (LWS) white puparium (WPS) were performed. In total, 11,721 transcripts obtained, out which 1914 genes (578 up-regulated 1336 down-regulated) 2047 (655 1392 found differentially expressed between WS LWS, as well WPS, respectively. Of DEGs, 1862 1996 successfully annotated in various databases. analysis data together qRT-PCR validation indicated that during this transition, oxidative phosphorylation pathway, encoding P450s, serine protease inhibitor, cuticular proteins down-regulated, while up-regulated. Moreover, we some 20-hydroxyecdysone (20E) biosynthesis signaling pathway had higher expression WS, responsible for juvenile hormone (JH) synthesis, degradation, transporter pathways suggesting might involved larval pupariation dorsalis. For chitinolytic enzymes, chitinases (chitinase 2, chitinase 5, 8, 10) chitin deacetylase play crucial role degradation insect expressions significantly increased transition. Here, also synthase 1A synthesis cuticles Significant at transcriptional level identified Importantly, obtained vast quantity associated genes, would all help us mechanism

Language: Английский

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Characteristics of Halloween genes and RNA interference‐mediated functional analysis of LmCYP307a2 in Locusta migratoria

Insect Science, Journal Year: 2021, Volume and Issue: 29(1), P. 51 - 64

Published: Feb. 25, 2021

Halloween genes are involved in the biosynthesis of molting hormone, which plays a key role insect ecdysis, development, metamorphosis, and reproduction. Our previous work identified five from Locusta migratoria, but their functions currently unknown. In this study, sequences these were analyzed characterized. LmCYP307a2, LmCYP306a1, LmCYP302a1, LmCYP315a1 primarily expressed prothoracic glands, while LmCYP314a1 was universally peripheral tissues, especially ovaries Malpighian tubules. All mainly 5th to 7th d 5th-instar nymphs. RNA interference (RNAi) silencing LmCYP307a2 resulted severe delays failure, could be rescued by supplementary 20-hydroxyecdysone. A hematoxylin eosin staining analysis suggested that RNAi inhibited ecdysis process inhibiting apolysis degradation old cuticle, promoting synthesis new cuticle. Quantitative reverse transcription polymerase chain reaction results showed expressions LmE74, LmCht5, LmCht10 dramatically down-regulated, LmChsI substantially up-regulated, after knockdown LmCYP307a2. The suggest is related molt via regulation chitin degradation.

Language: Английский

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